Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis

Autor: Marco Strohmeier, Volker Windeisen, Irmgard Sinning, Gwyndaf Evans, Matthew J. Rodrigues, Steven E. Ealick, Yang Zhang, Jeremiah Hanes, Ivo Tews, Tadhg P. Begley, Gabriela Guédez, Antoine Royant, Stefan Fabian Weber

Publikováno v: Nature Chemical Biology
Nature Chemical Biology, Nature Publishing Group, 2017, 13 (3), pp.290-294. ⟨10.1038/nchembio.2273⟩
Nature Chemical Biology, 2017, 13 (3), pp.290-294. ⟨10.1038/nchembio.2273⟩

International audience; Substrate channeling has emerged as a common mechanism for enzymatic intermediate transfer. A conspicuous gap in knowledge concerns the use of covalent lysine imines in the transfer of carbonyl-group-containing intermediates,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34970f0c3eeef2d7aad4fa6584bb26f0
https://hal.univ-grenoble-alpes.fr/hal-01487798/document

Role of the Sulfonium Center in Determining the Ligand Specificity of Human S-Adenosylmethionine Decarboxylase

Autor: Wesley H. Brooks, Arnold M. Mahesan, Jeremiah Hanes, Steven E. Ealick, Wayne C. Guida, Shridhar Bale

Publikováno v: Biochemistry. 48:6423-6430

S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc7c5cedfc7509194fb4a0e5be6b6b1a
https://doi.org/10.1021/bi900590m

A novel mechanism of selectivity against AZT by the human mitochondrial DNA polymerase

Autor: Jeremiah Hanes, Kenneth A. Johnson

Publikováno v: Nucleic Acids Research

Native nucleotides show a hyperbolic concentration dependence of the pre-steady-state rate of incorporation while maintaining concentration-independent amplitude due to fast, largely irreversible pyrophosphate release. The kinetics of 3'-azido-2',3'-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f44a45d64d2e14278cf5854edeb2bbd2
https://doi.org/10.1093/nar/gkm695

Single-locus enrichment without amplification for sequencing and direct detection of epigenetic modifications

Autor: Jeremiah Hanes, Regina Lam, Stephen Turner, Paul J. Hagerman, Thang Pham, Jun Yi Wang, Erick Loomis, John Eid, Evan Adams, Jun Yin

Publikováno v: Molecular genetics and genomics : MGG. 291(3)

A gene-level targeted enrichment method for direct detection of epigenetic modifications is described. The approach is demonstrated on the CGG-repeat region of the FMR1 gene, for which large repeat expansions, hitherto refractory to sequencing, are k

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e06ed3edf8c01790c413ae9ec48041c2
https://pubmed.ncbi.nlm.nih.gov/26825750

Characterization of Novel Reverse Transcriptase and Other RNA-associated Catalytic Activities by Human DNA Polymerase γ

Autor: Joy Y. Feng, Karen S. Anderson, Jeremiah Hanes, Eisuke Murakami, Harold R. Lee, Kenneth A. Johnson

Publikováno v: Journal of Biological Chemistry. 278:36403-36409

During mitochondrial DNA (mtDNA) replication, DNA/RNA heteroduplex intermediates are formed. To understand how and why ribonucleotides are involved in mtDNA replication, we have studied the novel RNA-associated activities of human mitochondrial DNA p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5b599c71385c52ecd54cd771e7020093
https://doi.org/10.1074/jbc.m306236200