Tailoring FPOX enzymes for enhanced stability and expanded substrate recognition

Autor: Hajar Estiri, Shapla Bhattacharya, Jhon Alexander Rodriguez Buitrago, Rossella Castagna, Linda Legzdiņa, Giorgia Casucci, Andrea Ricci, Emilio Parisini, Alfonso Gautieri

Publikováno v: Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)

Abstract Fructosyl peptide oxidases (FPOX) are deglycating enzymes that find application as key enzymatic components in diabetes monitoring devices. Indeed, their use with blood samples can provide a measurement of the concentration of glycated hemog

Externí odkaz: https://doaj.org/article/836253ca26534ec0a71e318ae550b427

CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering

Autor: Wulf Blankenfeldt, Jhon Alexander Rodriguez Buitrago, Agustina Vila, Bastian Nicolai, Patrick Schrepfer, Paula Bracco, Dick B. Janssen, Hein J. Wijma, Thomas Klünemann, Anett Schallmey

Publikováno v: ChemBioChem : a European journal of chemical biology 22(6), 1099-1110 (2020). doi:10.1002/cbic.202000735
ChemBioChem, 22(6), 1099-1110. WILEY-V C H VERLAG GMBH
Chembiochem : a European journal of chemical biology Bd. 22, H. 6 (2021), S. 1099-1110--Chembiochem--1439-4227--1439-7633
Chembiochem
Chembiochem : a European journal of chemical biology
Germany

CYP154C5 from Nocardia farcinica is a P450 monooxygenase able to hydroxylate a range of steroids with high regio‐ and stereoselectivity at the 16α‐position. Using protein engineering and substrate modifications based on the crystal structure of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66ff3451b4d6411a2686e91616cfe5e0
https://publications.rwth-aachen.de/record/810303