Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Jeremy A. Lombardo"'
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Existing methods for tissue dissociation are inefficient and lead to variable outcomes and biases. Here, the authors present a microfluidic platform that combines digestion, disaggregation and filtration of tissue to allow single cell analysis and RN
Externí odkaz:
https://doaj.org/article/df431d77a95e4931b3e810d6dee41623
Publikováno v:
Plast Reconstr Surg
Plastic and reconstructive surgery, vol 151, iss 1
Plastic and reconstructive surgery, vol 151, iss 1
BackgroundAdipose tissue is an easily accessible source of stem and progenitor cells that offers exciting promise as an injectable autologous therapeutic for regenerative applications. Mechanical processing is preferred over enzymatic digestion, and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc15651cac7376dfba8312dfe79ffe42
https://doi.org/10.1097/prs.0000000000009798
https://doi.org/10.1097/prs.0000000000009798
Autor:
Jeremy A. Lombardo, Nick Russell, Jiawei He, Michael J. Larson, William R. Walsh, Gregory M. Mundis, Frank Vizesi
Publikováno v:
Spine.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::419c3a386bc60b2d447e96a09c953ea7
https://doi.org/10.1097/brs.0000000000004688
https://doi.org/10.1097/brs.0000000000004688
Autor:
Jeremy A, Lombardo, Jered B, Haun
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2394
There is growing interest in breaking down tissues into the individual cellular constituents so that those cells can be identified, assayed for functional characteristics, or utilized for therapeutic purposes. A major driver is the development of sin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7981e6930be8b8b440edb2b4458843b0
https://pubmed.ncbi.nlm.nih.gov/35094323
https://pubmed.ncbi.nlm.nih.gov/35094323
Autor:
Jeremy A. Lombardo, Jered B. Haun
Publikováno v:
Methods in Molecular Biology ISBN: 9781071618103
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3add32c8f3e58daa2edec2c84fe6dcbe
https://doi.org/10.1007/978-1-0716-1811-0_6
https://doi.org/10.1007/978-1-0716-1811-0_6
Autor:
Trisha M. Westerhof, Xiaolong Qiu, Kai Kessenbrock, Marissa Pennell, Elliot E. Hui, Brian Luna, Jered B. Haun, Edward L. Nelson, Jeremy A. Lombardo, Hamad Alshetaiwi, Anita Ng
Publikováno v:
Lab on a chip, vol 18, iss 18
Tissues are increasingly being analyzed at the single cell level in order to characterize cellular diversity and identify rare cell types. Single cell analysis efforts are greatly limited, however, by the need to first break down tissues into single
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d8bd95db07fc3fabb3eb853166029eb
https://escholarship.org/uc/item/9nj068j7
https://escholarship.org/uc/item/9nj068j7
Microfluidic channel optimization to improve hydrodynamic dissociation of cell aggregates and tissue
Autor:
Edward L. Nelson, Jered B. Haun, Trisha M. Westerhof, Marissa Pennell, Jeremy A. Lombardo, Pedram P. Pourfard, Pulak Nath, Xiaolong Qiu, Jen-Huang Huang, Katrina M. Henrikson
Publikováno v:
Scientific Reports
Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
Qiu, X; Huang, J-H; Westerhof, TM; Lombardo, JA; Henrikson, KM; Pennell, M; et al.(2018). Microfluidic channel optimization to improve hydrodynamic dissociation of cell aggregates and tissue. SCIENTIFIC REPORTS, 8. doi: 10.1038/s41598-018-20931-y. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/00d4b9pr
Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
Qiu, X; Huang, J-H; Westerhof, TM; Lombardo, JA; Henrikson, KM; Pennell, M; et al.(2018). Microfluidic channel optimization to improve hydrodynamic dissociation of cell aggregates and tissue. SCIENTIFIC REPORTS, 8. doi: 10.1038/s41598-018-20931-y. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/00d4b9pr
Maximizing the speed and efficiency at which single cells can be liberated from tissues would dramatically advance cell-based diagnostics and therapies. Conventional methods involve numerous manual processing steps and long enzymatic digestion times,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ded94df54200f44e2995f17247a3b6f2
https://pubmed.ncbi.nlm.nih.gov/29426941
https://pubmed.ncbi.nlm.nih.gov/29426941