Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA

Autor: Jill Deleu, Kathleen Schoofs, Anneleen Decock, Kimberly Verniers, Sofie Roelandt, Angie Denolf, Joke Verreth, Bram De Wilde, Tom Van Maerken, Katleen De Preter, Jo Vandesompele

Publikováno v: Human Genomics, Vol 16, Iss 1, Pp 1-14 (2022)

Abstract Background Blood plasma, one of the most studied liquid biopsies, contains various molecules that have biomarker potential for cancer detection, including cell-free DNA (cfDNA) and cell-free RNA (cfRNA). As the vast majority of cell-free nuc

Externí odkaz: https://doaj.org/article/ef68bffeabae4ba1a11a505e8f590a7c

Whole transcriptome profiling of liquid biopsies from tumour xenografted mouse models enables specific monitoring of tumour-derived extracellular RNA

Autor: Vanessa Vermeirssen, Jill Deleu, Annelien Morlion, Celine Everaert, Jilke De Wilde, Jasper Anckaert, Kaat Durinck, Justine Nuytens, Muhammad Rishfi, Frank Speleman, Hanne Van Droogenbroeck, Kimberly Verniers, Maria Francesca Baietti, Maarten Albersen, Eleonora Leucci, Edward Post, Myron G Best, Tom Van Maerken, Bram De Wilde, Jo Vandesompele, Anneleen Decock

Publikováno v: Vermeirssen, V, Deleu, J, Morlion, A, Everaert, C, De Wilde, J, Anckaert, J, Durinck, K, Nuytens, J, Rishfi, M, Speleman, F, Van Droogenbroeck, H, Verniers, K, Baietti, M F, Albersen, M, Leucci, E, Post, E, Best, M G, Van Maerken, T, De Wilde, B, Vandesompele, J & Decock, A 2022, ' Whole transcriptome profiling of liquid biopsies from tumour xenografted mouse models enables specific monitoring of tumour-derived extracellular RNA ', NAR Cancer, vol. 4, no. 4, zcac037 . https://doi.org/10.1093/narcan/zcac037
NAR CANCER
Ghent University Academic Bibliography
NAR Cancer, 4(4):zcac037

While cell-free DNA (cfDNA) is widely being investigated, free circulating RNA (extracellular RNA, exRNA) has the potential to improve cancer therapy response monitoring and detection due to its dynamic nature. However, it remains unclear in which bl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ee3e449884e4fcebdc0bb3a8278f854
https://lirias.kuleuven.be/handle/20.500.12942/708090

Performance evaluation of RNA purification kits and blood collection tubes in the Extracellular RNA Quality Control (exRNAQC) study

Autor: Bert Dhondt, Gary P. Schroth, Celine Everaert, Avila Cobos F, Jasper Anckaert, Nurten Yigit, Jill Deleu, Eva Hulstaert, Van Maerken T, Vanden Eynde E, Piofczyk T, Scott Kuersten, Annelien Morlion, De Wever O, Anneleen Decock, De Wilde J, Kathleen Schoofs, Pieter Mestdagh, Kimberly Verniers, Thibaut D’huyvetter, An Hendrix, Justine Nuytens, Philippron A, Hetty Helsmoortel, Jo Vandesompele, Van Paemel R, Nele Nijs, Carolina Fierro

The use of blood-based extracellular RNA (cell-free RNA; exRNA) as clinical biomarker requires the implementation of a validated procedure for sample collection, processing, and profiling. So far, no study has systematically addressed the pre-analyti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::33c22152e1f08a0a56865339c2c14a4c
https://doi.org/10.1101/2021.05.11.442610

Abstract B49: Substantial performance differences among RNA purification kits and blood collection tubes in the Extracellular RNA Quality Control study—important considerations for liquid biopsies

Autor: Francisco Avila Cobos, Jill Deleu, Ruben Van Paemel, Jasper Anckaert, Justine Nuytens, An Hendrix, Kathleen Schoofs, Nurten Yigit, Olivier De Wever, Eveline Vanden Eynde, Hetty Helsmoortel, Celine Everaert, Eva Hulstaert, Jo Vandesompele, Annelien Morlion, Annouck Philippron, Gary P. Schroth, Scott Kuersten, Bert Dhondt, Anneleen Decock, Pieter Mestdagh, Nele Nijs, Kimberly Verniers, Carolina Fierro

Publikováno v: Clinical Cancer Research. 26:B49-B49

Cancer biomarker studies require procedures that provide accurate and precise test results with high analytical sensitivity. Consequently, the growing use of extracellular RNA from human biofluids as clinically relevant biomarker requires the impleme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::731be5cdcdf066d65146758942008732
https://doi.org/10.1158/1557-3265.liqbiop20-b49

Janus-dendrimer supramolecular structures as delivery agents for small molecules, peptides and proteins

Autor: Luis M. Bimbo, Sami Nummelin, Markus Selin, Jarmo Ropponen, Sacha Legrand, Jill Deleu, Ville Liljeström, Antti Nykänen, Jari Koivisto, Manu Lahtinen, Jouni Hirvonen, Leena Peltonen, Tapani Viitala, Jani Seitsonen, Mauri Kostiainen

Publikováno v: University of Strathclyde
CIÊNCIAVITAE
CRS Annual Meeting and Exposition

Janus-dendrimers are synthetic amphiphiles formed by linking two chemically distinct hydrophilic and hydrophobic dendrons by their core, which can self-assemble as vesicles (dendrimersomes) or fibres in aqueous solutions, as well as in biological med

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::051c821c43b15db18216948927d8edc7
https://pureportal.strath.ac.uk/en/publications/9699fc83-8d7b-4fdd-979c-7483378395bc