Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Jerzy Olejnik"'
Autor:
Steven A. Benner, Fei Chen, Nilesh B. Karalkar, Evan R. Guggenheim, Daniel Hutter, Jerzy Olejnik, Steven Gordon, Visa Visalakshi, Myong-Jung Kim, Nicole A. Leal
Publikováno v:
Nucleosides, Nucleotides & Nucleic Acids. 29:879-895
Nucleoside triphosphates having a 3'-ONH₂ blocking group have been prepared with and without fluorescent tags on their nucleobases. DNA polymerases were identified that accepted these, adding a single nucleotide to the 3'-end of a primer in a templ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0946e2b521097412718e94a43f0ec310
https://doi.org/10.1080/15257770.2010.536191
https://doi.org/10.1080/15257770.2010.536191
Publikováno v:
Analytical Biochemistry. 326:25-32
A highly efficient method for the introduction of fluorophores and other markers at the N terminus of proteins produced in a cell-free extract has been developed. The method utilizes an amber (CUA) initiator suppressor tRNA chemically aminoacylated w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d2ef1d951529e103d9150d704ecc124
https://doi.org/10.1016/j.ab.2003.11.002
https://doi.org/10.1016/j.ab.2003.11.002
Publikováno v:
Analytical Biochemistry. 320:55-65
Integration of DNA isolation, amplification, and sequencing can be achieved by the use of polymerase colonies (polonies) and cycles of fluorescent dNTP incorporation. In this paper, we present four advances that bring us closer to sequencing genomes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6d4167e99100d434a195efc8bca9eaf
https://doi.org/10.1016/s0003-2697(03)00291-4
https://doi.org/10.1016/s0003-2697(03)00291-4
Autor:
Jerzy Olejnik, Kenneth J. Rothschild, Franz Hillenkamp, Stefan Berkenkamp, Hans-Christian Lüdemann, Edyta Krzymanska-Olejnik
Publikováno v:
Nucleic Acids Research. 27:4626-4631
The synthesis and characterization of photocleavable peptide-DNA conjugates is described along with their use as photocleavable mass marker (PCMM) hybridization probes for the detection of target DNA sequences by matrix-assisted laser desorption/ioni
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98d6a4fb8729d8c8279c8ee9f9b5a97d
https://doi.org/10.1093/nar/27.23.4626
https://doi.org/10.1093/nar/27.23.4626
Publikováno v:
Nucleic Acids Research. 26:3572-3576
We report the design and evaluation of two non-nucleosidic photocleavable aminotag phosphor-amidites. These reagents introduce a photocleavable amino group on the 5'-terminal phosphate of synthetic oligonucleotides. The 5' photocleavable amino group
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::150cca241cd87e8d7b7626e48cc721bf
https://doi.org/10.1093/nar/26.15.3572
https://doi.org/10.1093/nar/26.15.3572
Autor:
Piotr Milart, Jerzy Olejnik, Arnold Jarczewski, Bogumil Brzezinski, Eugeniusz Grech, Grzegorz Schroeder
Publikováno v:
Journal of Molecular Structure. 406:99-106
Deprotonation of `C-acids': benzoylmalononitrile (1), and 4-nitrophenylmalononitrile (2), triethoxycarbonylmethane (3), diethyl cyanomalonate (4) and ethyl 1,3-indandione-2-carboxylate (5), by 7-methyl-1,5,7-triaza-bicyclo[4.4.O]dec-5-ene (MTBD) and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8bac5f5dcc5f4741390564fa94d56bd6
https://doi.org/10.1016/s0022-2860(96)09616-0
https://doi.org/10.1016/s0022-2860(96)09616-0
Publikováno v:
Journal of Biological Chemistry. 270:29746-29751
Bacteriorhodopsin is a light-driven proton pump, which undergoes a photocycle consisting of several distinct intermediates. Previous studies have established that the M →N step of this photocycle involves a major conformational change of membrane e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::197f88e847bc7d51f0d04c8e6a6e9a30
https://doi.org/10.1074/jbc.270.50.29746
https://doi.org/10.1074/jbc.270.50.29746
Publikováno v:
ResearcherID
While the strong biotin-avidin interaction has been widely used for the detection of biomolecules, its irreversibility complicates their isolation. We report the synthesis of a photocleavable biotin derivative (PCB) which eliminates many limitations
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dce1344dc7a44cdf14f8d3fdfb976790
https://doi.org/10.1073/pnas.92.16.7590
https://doi.org/10.1073/pnas.92.16.7590
Publikováno v:
Journal of Molecular Structure. 323:71-78
3-Diethylaminomethyl-2,2′-biphenol was synthesized and studied by FT-IR and 1 H NMR spectroscopy. The compound forms a system with two hydrogen bonds which shows large proton polarizability due to collective proton motion. This result supports our
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::195fc1459287c8a33e8fe5ca674fa904
https://doi.org/10.1016/0022-2860(94)07977-3
https://doi.org/10.1016/0022-2860(94)07977-3
Publikováno v:
Journal of Molecular Structure. 300:573-592
Experimentally observed and calculated infrared (IR) continua of the homoconjugated hydrogen bonds in H 5 O + 2 or deuterium bonds in D 5 O + 2 , both of which show large polarizability, are compared. With N + H⋯N⇌N⋯H + N bonds, for instance, t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3035fa35bf26a4c344e9bcea564549b2
https://doi.org/10.1016/0022-2860(93)87048-e
https://doi.org/10.1016/0022-2860(93)87048-e