Zobrazeno 1 - 10
of 533
pro vyhledávání: '"Jiahuai, Han"'
Publikováno v:
Cell Death Discovery, Vol 10, Iss 1, Pp 1-11 (2024)
Abstract Caspase-8 (Casp8) serves as an initiator of apoptosis or a suppressor of necroptosis in context-dependent manner. Members of the p90 RSK family can phosphorylate caspase-8 at threonine-265 (T265), which can inactivate caspase-8 for bypassing
Externí odkaz:
https://doaj.org/article/1aad7308479c44b49ec1ab056d7c7e5d
Publikováno v:
Smart Medicine, Vol 3, Iss 3, Pp n/a-n/a (2024)
Abstract Peptide spectrum matching is the process of linking mass spectrometry data with peptide sequences. An experimental spectrum can match thousands of candidate peptides with variable modifications leading to an exponential increase in candidate
Externí odkaz:
https://doaj.org/article/105cbf1cc1244fab975b97c3fa297ef7
Publikováno v:
Communications Biology, Vol 6, Iss 1, Pp 1-12 (2023)
Abstract CRISPR-mediated knock-in (KI) technology opens a new era of fluorescent-protein labeling in zebrafish, a preferred model organism for in vivo imaging. We described here an optimized zebrafish gene-tagging strategy, which enables easy and hig
Externí odkaz:
https://doaj.org/article/672a7a2ac0bc4f8a99af6382a4e5e321
Autor:
Xiurong Wu, Jiahuai Han
Publikováno v:
STAR Protocols, Vol 4, Iss 4, Pp 102581- (2023)
Summary: Inflammasomes are multimeric protein complexes that have crucial functions in innate immunity. Here, we present a protocol to reconstitute the PELO-driven assembly of NAIP5-NLRC4 inflammasome in vitro. We describe steps for expression and pu
Externí odkaz:
https://doaj.org/article/fc825dd093b44da7b6244437ad2cf46b
Autor:
Jianfeng Wu, Kang Yang, Shaowei Cai, Xiaohan Zhang, Lichen Hu, Fanjia Lin, Su-qin Wu, Changchun Xiao, Wen-Hsien Liu, Jiahuai Han
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-20 (2022)
Plasma cells are terminally differentiated B cells that are specialized for antibody secretion. Authors show here that genomic deletion of the p38α mitogen activated protein kinase specifically in the B cell lineage leads to diminished plasma cell d
Externí odkaz:
https://doaj.org/article/7eacb188fa0445489b0d57415f695ccf
Autor:
Qingzu He, Chuan-Qi Zhong, Xiang Li, Huan Guo, Yiming Li, Mingxuan Gao, Rongshan Yu, Xianming Liu, Fangfei Zhang, Donghui Guo, Fangfu Ye, Tiannan Guo, Jianwei Shuai, Jiahuai Han
Publikováno v:
Research, Vol 6 (2023)
Data-independent acquisition (DIA) technology for protein identification from mass spectrometry and related algorithms is developing rapidly. The spectrum-centric analysis of DIA data without the use of spectra library from data-dependent acquisition
Externí odkaz:
https://doaj.org/article/d0be26ca488f4babb19b9c7a9d40a104
Autor:
Mingxuan Gao, Wenxian Yang, Chenxin Li, Yuqing Chang, Yachen Liu, Qingzu He, Chuan-Qi Zhong, Jianwei Shuai, Rongshan Yu, Jiahuai Han
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-10 (2021)
Gao et al. report DreamDIAXMBD, a deep learning-based tool, that can extract and score chromatogram features, improving the performance of peptide-centric DIA data analysis. In contrast to the existing tools, DreamDIAXMBD demonstrates higher numbers
Externí odkaz:
https://doaj.org/article/8075eaca0e5b4dbbb75082ecd8e3e7ad
Autor:
Huabin Ma, Zhoujie Zhu, Huaipeng Lin, Shanshan Wang, Peipei Zhang, Yanguo Li, Long Li, Jinling Wang, Yufen Zhao, Jiahuai Han
Publikováno v:
Cell Discovery, Vol 7, Iss 1, Pp 1-4 (2021)
Externí odkaz:
https://doaj.org/article/be6f14db5392460eb1b17ee2a8d756b4
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101517- (2022)
Summary: Though phospho-receptor-interacting protein 3 (RIP3 or RIPK3) antibodies are used in western blot, immunostaining of murine phospho-RIPK3 is challenging. Here, we verify and describe a detailed protocol for immunofluorescent detection of pho
Externí odkaz:
https://doaj.org/article/0959ef27378b4d349b50cbc2e0347873
Autor:
Yating Lin, Shangze Wu, Xu Xiao, Jingbo Zhao, Minshu Wang, Haojun Li, Kejia Wang, Minwei Zhang, Frank Zheng, Wenxian Yang, Lei Zhang, Jiahuai Han, Rongshan Yu
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101587- (2022)
Summary: Computational protocols for cell type deconvolution from bulk RNA-seq data have been used to understand cellular heterogeneity in disease-related samples, but their performance can be impacted by batch effect among datasets. Here, we present
Externí odkaz:
https://doaj.org/article/fd5b1ca784704befac6881ebfd615109