CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion

Autor: Haiwei Mou, Jordan L. Smith, Lingtao Peng, Hao Yin, Jill Moore, Xiao-Ou Zhang, Chun-Qing Song, Ankur Sheel, Qiongqiong Wu, Deniz M. Ozata, Yingxiang Li, Daniel G. Anderson, Charles P. Emerson, Erik J. Sontheimer, Melissa J. Moore, Zhiping Weng, Wen Xue

Publikováno v: Genome Biology, Vol 18, Iss 1, Pp 1-8 (2017)

Abstract CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated

Externí odkaz: https://doaj.org/article/eb5d5328d15c438ebd69f1e862c787e0

Annotation of chromatin states in 66 complete mouse epigenomes during development

Autor: Michael J. Purcaro, Henry Pratt, Junko Tsuji, Zhiping Weng, Kaili Fan, Jill Moore, Arjan van der Velde

Publikováno v: Communications Biology, Vol 4, Iss 1, Pp 1-15 (2021)
Communications Biology

The morphologically and functionally distinct cell types of a multicellular organism are maintained by their unique epigenomes and gene expression programs. Phase III of the ENCODE Project profiled 66 mouse epigenomes across twelve tissues at daily i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06b2a9e4635382c7d77437fda977691e
https://doaj.org/article/072d42cc11dc4315b1f415caf2caf81e

Derivation and Initial Validation of Clinical Phenotypes of Children Presenting with Concussion Acutely in the Emergency Department: Latent Class Analysis of a Multi-Center, Prospective Cohort, Observational Study

Autor: Keith Owen, Yeates, Kenneth, Tang, Nick, Barrowman, Stephen B, Freedman, Jocelyn, Gravel, Isabelle, Gagnon, Gurinder, Sangha, Kathy, Boutis, Darcy, Beer, William, Craig, Emma, Burns, Ken J, Farion, Angelo, Mikrogianakis, Karen, Barlow, Alexander S, Dubrovsky, Willem, Meeuwisse, Gerard, Gioia, William P, Meehan, Miriam H, Beauchamp, Yael, Kamil, Anne M, Grool, Blaine, Hoshizaki, Peter, Anderson, Brian L, Brooks, Michael, Vassilyadi, Terry, Klassen, Michelle, Keightley, Lawrence, Richer, Carol, DeMatteo, Martin H, Osmond, Roger, Zemek, Jill, Moore

Publikováno v: Paediatrics Publications

The identification of clinical phenotypes may help parse the substantial heterogeneity that characterizes children with concussion. This study used latent class analysis (LCA) to identify discernible phenotypes among children with acute concussion an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b361ec2fc334e7c1a50a161b50b9fa2c
https://doi.org/10.1089/neu.2018.6009

A catalog of transcription start sites across 115 human tissue and cell types

Autor: Xiao-Ou Zhang, Elhajjajy Si, Zhiping Weng, Jill Moore, Fairlie Reese, Kaili Fan, Ali Mortazavi

Accurate transcription start site (TSS) annotations are essential for understanding transcriptional regulation and its role in human disease. Gene collections such as GENCODE contain annotations for tens of thousands of TSSs, but not all of these ann

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a364b515b2114ee876775a4513d6f292
https://doi.org/10.1101/2021.05.12.443890

Expanded encyclopaedias of DNA elements in the human and mouse genomes

Autor: Carrie A. Davis, Charles B. Epstein, Eric L. Van Nostrand, Valentina Snetkova, Michael J. Purcaro, Manolis Kellis, Yupeng He, Henry Pratt, John A. Stamatoyannopoulos, Ali Mortazavi, Xintao Wei, Michael Snyder, Jialing Zhang, Job Dekker, Anshul Kundaje, Shaimae I. Elhajjajy, Gene W. Yeo, Jessica Halow, Peggy J. Farnham, Kevin P. White, Xiaofeng Wang, Eric M. Mendenhall, Diane E. Dickel, John L. Rinn, Thomas R. Gingeras, Yin Shen, Juan Carlos Rivera-Mulia, David U. Gorkin, William Stafford Noble, Rajinder Kaul, David M. Gilbert, Jill Moore, Xiao-Ou Zhang, Peter Freese, Bing Ren, Joseph R. Ecker, Eric Lécuyer, Christopher B. Burge, Ross C. Hardison, Jing Zhang, Zhiping Weng, Richard M. Myers, Robert J. Klein, Brian A. Williams, Alexander Dobin, Alec Victorsen, Noam Shoresh, Joel Rozowsky, Jack Huey, Bradley E. Bernstein, Mark Mackiewicz, Roderic Guigó, Axel Visel, Brenton R. Graveley, J. Michael Cherry, Trupti Kawli, Mark Gerstein, Cheryl A. Keller, Barbara J. Wold, Jessika Adrian, Len A. Pennacchio, Florencia Pauli-Behn, Surya B. Chhetri

Publikováno v: Moore, Jill E; Purcaro, Michael J; Pratt, Henry E; Epstein, Charles B; Shoresh, Noam; Adrian, Jessika; Kawli, Trupti; Davis, Carrie A; Dobin, Alexander; Kaul, Rajinder; Halow, Jessica; Van Nostrand, Eric L; Freese, Peter; Gorkin, David U; Shen, Yin; He, Yupeng; Mackiewicz, Mark; Pauli-Behn, Florencia; Williams, Brian A; Mortazavi, Ali; ... (2020). Expanded encyclopaedias of DNA elements in the human and mouse genomes. Nature, 583(7818), pp. 699-710. Springer Nature 10.1038/s41586-020-2493-4
Nature
Nature, vol. 583, no. 7818, pp. 699-710

The human and mouse genomes contain instructions that specify RNAs and proteins and govern the timing, magnitude, and cellular context of their production. To better delineate these elements, phase III of the Encyclopedia of DNA Elements (ENCODE) Pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c895e469a429f18f75d20f5b0e64d2e
https://boris.unibe.ch/146110/1/s41586-020-2493-4.pdf