Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Joan I. Speirs"'
Publikováno v:
Journal of food protection. 54(4)
In rice, milk, and brain-heart-infusion cultures, 17 of 67 Bacillus cereus strains produced a heat-stable toxin causing morphological changes in cultures cells. All of the positive strains were associated with illness, eight with the emetic syndrome.
Publikováno v:
Canadian Journal of Microbiology. 37:877-880
Two commercial agglutination kits, a reserved passive agglutination test (VET-RPLA) and a staphylococcal coagglutination test (Phadebact ETEC-LT Test), were compared with two cell culture assays (Y-1 and Vero) and GM1 ganglioside enzyme-linked immuno
Autor:
M. Akhtar, Joan I. Speirs
Publikováno v:
Canadian Journal of Microbiology. 37:650-653
Sandwich enzyme-linked immunosorbent assays (ELISAs) were developed to detect Escherichia coli cytotoxins. Wells were coated with monoclonal antibodies from hybridomas 13C4 and (or) 11E10, and biotin conjugates of these antibodies were used for detec
Publikováno v:
Canadian Journal of Microbiology. 37:647-650
Five strains of nonpathogenic Listeria monocytogenes were characterized for (i) hemolysin production, (ii) cytolysis of Chinese hamster ovary (CHO) cells, and (iii) ability to attach and enter intestine 407 cells. Four of the five strains produced va
Publikováno v:
Letters in applied microbiology. 14(2)
A commercial competitive enzyme immunoassay kit, Escherichia coli ST EIA, was compared with the conventional infant mouse assay for sensitivity and specificity in detecting E. coli heat-stable enterotoxin. Thirty-one of 46 strains of E. coli tested w
Autor:
Joan I. Speirs, Jack Konowalchuk
Publikováno v:
Journal of Milk and Food Technology. 38:469-472
Survival of enteric viruses under several environmental conditions was studied to determine whether contaminated vegetables pose a health hazard. Five test viruses stored in water or phosphate-buffered saline survived without significant loss for 36
Autor:
Joan I. Speirs, Jack Konowalchuk
Publikováno v:
Journal of Food Science. 41:1013-1017
Inactivation of poliovirus with strawberry extract was time and concentration dependent and could be prevented or reduced but not reversed with serum or gelatin. Strawberry extract inactivated several enteric viruses and herpes simplex virus, and ext
Publikováno v:
Applied and Environmental Microbiology. 53:2666-2670
Polioviruses and rotaviruses are potential indicators of sewage pollution of water and shellfish. Several methods for detecting these viruses in oysters were assessed. Elution-precipitation involving Catfloc for clarification and skim milk for subseq
Autor:
S. Stavric, Joan I. Speirs
Publikováno v:
Canadian Institute of Food Science and Technology Journal. 22:205-208
The first outbreak of hemorrhagic colitis due to Escherichia coli 0157:H7 in Canada occurred in 1982. There were 31 cases with one death in a home for the aged; undercooked hamburger was the suspected vehicle. The serotype 0157 :H7 had rarely been en
Publikováno v:
Journal of AOAC INTERNATIONAL. 71:675-678
A previously described monoclonal antibody-enzyme immunoassay system for dairy products was examined to determine its potential for detecting Listeria in naturally contaminated ground meats. In addition, a microtiter plate system was developed which