Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Jeppe W. Tams"'
Publikováno v:
Journal of Molecular Neuroscience. 14:137-146
To elucidate the functional role of the second extracellular loop of human vasoactive intestinal polypeptide/pituitary adenylate cyclase-activating polypeptide (VIP/PACAP) receptor (hVPAC1R), surface expression, ligand binding, and receptor activatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cdb8559d13a0fb4b1e1caef76b880ccb
https://doi.org/10.1385/jmn:14:3:137
https://doi.org/10.1385/jmn:14:3:137
Publikováno v:
Biochemical Journal. 341:271-276
125I-Pituitary adenylate cyclase-activating polypeptide (PACAP) 1-38 is able to bind a factor in human plasma, which can be displaced by unlabelled PACAP 1-38 and PACAP 28-38 but not by the other biologically active form, PACAP 1-27. Likewise, 125I-P
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b705eccec30fde373d4ef8120868fa0b
https://doi.org/10.1042/bj3410271
https://doi.org/10.1042/bj3410271
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1432:214-221
Protein solubility is a fundamental parameter in biology and biotechnology. In the present study we have constructed and analyzed five mutants of Coprinus cinereus peroxidase (CIP) with 0, 1, 2, 4 and 6 N-glycosylation sites. All mutants contain Man(
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b435d74a27fc07bfaa8891fe6ba5a526
https://doi.org/10.1016/s0167-4838(99)00103-x
https://doi.org/10.1016/s0167-4838(99)00103-x
Autor:
Jeppe W. Tams, Karen G. Welinder
Publikováno v:
Analytical Biochemistry. 228:48-55
Horseradish peroxidase isoenzyme C (HRP) contains eight N-linked glycans composed of Man, Xyl, Fuc, and GlcNAc. These glycans were resistant to enzymatic hydrolysis by endoglycosidases peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase F, end
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c89c45bdc3225c03ce20f0e3737b2b2
https://doi.org/10.1006/abio.1995.1313
https://doi.org/10.1006/abio.1995.1313
Autor:
Karen G. Welinder, Jesper Vind, Paola Limongi, Tomas Johansson, Jeppe W. Tams, Marianne Kjalke
Publikováno v:
European Journal of Biochemistry. 227:270-276
Four conserved disulfide bonds and N-linked and O-linked glycans of extracellular fungal peroxidases have been identified from studies of a lignin and a manganese peroxidase from Trametes versicolor, and from Coprinus cinereus peroxidase (CIP) and re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d53cb49ae8e58ddf0f2598953b4ef94b
https://doi.org/10.1111/j.1432-1033.1995.tb20384.x
https://doi.org/10.1111/j.1432-1033.1995.tb20384.x
Publikováno v:
Biochemistry. 33:15425-15432
Resonance Raman (RR) spectra for the resting state ferric and the reduced ferrous forms of recombinant Coprinus cinereus peroxidase (CIP), obtained with different excitation wavelengths and in polarized light, are reported. The spectra are compared w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f000304818a5d4a3a86cf9f84f0a42fb
https://doi.org/10.1021/bi00255a024
https://doi.org/10.1021/bi00255a024
Publikováno v:
European Journal of Biochemistry. 222:909-918
Proton nuclear magnetic resonance spectroscopy has been used to characterise and compare wild-type fungal and recombinant Coprinus cinereus peroxidase (CIP) and three mutants in which Gly156 and/or Asn157 was replaced by Phe. Analysis of one- and two
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdfcdb720d4406f7b91128746d86d2c3
https://doi.org/10.1111/j.1432-1033.1994.tb18939.x
https://doi.org/10.1111/j.1432-1033.1994.tb18939.x
Publikováno v:
FEBS Letters. 412:141-143
The importance of two highly conserved cysteines in the human vasoactive intestinal peptide receptor 1 (hVIPR 1) was examined. By site-directed mutagenesis each Cys residue was converted into Ala or Ser. The mutant and wild-type genes were transfecte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b263c41dc462fcfe35ab12e1279b99c
https://doi.org/10.1016/s0014-5793(97)00714-x
https://doi.org/10.1016/s0014-5793(97)00714-x
Autor:
Jeppe W. Tams, Jens Petersen, Anders Svensson, Karen G. Welinder, Jesper Vind, Henrik Dalbøge, Sine Larsen
Publikováno v:
Journal of Molecular Biology. 232:989-991
Crystals suitable for an X-ray diffraction investigation have been obtained of recombinant Coprinus cinereus peroxidase expressed in Aspergillus oryzae . The crystals were grown by the hanging drop method with polyethylene glycol 6000 as the precipit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6d75dc8c15a852aa3636a44585efd00
https://doi.org/10.1006/jmbi.1993.1445
https://doi.org/10.1006/jmbi.1993.1445
Autor:
Karen G. Welinder, Jeppe W. Tams
Publikováno v:
Biochemical and biophysical research communications. 286(4)
The effect of glycans and surface mutations on protein unfolding induced by heat or urea has been studied. Removal of the only native high mannose type glycan in the N142P, N142T, and N142D CIP mutants reduced the lifetime to half of that of wtCIP at
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3204377c3364a27dbcd981f39e574880
https://pubmed.ncbi.nlm.nih.gov/11520054
https://pubmed.ncbi.nlm.nih.gov/11520054