Zobrazeno 1 - 10
of 55
pro vyhledávání: '"Jeung-Hoi Ha"'
Autor:
Mohammad Ahmad, Jeung-Hoi Ha, Lauren A. Mayse, Maria F. Presti, Aaron J. Wolfe, Kelsey J. Moody, Stewart N. Loh, Liviu Movileanu
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Sensitive and accurate approaches for protein detection have many potential applications. Here the authors show how engineered protein nanopore sensors, consisting of a monobody fused to a single-polypeptide nanopore, can be used for highly specific
Externí odkaz:
https://doaj.org/article/1f2b1191daee43b2bc50ec7144ff89b9
Enhancing response of a protein conformational switch by using two disordered ligand binding domains
Publikováno v:
Frontiers in Molecular Biosciences, Vol 10 (2023)
Introduction: Protein conformational switches are often constructed by fusing an input domain, which recognizes a target ligand, to an output domain that establishes a biological response. Prior designs have employed binding-induced folding of the in
Externí odkaz:
https://doaj.org/article/35e72e9ba6864d34b5f29038a32f3c6c
Autor:
Jing Zhao, Alan Blayney, Xiaorong Liu, Lauren Gandy, Weihua Jin, Lufeng Yan, Jeung-Hoi Ha, Ashley J. Canning, Michael Connelly, Chao Yang, Xinyue Liu, Yuanyuan Xiao, Michael S. Cosgrove, Sozanne R. Solmaz, Yingkai Zhang, David Ban, Jianhan Chen, Stewart N. Loh, Chunyu Wang
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
Epigallocatechin gallate (EGCG) is a catechin flavonoid which induces apoptosis in cancerous cells, but the underlying molecular mechanisms remain poorly understood. Here authors use an interdisciplinary approach to show a direct interaction between
Externí odkaz:
https://doaj.org/article/bfe12496e2674532a1bf41a125983054
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
A large percentage of transcription factors require zinc to bind DNA. In this review, we discuss what makes p53 unique among zinc-dependent transcription factors. The conformation of p53 is unusually malleable: p53 binds zinc extremely tightly when f
Externí odkaz:
https://doaj.org/article/1fbf4fa9b2c742928e4cf2866141f47d
Autor:
Adam R Blanden, Xin Yu, Alan J Blayney, Christopher Demas, Jeung-Hoi Ha, Yue Liu, Tracy Withers, Darren R Carpizo, Stewart N Loh
Publikováno v:
eLife, Vol 9 (2020)
Missense mutations in the p53 DNA-binding domain (DBD) contribute to half of new cancer cases annually. Here we present a thermodynamic model that quantifies and links the major pathways by which mutations inactivate p53. We find that DBD possesses t
Externí odkaz:
https://doaj.org/article/414b92a82c8d4ddc9d7a0884046cf056
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
The rational optimization of response times of protein conformational switches is a major challenge for biomolecular switch design. Here the authors present a generally applicable computational design strategy that in combination with biophysical exp
Externí odkaz:
https://doaj.org/article/94220e635e4c46a6835d838c064d7ecc
Autor:
Jeung-Hoi Ha1, Yikang Xu2, Sekhon, Harsimranjit1, Wenhan Zhao2, Wilkens, Stephan1, Ren, Dacheng2,3,4 dren@syr.edu, Loh, Stewart N.1 lohs@upstate.edu
Publikováno v:
Journal of Biological Chemistry. May2024, Vol. 300 Issue 5, p1-13. 13p.
Autor:
Harsimranjit Sekhon, Jeung-Hoi Ha, Maria F. Presti, Spencer B. Procopio, Paige O. Mirsky, Anna M. John, Stewart N. Loh
Publikováno v:
bioRxiv
A grand challenge in biosensor design is to develop a single molecule, fluorescent protein-based platform that can be easily adapted to recognize targets of choice. Conceptually, this can be achieved by fusing a small, antibody-like binding domain to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01df9e3919de7435abd48ac624f757f0
https://doi.org/10.1101/2023.03.28.534597
https://doi.org/10.1101/2023.03.28.534597
Publikováno v:
ACS Sensors. 7:263-271
Protein conformational switches are widely used in biosensing. They are often composed of an input domain (which binds a target ligand) fused to an output domain (which generates an optical readout). A central challenge in designing such switches is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e99ef81e6e3451c774af570bce00691b
https://doi.org/10.1021/acssensors.1c02239
https://doi.org/10.1021/acssensors.1c02239
Protein detection and biomarker profiling have implications in basic research and molecular diagnostics. Substantial progress has been made in protein analytics using nanopores and the resistive-pulse technique. Yet, a long-standing challenge is impl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::422f7f24d4c464c49ee1713c0f7635ec