Zobrazeno 1 - 10
of 152
pro vyhledávání: '"Jim, Warwicker"'
Autor:
Shalaw Sallah, Jim Warwicker
Publikováno v:
PLoS ONE, Vol 19, Iss 11, p e0314022 (2024)
Metabolic changes during tumour development lead to acidification of the extracellular environment and a smaller increase of intracellular pH. Searches for somatic missense mutations that could reveal adaptation to altered pH have focussed on arginin
Externí odkaz:
https://doaj.org/article/73fb30cc628b4948b71128004372ee58
Publikováno v:
eLife, Vol 12 (2023)
Flaviviruses are enveloped viruses which include human pathogens that are predominantly transmitted by mosquitoes and ticks. Some, such as dengue virus, exhibit the phenomenon of antibody-dependent enhancement (ADE) of disease, making vaccine-based r
Externí odkaz:
https://doaj.org/article/0b6f1b40f7e8491f968e488d8663219f
Autor:
Rahul Khetan, Robin Curtis, Charlotte M. Deane, Johannes Thorling Hadsund, Uddipan Kar, Konrad Krawczyk, Daisuke Kuroda, Sarah A. Robinson, Pietro Sormanni, Kouhei Tsumoto, Jim Warwicker, Andrew C.R. Martin
Publikováno v:
mAbs, Vol 14, Iss 1 (2022)
Therapeutic monoclonal antibodies and their derivatives are key components of clinical pipelines in the global biopharmaceutical industry. The availability of large datasets of antibody sequences, structures, and biophysical properties is increasingl
Externí odkaz:
https://doaj.org/article/c03a214cecc447edb4481e81ade59152
Publikováno v:
Molecules, Vol 28, Iss 15, p 5753 (2023)
Many transmembrane proteins are modulated by intracellular or extracellular pH. Investigation of pH dependence generally proceeds by mutagenesis of a wide set of amino acids, guided by properties such as amino-acid conservation and structure. Predict
Externí odkaz:
https://doaj.org/article/69cbc04234624630bcf7bf2644ef3502
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
Abstract Charge is a key determinant of intrinsically disordered protein (IDP) and intrinsically disordered region (IDR) properties. IDPs and IDRs are enriched in sites of phosphorylation, which alters charge. Visualizing the degree to which phosphor
Externí odkaz:
https://doaj.org/article/4e6fab92ba564343bcfe957238eafca2
Publikováno v:
Molecules, Vol 28, Iss 8, p 3317 (2023)
Bestrophin 1 (Best1) is a chloride channel that localises to the plasma membrane of retinal pigment epithelium (RPE) cells. Mutations in the BEST1 gene are associated with a group of untreatable inherited retinal dystrophies (IRDs) called bestrophino
Externí odkaz:
https://doaj.org/article/2e884a38174043fbbad87c044cc5fddf
Autor:
Vanessa R. Lobo, Jim Warwicker
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 19, Iss , Pp 5140-5148 (2021)
Transition between receptor binding domain (RBD) up and down forms of the SARS-CoV-2 spike protein trimer is coupled to receptor binding and is one route by which variants can alter viral properties. It is becoming apparent that key roles in the tran
Externí odkaz:
https://doaj.org/article/43e7ce188deb4ca7b69c1957fdb94490
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 18, Iss , Pp 897-905 (2020)
Engineered proteins are increasingly being required to function or pass through environmental stresses for which the underlying protein has not evolved. A major example in health are antibody therapeutics, where a low pH step is used for purification
Externí odkaz:
https://doaj.org/article/07052a51e5674635b9cb0b876683f728
Autor:
Jim Warwicker
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
Since pH sensitivity has a fundamental role in biology, much effort has been committed to establishing physical models to rationalize and predict pH dependence from molecular structures. Two of the key challenges are to accurately calculate ionizable
Externí odkaz:
https://doaj.org/article/2a6e540a7e1049c2b6585944cb54f621
Publikováno v:
BMC Biotechnology, Vol 19, Iss 1, Pp 1-10 (2019)
Abstract Background Protein solubility characteristics are important determinants of success for recombinant proteins in relation to expression, purification, storage and administration. Escherichia coli offers a cost-efficient expression system. An
Externí odkaz:
https://doaj.org/article/9ff178f849e442ab825caca2d642df7b