Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Jin H. Bae"'
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
The chemical stability of DNA makes complete erasure of DNA-encoded data difficult. Here the authors mix true and false messages, differentiated by whether a truth marker oligo is bound to it, and show that brief exposure to elevated temperatures ran
Externí odkaz:
https://doaj.org/article/521caa4ca79b45d38fd209aba923c447
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-11 (2016)
DNA hybridisation thermodynamics parameters underlie rational design of oligonucleotides for diagnostics and nanotechnology. Here, the authors present an accurate method to measure the free energy of a given DNA structure at specific temperature and
Externí odkaz:
https://doaj.org/article/70563125abd9404bb53282d9b53de0f4
Autor:
Jin H. Bae, Ruolin Liu, Eugenia Roberts, Erica Nguyen, Shervin Tabrizi, Justin Rhoades, Timothy Blewett, Kan Xiong, Gregory Gydush, Douglas Shea, Zhenyi An, Sahil Patel, Ju Cheng, Sainetra Sridhar, Mei Hong Liu, Emilie Lassen, Anne-Bine Skytte, Marta Grońska-Pęski, Jonathan E. Shoag, Gilad D. Evrony, Heather A. Parsons, Erica L. Mayer, G. Mike Makrigiorgos, Todd R. Golub, Viktor A. Adalsteinsson
Publikováno v:
Nature Genetics. 55:871-879
Detecting mutations from single DNA molecules is crucial in many fields but challenging. Next-generation sequencing (NGS) affords tremendous throughput but cannot directly sequence double-stranded DNA molecules (‘single duplexes’) to discern the
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
Nature Communications
Nature Communications
The potential of DNA as an information storage medium is rapidly growing due to advances in DNA synthesis and sequencing. However, the chemical stability of DNA challenges the complete erasure of information encoded in DNA sequences. Here, we encode
Autor:
Jin H. Bae, Douglas Shea, Todd R. Golub, Timothy Blewett, G. Mike Makrigiorgos, Erica Nguyen, Kan Xiong, Ruolin Liu, Justin Rhoades, Viktor A. Adalsteinsson
Publikováno v:
Nucleic Acids Research
Accurate DNA sequencing is crucial in biomedicine. Underlying the most accurate methods is the assumption that a mutation is true if altered bases are present on both strands of the DNA duplex. We now show that this assumption can be wrong. We establ
Autor:
Viktor A. Adalsteinsson, Shervin Tabrizi, Todd R. Golub, Blewett T, Douglas Shea, An Z, Patel S, Justin Rhoades, Ruolin Liu, Greg Gydush, Makrigiorgos Gm, Jin H. Bae, Erica Nguyen, Kan Xiong
Detecting mutations as rare as a single molecule is crucial in many fields such as cancer diagnostics and aging research but remains challenging. Third generation sequencers can read a double-stranded DNA molecule (a ‘single duplex’) in whole to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aa653026c31a9dece27b41ec91c8c1dd
https://doi.org/10.1101/2021.06.11.448110
https://doi.org/10.1101/2021.06.11.448110
Autor:
Gregory, Gydush, Erica, Nguyen, Jin H, Bae, Timothy, Blewett, Justin, Rhoades, Sarah C, Reed, Douglas, Shea, Kan, Xiong, Ruolin, Liu, Fangyan, Yu, Ka Wai, Leong, Atish D, Choudhury, Daniel G, Stover, Sara M, Tolaney, Ian E, Krop, J, Christopher Love, Heather A, Parsons, G, Mike Makrigiorgos, Todd R, Golub, Viktor A, Adalsteinsson
Publikováno v:
Nature biomedical engineering. 6(3)
Assaying for large numbers of low-frequency mutations requires sequencing at extremely high depth and accuracy. Increasing sequencing depth aids the detection of low-frequency mutations yet limits the number of loci that can be simultaneously probed.
Publikováno v:
Nucleic Acids Research
Understanding the thermodynamics of DNA motifs is important for prediction and design of probes and primers, but melt curve analyses are low-throughput and produce inaccurate results for motifs such as bulges and mismatches. Here, we developed a new,
Publikováno v:
Applied Microbiology and Biotechnology. 98:8917-8925
A major problem of long-chain fatty acid (LCFA) hydroxylation using Escherichia coli is that FadD (long-chain fatty acyl-CoA synthetase), which is necessary for exogenous LCFA transport, also initiates cellular consumption of LCFA. In this study, an