CRISPR Interference of a Clonally Variant GC-Rich Noncoding RNA Family Leads to General Repression of var Genes in Plasmodium falciparum

Autor: Anna Barcons-Simon, Carlos Cordon-Obras, Julien Guizetti, Jessica M. Bryant, Artur Scherf

Publikováno v: mBio, Vol 11, Iss 1 (2020)

ABSTRACT The human malaria parasite Plasmodium falciparum uses mutually exclusive expression of the PfEMP1-encoding var gene family to evade the host immune system. Despite progress in the molecular understanding of the default silencing mechanism, t

Externí odkaz: https://doaj.org/article/03d722d0147f412f860ff784e5a0530e

CRISPR/Cas9 Genome Editing Reveals That the Intron Is Not Essential for var2csa Gene Activation or Silencing in Plasmodium falciparum

Autor: Jessica M. Bryant, Clément Regnault, Christine Scheidig-Benatar, Sebastian Baumgarten, Julien Guizetti, Artur Scherf

Publikováno v: mBio, Vol 8, Iss 4 (2017)

ABSTRACT Plasmodium falciparum relies on monoallelic expression of 1 of 60 var virulence genes for antigenic variation and host immune evasion. Each var gene contains a conserved intron which has been implicated in previous studies in both activation

Externí odkaz: https://doaj.org/article/91d89e87606c4bb78714e1eb9743529d

Exploring the virulence gene interactome with CRISPR/dCas9 in the human malaria parasite

Autor: Jessica M. Bryant, Aurélie Claes, Sebastian Baumgarten, Artur Scherf, Damarys Loew, Ameya Sinha, Peter R. Preiser, Peter C. Dedon, Florent Dingli

Publikováno v: Molecular Systems Biology
Molecular Systems Biology, EMBO Press, 2020, 16 (8), pp.e9569. ⟨10.15252/msb.20209569⟩
Molecular Systems Biology, 2020, 16 (8), pp.e9569. ⟨10.15252/msb.20209569⟩
Molecular Systems Biology, Vol 16, Iss 8, Pp n/a-n/a (2020)

Mutually exclusive expression of the var multigene family is key to immune evasion and pathogenesis in Plasmodium falciparum, but few factors have been shown to play a direct role. We adapted a CRISPR‐based proteomics approach to identify novel fac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e1ae3aa39bdacdea9109b3ba2305965
https://hal.archives-ouvertes.fr/hal-03033253/document

Transcriptome-wide dynamics of extensive m6A mRNA methylation during Plasmodium falciparum blood-stage development

Autor: Jessica M. Bryant, Ameya Sinha, Thibaud Reyser, Peter C. Dedon, Peter R. Preiser, Artur Scherf, Sebastian Baumgarten

Publikováno v: Nature Microbiology
Nature Microbiology, 2019, ⟨10.1038/s41564-019-0521-7⟩
Nature Microbiology, Nature Publishing Group, 2019, ⟨10.1038/s41564-019-0521-7⟩

International audience; Malaria pathogenesis results from the asexual replication of Plasmodium falciparum within human red blood cells, which relies on a precisely timed cascade of gene expression over a 48-h life cycle. Although substantial post-tr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e25297d8373179d397fbdb4706a3031
https://hal.science/hal-02321824

Transcriptome-wide dynamics of extensive m

Autor: Sebastian, Baumgarten, Jessica M, Bryant, Ameya, Sinha, Thibaud, Reyser, Peter R, Preiser, Peter C, Dedon, Artur, Scherf

Publikováno v: Nat Microbiol

Malaria pathogenesis results from the asexual replication of Plasmodium falciparum within human red blood cells, which relies on a precisely timed cascade of gene expression over a 48-h life cycle. Although substantial post-transcriptional regulation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f4bde751316d48b990c8402fd5ff4a19
https://pubmed.ncbi.nlm.nih.gov/31384004