Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids.

Autor: Thorsten Hugel, Jens Michaelis, Craig L Hetherington, Paul J Jardine, Shelley Grimes, Jessica M Walter, Wayne Falk, Dwight L Anderson, Carlos Bustamante

Publikováno v: PLoS Biology, Vol 5, Iss 3, p e59 (2007)

The bacteriophage phi29 generates large forces to compact its double-stranded DNA genome into a protein capsid by means of a portal motor complex. Several mechanical models for the generation of these high forces by the motor complex predict coupling

Externí odkaz: https://doaj.org/article/c07a6fcd72fd47bdaa6c3424c0845db8

Method for Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas9

Autor: Kristy Michelle Hawkins, Darren Platt, Max G. Schubert, Sunil S. Chandran, Andrew A. Horwitz, Jack D. Newman, Tina Mahatdejkul-Meadows, Jessica M. Walter, Stephanie H. Kung, Aaron D. Hernday, Wayne Szeto

Publikováno v: Methods in Molecular Biology ISBN: 9781493997350

CRISPR-Cas has proven to be a powerful tool for precision genetic engineering in a variety of difficult genetic systems. In the highly tractable yeast S. cerevisiae, CRISPR-Cas can be used to conduct multiple engineering steps in parallel, allowing f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ad30205bac9a685c020b058739f269cf
https://doi.org/10.1007/978-1-4939-9736-7_3

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

Autor: Aaron D. Hernday, Sunil S. Chandran, Stephanie H. Kung, Wayne Szeto, Jessica M. Walter, Max G. Schubert, Tina Mahatdejkul-Meadows, Kristy Michelle Hawkins, Darren Platt, Jack D. Newman, Andrew A. Horwitz

Publikováno v: Horwitz, AA; Walter, JM; Schubert, MG; Kung, SH; Hawkins, K; Platt, DM; et al.(2015). Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas. Cell Systems, 1(1), 88-96. doi: 10.1016/j.cels.2015.02.001. UC Merced: Retrieved from: http://www.escholarship.org/uc/item/01d6s8m1
Cell systems, vol 1, iss 1

© 2015 The Authors. Summary CRISPR-Cas genome engineering in yeast has relied on preparation of complex expression plasmids for multiplexed gene knockouts and point mutations. Here we show that co-transformation of a single linearized plasmid with m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d27ed41b04db0f82fc173913678fa9e8
https://doi.org/10.1016/j.cels.2015.02.001

Characterization of acellular dermal matrices (ADMs) prepared by two different methods

Autor: Robert J. Walter, Hernan M. Reyes, Jessica M. Walter, Marella Hanumadass, Takayoshi Matsuda

Publikováno v: Burns. 24:104-113

The efficacy of acellular dermal matrix (ADM) in the treatment of full-thickness skin injuries as a dermal substitute depends on its low antigenicity, capacity for rapid vascularization, and stability as a dermal template. These properties will be de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58b24ba5b0e134368f46a4b0545a7a65
https://doi.org/10.1016/s0305-4179(97)00110-1

Use of Evidence-Based Practice Among Athletic Training Educators, Clinicians, and Students, Part 2: Attitudes, Beliefs, Accessibility, and Barriers

Autor: Eric J. Newton, Cailee W. McCarty, Jessica M. Walter, Bonnie L. Van Lunen, Dorice A. Hankemeier

Context: Successful implementation of evidence-based practice (EBP) within athletic training is contingent upon understanding the attitudes and beliefs and perceived barriers toward EBP as well as the accessibility to EBP resources of athletic traini

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c26a937e64cede454b975749bb3f2ce4
https://europepmc.org/articles/PMC3655754/