A unifying structural and functional model of the coronavirus replication organelle: Tracking down RNA synthesis.

Autor: Eric J Snijder, Ronald W A L Limpens, Adriaan H de Wilde, Anja W M de Jong, Jessika C Zevenhoven-Dobbe, Helena J Maier, Frank F G A Faas, Abraham J Koster, Montserrat Bárcena

Publikováno v: PLoS Biology, Vol 18, Iss 6, p e3000715 (2020)

Zoonotic coronavirus (CoV) infections, such as those responsible for the current severe acute respiratory syndrome-CoV 2 (SARS-CoV-2) pandemic, cause grave international public health concern. In infected cells, the CoV RNA-synthesizing machinery ass

Externí odkaz: https://doaj.org/article/57b78e91f3444cc5af1613ba09d4998a

Structure-function analysis of the nsp14 N7-guanine methyltransferase reveals an essential role in Betacoronavirus replication

Autor: Volker Thiel, Jessika C. Zevenhoven-Dobbe, Clara C. Posthuma, Priscila El Kazzi, Eric J. Snijder, Alice Decombe, François Ferron, Natacha S. Ogando, Etienne Decroly, Bruno Canard, Brenda w. Bontes

Publikováno v: Proceedings of the National Academy of Sciences, 118(49). NATL ACAD SCIENCES
Ogando, Natacha S; El Kazzi, Priscila; Zevenhoven-Dobbe, Jessika C; Bontes, Brenda W; Decombe, Alice; Posthuma, Clara C; Thiel, Volker; Canard, Bruno; Ferron, François; Decroly, Etienne; Snijder, Eric J (2021). Structure-function analysis of the nsp14 N7-guanine methyltransferase reveals an essential role in Betacoronavirus replication. Proceedings of the National Academy of Sciences of the United States of America-PNAS, 118(49) National Academy of Sciences NAS 10.1073/pnas.2108709118
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2021, ⟨10.1101/2021.05.17.444407⟩

As coronaviruses (CoVs) replicate in the host cell cytoplasm, they rely on their own capping machinery to ensure the efficient translation of their mRNAs, protect them from degradation by cellular 5’ exoribonucleases, and escape innate immune sensi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::156d64145c316b53403328c37d9acc11
https://hal-amu.archives-ouvertes.fr/hal-03426119/file/2021.05.17.444407.full.pdf

6′,6′-Difluoro-aristeromycin is a potent inhibitor of MERS-coronavirus replication

Autor: Sushil Kumar Tripathi, Jessika C. Zevenhoven-Dobbe, Clara C. Posthuma, Lak Shin Jeong, Natacha S. Ogando, Hyuk Woo Lee, Dnyandev B. Jarhad, Eric J. Snijder

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has highlighted the lack of treatments to combat infections with human or (potentially) zoonotic CoVs. Thus, it is critical to develop and evaluate antiviral compounds that eit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7ba5ae0fa739b3e7590d5e4322c0c909
https://doi.org/10.1101/2021.05.20.445077

A molecular pore spans the double membrane of the coronavirus replication organelle

Autor: Jessika C. Zevenhoven-Dobbe, Georg Wolff, Ronald W. A. L. Limpens, Shawn Q. Zheng, Roman I. Koning, Ulrike Laugks, Abraham J. Koster, Montserrat Bárcena, David A. Agard, Eric J. Snijder, Anja W. M. de Jong, Kay Grünewald

Publikováno v: Science
Science, 369(6509), 1395-+. AMER ASSOC ADVANCEMENT SCIENCE

Coronavirus genome replication is associated with virus-induced cytosolic double-membrane vesicles, which may provide a tailored microenvironment for viral RNA synthesis in the infected cell. However, it is unclear how newly synthesized genomes and m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a89141df0fc0f1f7ef2060b0d9892405
https://hdl.handle.net/1887/3184106

The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2

Autor: Jessika C. Zevenhoven-Dobbe, Clara C. Posthuma, Peter J. Bredenbeek, Eric J. Snijder, Yvonne van der Meer, Natacha S. Ogando

Publikováno v: Journal of Virology
Journal of Virology, 94(23). AMER SOC MICROBIOLOGY

The bifunctional nsp14 subunit of the coronavirus replicase contains 3′-to-5′ exoribonuclease (ExoN) and guanine-N7-methyltransferase domains. For the betacoronaviruses MHV and SARS-CoV, ExoN was reported to promote the fidelity of genome replica

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5714becdffaa80b5d78867710826a3d