Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Jens Kastenhofer"'
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2021)
To date, high-pressure homogenization is the standard method for cell disintegration before the extraction of cytosolic and periplasmic protein from E. coli. Its main drawback, however, is low selectivity and a resulting high load of host cell impuri
Externí odkaz:
https://doaj.org/article/ca3fb7276a994272919d2ed685e3a040
Autor:
Jens Kastenhofer, Julian Ebner, Oliver Spadiut, Viktor L Sedlmayr, Alessandro Luigi Cataldo, Alois Jungbauer
Publikováno v:
Journal of Chemical Technology & Biotechnology. 96:1667-1674
BACKGROUND: Downstream processing of soluble recombinant proteins from Escherichia coli is complicated by the need to access the intracellular product by cell disruption and to separate the target protein from impurities, particularly host cell prote
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c074098114eace8b24dbf1bc9d3fe10a
https://doi.org/10.1002/jctb.6691
https://doi.org/10.1002/jctb.6691
Publikováno v:
Journal of biotechnology. 329
Soluble expression of recombinant proteins in E. coli is often done by translocation of the product across the inner membrane (IM) into the periplasm, where it is retained by the outer membrane (OM). While the integrity of the IM is strongly coupled
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b82b397d83a7cdb26d8b416c2f805dee
https://pubmed.ncbi.nlm.nih.gov/33485861
https://pubmed.ncbi.nlm.nih.gov/33485861
Publikováno v:
Frontiers in Bioengineering and Biotechnology
To date, high-pressure homogenization is the standard method for cell disintegration before the extraction of cytosolic and periplasmic protein from E. coli. Its main drawback, however, is low selectivity and a resulting high load of host cell impuri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::37fc1a2576ddfd471baf7da582d8a77f
https://pubmed.ncbi.nlm.nih.gov/33634078
https://pubmed.ncbi.nlm.nih.gov/33634078
Publikováno v:
Biotechnology journal. 16(3)
With the growing interest in continuous cultivation of Escherichia coli, secretion of product to the medium is not only a benefit, but a necessity in future bioprocessing. In this study, it is shown that induced decoupling of growth and heterologous
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18945cb920ce4f353db649ed399158a1
https://pubmed.ncbi.nlm.nih.gov/32915502
https://pubmed.ncbi.nlm.nih.gov/32915502
BackgroundRecombinant proteins in Escherichia coli are expressed inside the cell. With the growing interest in continuous cultivation, secretion of product to the medium is not only a benefit, but a necessity in future bioprocessing. In this study, w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::60ef04fd9dd202eb3391e414c31203ff
https://doi.org/10.21203/rs.3.rs-21294/v1
https://doi.org/10.21203/rs.3.rs-21294/v1
Autor:
Jens Kastenhofer, Oliver Spadiut
Publikováno v:
Journal of Biotechnology: X, Vol 6, Iss, Pp 100017-(2020)
During the expression of recombinant proteins in the periplasm of Escherichia coli, the integrity of the outer membrane can change, so that product leaks to the medium. Additional stress can induce lysis, the complete disintegration of both inner and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::95fe0b023e15c1c7fd91f327483aa09d
https://pubmed.ncbi.nlm.nih.gov/34154736
https://pubmed.ncbi.nlm.nih.gov/34154736
Autor:
Packo P. Lamers, Jacob A. de Hoop, Maria Barbosa, Jorijn H. Janssen, Jens Kastenhofer, René H. Wijffels
Publikováno v:
Algal Research 33 (2018)
Algal Research, 33, 125-132
Algal Research, 33, 125-132
Microalgae are a sustainable source of lipids. A commonly used strategy for lipid accumulation in microalgae is a two-step batch cultivation, with a growth phase followed by a nitrogen starvation phase. A problem with this process is the decrease in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::548916dc45f2eac6fc7f0a6180704a25
https://doi.org/10.1016/j.algal.2018.05.009
https://doi.org/10.1016/j.algal.2018.05.009
Publikováno v:
Processes
Volume 9
Issue 3
Processes, Vol 9, Iss 422, p 422 (2021)
Volume 9
Issue 3
Processes, Vol 9, Iss 422, p 422 (2021)
During recombinant protein production with E. coli, the integrity of the inner and outer membrane changes, which leads to product leakage (loss of outer membrane integrity) or lysis (loss of inner membrane integrity). Motivated by current Quality by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0211dcf41853c696d8357be6e740e95
https://doi.org/10.3390/pr9030422
https://doi.org/10.3390/pr9030422