Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion

Autor: Sébastien Houy, Joana S Martins, Noa Lipstein, Jakob Balslev Sørensen

Publikováno v: eLife, Vol 11 (2022)

Munc13 proteins are priming factors for SNARE-dependent exocytosis, which are activated by diacylglycerol (DAG)-binding to their C1-domain. Several Munc13 paralogs exist, but their differential roles are not well understood. We studied the interdepen

Externí odkaz: https://doaj.org/article/d0398067d21b464997bebef8c94a870b

Author response: Phorbolester-activated Munc13-1 and ubMunc13-2 exert opposing effects on dense-core vesicle secretion

Autor: Joana S Martins, Sébastien Houy, Noa Lipstein, Jakob Balslev Sørensen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c3b69d7474180e96f05a426f112b038b
https://doi.org/10.7554/elife.79433.sa2

Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2- and Ca2+-dependent priming

Autor: Cordelia Imig, Nils Brose, Paulo S. Pinheiro, Joana S Martins, Jakob B. Sørensen, Benjamin H. Cooper, Sonja M. Wojcik, Bassam Tawfik, Sébastien Houy

Publikováno v: Tawfik, B, Martins, J S, Houy, S, Imig, C, Pinheiro, P S, Wojcik, S M, Brose, N, Cooper, B H & Sørensen, J B 2021, ' Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2-and Ca2+-dependent priming ', eLife, vol. 10, e64527 . https://doi.org/10.7554/eLife.64527
eLife
eLife, Vol 10 (2021)

Synaptotagmins confer calcium-dependence to the exocytosis of secretory vesicles, but how coexpressed synaptotagmins interact remains unclear. We find that synaptotagmin-1 and synaptotagmin-7 when present alone act as standalone fast and slow Ca2+-se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3572c076493c0ae85e6c83a513bc7dce
https://curis.ku.dk/ws/files/286521602/Synaptotagmin_7_places_dense_core_vesicles_at_the_cell_membrane_to_promote_Munc13_2_and_Ca2_dependent_priming_publisher_version_.pdf

Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells

Autor: Sébastien, Houy, Joana S, Martins, Ralf, Mohrmann, Jakob Balslev, Sørensen

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2233

Fusion of vesicles with the plasma membrane and liberation of their contents is a multistep process involving several proteins. Correctly assigning the role of specific proteins and reactions in this cascade requires a measurement method with high te

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::caa00aa98e49db82f88e9fd43b306cbb
https://pubmed.ncbi.nlm.nih.gov/33222139

SNAPPy: A snakemake pipeline for scalable HIV-1 subtyping by phylogenetic pairing

Autor: Pedro Araújo, Joana S. Martins, Nuno S. Osório

Publikováno v: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Virus Evolution

Human immunodeficiency virus 1 (HIV-1) genome sequencing is routinely done for drug resistance monitoring in hospitals worldwide. Subtyping these extensive datasets of HIV-1 sequences is a critical first step in molecular epidemiology and evolution s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5dd9e86e168056292309bb9ab44b1a55
https://doi.org/10.1093/ve/vez050