Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Jiu Tsair Tsay"'
Autor:
Robert G. Brankamp, Phil L. Smith, Keith E. Kropp, Jiu-Tsair Tsay, Lori A. Birkenberger, Jonathan D. Wierschke, A Subramaniam, Dale T. Blankenship, Koti Sreekrishna
Publikováno v:
Gene. 190:55-62
Numerous heterologous proteins have been produced at greater than gram per liter levels in the methylotrophic yeast, Pichia pastoris, using the methanol oxidase promoter. The factors that drastically influence protein production in this system includ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::610bda9872c2bd2e09a561528abe5f9a
https://doi.org/10.1016/s0378-1119(96)00672-5
https://doi.org/10.1016/s0378-1119(96)00672-5
Publikováno v:
Europe PubMed Central
Thiolactomycin (TLM) and cerulenin are antibiotics that block Escherichia coli growth by inhibiting fatty acid biosynthesis at the beta-ketoacyl-acyl carrier protein synthase I step. Both TLM and cerulenin trigger the accumulation of intracellular ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6da2537853b9473e4387afc33e3a7cd8
https://doi.org/10.1128/jb.175.12.3723-3729.1993
https://doi.org/10.1128/jb.175.12.3723-3729.1993
Plasmids that corrected the temperature-sensitive unsaturated fatty acid auxotrophy of strain M6 [fabA6 (Ts)] were isolated from an Escherichia coli genomic library. Subcloning and physical mapping localized the new gene (called sfa for suppressor of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78cae7516be7b28df4627af26a79c983
https://europepmc.org/articles/PMC178354/
https://europepmc.org/articles/PMC178354/
Publikováno v:
Biochemistry. 31(14)
There is marked pH dependence of the rate constant (koff) for tetrahydrofolate (H4folate) dissociation from its ternary complex with human dihydrofolate reductase (hDHFR) and NADPH. Similar pH dependence of H4folate dissociation from the ternary comp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::358269e22f993ed24aede6da332ee008
https://pubmed.ncbi.nlm.nih.gov/1314649
https://pubmed.ncbi.nlm.nih.gov/1314649
Autor:
Jiu Tsair Tsay, Tavner J. Delcamp, Raymond L. Blakley, James R. Appleman, William A. Beard, Neal J. Prendergast, James H. Freisheim
Publikováno v:
Biochemistry. 29(27)
The role of the active site residue phenylalanine-31 (Phe31) for recombinant human dihydrofolate reductase (rHDHFR) has been probed by comparing the kinetic behavior of wild-type enzyme (wt) with mutant in which Phe31 is replaced by leucine (F31L rHD
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::342a5992aa2af65b10e6c479117d6d38
https://pubmed.ncbi.nlm.nih.gov/2207084
https://pubmed.ncbi.nlm.nih.gov/2207084
Autor:
William L. Mock, Jiu Tsair Tsay
Publikováno v:
Journal of the American Chemical Society. 111:4467-4472
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b4cc9455963e31ad3dff6c2703d8f6b8
https://doi.org/10.1021/ja00194a049
https://doi.org/10.1021/ja00194a049
Autor:
Jiu Tsair Tsay, William L. Mock
Publikováno v:
Biochemistry. 25:2920-2927
The substrate analogue 2-(1-carboxy-2-phenylethyl)-4-phenylazophenol is a potent competitive inhibitor of carboxypeptidase A. Upon ligation to the active site, the azophenol moiety undergoes a shift of pKa from a value of 8.76 to a value of 4.9; this
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad26dc2859729042f774a1f5f8875137
https://doi.org/10.1021/bi00358a028
https://doi.org/10.1021/bi00358a028
Autor:
Jiu-Tsair Tsay, William L. Mock
Publikováno v:
Synthetic Communications. 18:769-776
2-Benzyl-3-(methylsulfoximino)propionic acid has been prepared from 2-benzyl-3-(methylthio)propionamide by cyclization to a isothiazolinone hydrochloride with N-chlorosuccinimide, followed by peracid oxidation and acidic hydrolysis.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9cea49f2bb24e40ca600a8e29071ac32
https://doi.org/10.1080/00397918808057844
https://doi.org/10.1080/00397918808057844
Autor:
William L. Mock, Jiu-Tsair Tsay
Publikováno v:
Journal of Biological Chemistry. 263:8635-8641
The phenolic group of active site residue Tyr-248 in carboxypeptidase A has a pKa value of 10.06, as determined from the pH dependence of its rate of nitration by tetranitromethane. The decrease in enzyme activity (kcat/Km) in alkaline solution, char
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fa1502a2660691a79f602935e9bbe755
https://doi.org/10.1016/s0021-9258(18)68352-8
https://doi.org/10.1016/s0021-9258(18)68352-8
Autor:
Jiu-Tsair Tsay, William L. Mock
Publikováno v:
ChemInform. 19
2-Benzyl-3-(methylsulfoximino)propionic acid has been prepared from 2-benzyl-3-(methylthio)propionamide by cyclization to a isothiazolinone hydrochloride with N-chlorosuccinimide, followed by peracid oxidation and acidic hydrolysis.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::95a641780c614ea72d06204218a678a5
https://doi.org/10.1002/chin.198848145
https://doi.org/10.1002/chin.198848145