Výsledky vyhledávání - "João L. Lagarto"

Akademický článek

Multispectral Depth-Resolved Fluorescence Lifetime Spectroscopy Using SPAD Array Detectors and Fiber Probes

Autor: João L. Lagarto, Caterina Credi, Federica Villa, Simone Tisa, Franco Zappa, Vladislav Shcheslavskiy, Francesco Saverio Pavone, Riccardo Cicchi

Publikováno v: Sensors, Vol 19, Iss 12, p 2678 (2019)

Single Photon Avalanche Diode (SPAD) arrays are increasingly exploited and have demonstrated potential in biochemical and biomedical research, both for imaging and single-point spectroscopy applications. In this study, we explore the application of S

Externí odkaz: https://doaj.org/article/d4f7c5bd41f14626ae2e69cffe172854

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Akademický článek

An automated multiwell plate reading flim microscope for live cell autofluorescence lifetime assays

Autor: Douglas J. Kelly, Sean C. Warren, Sunil Kumar, João L. Lagarto, Benjamin T. Dyer, Anca Margineanu, Eric W.-F. Lam, Chris Dunsby, Paul M. W. French

Publikováno v: Journal of Innovative Optical Health Sciences, Vol 7, Iss 5, Pp 1450025-1-1450025-15 (2014)

Fluorescence lifetime imaging (FLIM) is increasingly used to read out cellular autofluorescence originating from the coenzyme NADH in the context of investigating cell metabolic state. We present here an automated multiwell plate reading FLIM microsc

Externí odkaz: https://doaj.org/article/5ca8fba2d865405390f39b3fd9b0e54b

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Structural and Biochemical Changes in Pericardium upon Genipin Cross-Linking Investigated Using Nondestructive and Label-Free Imaging Techniques

Autor: Tanveer Ahmed Shaik, Enrico Baria, Xinyue Wang, Florian Korinth, João L. Lagarto, Christiane Höppener, Francesco S. Pavone, Volker Deckert, Jürgen Popp, Riccardo Cicchi, Christoph Krafft

Publikováno v: Analytical Chemistry. 94:1575-1584

Tissue cross-linking represents an important and often used technique to enhance the mechanical properties of biomaterials. For the first time, we investigated biochemical and structural properties of genipin (GE) cross-linked equine pericardium (EP)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07024bafe31c9fb635c138a21d30d11b
https://doi.org/10.1021/acs.analchem.1c03348

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Characterization of NADH fluorescence properties under one-photon excitation with respect to temperature, pH, and binding to lactate dehydrogenase

Autor: Taylor M. Cannon, Edwin Garcia, Alexander R. Lyon, Nicholas S. Peters, Christopher Dunsby, João L. Lagarto, Paul M. W. French, Douglas J. Kelly, Benjamin T. Dyer

Publikováno v: Osa Continuum

Reduced nicotinamide adenine dinucleotide (NADH) is the principal electron donor in glycolysis and oxidative metabolism and is thus recognized as a key biomarker for probing metabolic state. While the fluorescence characteristics of NADH have been in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::319c341caea3b097cb73212e4e3779dc
https://pubmed.ncbi.nlm.nih.gov/34458690

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Monitoring changes in biochemical and biomechanical properties of collagenous tissues using label-free and nondestructive optical imaging techniques

Autor: Tanveer Ahmed Shaik, Enrico Baria, Melis Goktas, Patrick Igoche Onoja, Kerstin Blank, João L. Lagarto, Francesco S. Pavone, Jürgen Popp, Christoph Krafft, Riccardo Cicchi

Publikováno v: Analytical chemistry (Wash.) 93 (2021): 3813–3821. doi:10.1021/acs.analchem.0c04306
info:cnr-pdr/source/autori:Shaik, Tanveer Ahmed; Lagarto, Joao L.; Baria, Enrico; Goktas, Melis; Onoja, Patrick Igoche; Blank, Kerstin G.; Pavone, Francesco S.; Popp, Juergen; Krafft, Christoph; Cicchi, Riccardo/titolo:Monitoring changes in biochemical and biomechanical properties of collagenous tissues using label-free and nondestructive optical imaging techniques/doi:10.1021%2Facs.analchem.0c04306/rivista:Analytical chemistry (Wash.)/anno:2021/pagina_da:3813/pagina_a:3821/intervallo_pagine:3813–3821/volume:93
Analytical Chemistry

We demonstrate the ability of nondestructive optical imaging techniques such as second-harmonic generation (SHG), two-photon fluorescence (TPF), fluorescence lifetime imaging (FLIM), and Raman spectroscopy (RS) to monitor biochemical and mechanical a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3caefb75a5fc08269197a8ae7df487af
https://pubs.acs.org/doi/10.1021/acs.analchem.0c04306

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Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues

Autor: João L. Lagarto, Riccardo Cicchi, Francesco S. Pavone, Vladislav I. Shcheslavskiy

Publikováno v: Optics letters. 45(8)

We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f0c4cbccae82f5ab95022c09e28f0e2
https://pubmed.ncbi.nlm.nih.gov/32287205

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Fiber-based macroscale fluorescence lifetime imaging for real-time in situ tissue diagnostics (Conference Presentation)

Autor: Francesco S. Pavone, Vladislav I. Shcheslavskiy, João L. Lagarto, Riccardo Cicchi

Publikováno v: Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis.

The inability to efficiently separate autofluorescence signals from bright background illumination is a key issue that hinders widespread deployment of optical techniques such as time-correlated single photon counting (TCSPC) in clinical settings. We

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::286d857606baba4b20ba8068ad46e17e
https://doi.org/10.1117/12.2543010

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Real-time diagnosis and visualization of tumor margins in excised breast specimens using fluorescence lifetime imaging and machine learning

Autor: Jennifer E. Phipps, Christoph Hebisch, João L. Lagarto, Hanna Kim, Laura Marcu, Richard J. Bold, Morgan A. Darrow, Jakob Unger

Publikováno v: Biomedical optics express, vol 11, iss 3

Tumor-free surgical margins are critical in breast-conserving surgery. In up to 38% of the cases, however, patients undergo a second surgery since malignant cells are found at the margins of the excised resection specimen. Thus, advanced imaging tool

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c07df736bca9fd681b59c5d303e61d4
https://escholarship.org/uc/item/77m19692

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Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays

Autor: Francesco S. Pavone, Riccardo Cicchi, Franco Zappa, Federica Villa, Vladislav I. Shcheslavskiy, João L. Lagarto, Simone Tisa

Publikováno v: Scientific Reports
Scientific reports (Nature Publishing Group) 10 (2020): 8116-1–8116-10. doi:10.1038/s41598-020-65218-3
info:cnr-pdr/source/autori:Lagarto, João L.; Villa, Federica; Tisa, Simone; Zappa, Franco; Shcheslavskiy, Vladislav; Pavone, Francesco S.; Cicchi, Riccardo/titolo:Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays/doi:10.1038%2Fs41598-020-65218-3/rivista:Scientific reports (Nature Publishing Group)/anno:2020/pagina_da:8116-1/pagina_a:8116-10/intervallo_pagine:8116-1–8116-10/volume:10
Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)

Autofluorescence spectroscopy has emerged in recent years as a powerful tool to report label-free contrast between normal and diseased tissues, both in vivo and ex vivo. We report the development of an instrument employing Single Photon Avalanche Dio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9dad1f1a1b0e16ec6f78edbff191bbb4
https://pubmed.ncbi.nlm.nih.gov/32415224

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