Prion infections and anti-PrP antibodies trigger converging neurotoxic pathways.

Autor: Uli S Herrmann, Tiziana Sonati, Jeppe Falsig, Regina R Reimann, Paolo Dametto, Tracy O'Connor, Bei Li, Agnes Lau, Simone Hornemann, Silvia Sorce, Uli Wagner, Despina Sanoudou, Adriano Aguzzi

Publikováno v: PLoS Pathogens, Vol 11, Iss 2, p e1004662 (2015)

Prions induce lethal neurodegeneration and consist of PrPSc, an aggregated conformer of the cellular prion protein PrPC. Antibody-derived ligands to the globular domain of PrPC (collectively termed GDL) are also neurotoxic. Here we show that GDL and

Externí odkaz: https://doaj.org/article/2f192076206246ed93ed98f65be2e327

Prion pathogenesis is faithfully reproduced in cerebellar organotypic slice cultures.

Autor: Jeppe Falsig, Tiziana Sonati, Uli S Herrmann, Dino Saban, Bei Li, Karina Arroyo, Boris Ballmer, Pawel P Liberski, Adriano Aguzzi

Publikováno v: PLoS Pathogens, Vol 8, Iss 11, p e1002985 (2012)

Prions cause neurodegeneration in vivo, yet prion-infected cultured cells do not show cytotoxicity. This has hampered mechanistic studies of prion-induced neurodegeneration. Here we report that prion-infected cultured organotypic cerebellar slices (C

Externí odkaz: https://doaj.org/article/4d93c9906020487fbbfde99e7148a661

Highly specific and selective anti-pS396-tau antibody C10.2 targets seeding-competent tau

Autor: Karsten Skjødt, Jan Egebjerg, Jan Torleif Pedersen, Fredrik Kartberg, Christiane Volbracht, Florence Sotty, Nina Helen Rosenqvist, Lars Østergaard Pedersen, Einar M. Sigurdsson, Ayodeji A. Asuni, Pia Jul, Christian Rungsted Andersson, Jeppe Falsig, Lone Helboe, Jeffrey B. Stavenhagen, Justus Claus Alfred Daechsel, Søren Christensen

Publikováno v: Alzheimer's & Dementia : Translational Research & Clinical Interventions
Rosenqvist, N, Asuni, A A, Andersson, C R, Christensen, S, Daechsel, J A, Egebjerg, J, Falsig, J, Helboe, L, Jul, P, Kartberg, F, Pedersen, L Ø, Sigurdsson, E M, Sotty, F, Skjødt, K, Stavenhagen, J B, Volbracht, C & Pedersen, J T 2018, ' Highly specific and selective anti-pS396-tau antibody C10.2 targets seeding-competent tau ', Alzheimer's & Dementia: Translational Research & Clinical Interventions, vol. 4, pp. 521-534 . https://doi.org/10.1016/j.trci.2018.09.005

Introduction: The abnormal hyperphosphorylation of the microtubule-associated protein tau plays a crucial role in neurodegeneration in Alzheimer's disease (AD) and other tauopathies.Methods: Highly specific and selective anti-pS396-tau antibodies hav

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a4e509d5c60e3458f1a368b67631b5a
https://pubmed.ncbi.nlm.nih.gov/30386817

Prion protein and Aβ-related synaptic toxicity impairment

Autor: Mario Nuvolone, Adriano Aguzzi, Osvaldo Mirante, Isabelle M. Mansuy, Rita Moos, Mélissa Farinelli, Jeppe Falsig, Anna Maria Calella

Publikováno v: EMBO Molecular Medicine

Alzheimer's disease (AD), the most common neurodegenerative disorder, goes along with extracellular amyloid-beta (Abeta) deposits. The cognitive decline observed during AD progression correlates with damaged spines, dendrites and synapses in hippocam

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a73c16f20e4e8d895603129b6940a9c3
http://europepmc.org/articles/PMC2962809

The prion organotypic slice culture assay—POSCA

Autor: Adriano Aguzzi, Jeppe Falsig

Publikováno v: Nature Protocols. 3:555-562

Methods enabling prion replication ex vivo are important for advancing prion science. However, few such technologies exist and many prion strains are intractable with them. Here, we describe a prion organotypic slice culture assay (POSCA), which allo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25a8afca9cb64de979bd3776944e5d02
https://doi.org/10.1038/nprot.2008.13