Direct cell extraction of membrane proteins for structure–function analysis

Autor: Ieva Drulyte, Aspen Rene Gutgsell, Pilar Lloris-Garcerá, Michael Liss, Stefan Geschwindner, Mazdak Radjainia, Jens Frauenfeld, Robin Löving

Publikováno v: Scientific Reports, Vol 13, Iss 1, Pp 1-8 (2023)

Abstract Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins d

Externí odkaz: https://doaj.org/article/f178d0ff85c94367be68f1fb3cadfd98

SecM-stalled ribosomes adopt an altered geometry at the peptidyl transferase center.

Autor: Shashi Bhushan, Thomas Hoffmann, Birgit Seidelt, Jens Frauenfeld, Thorsten Mielke, Otto Berninghausen, Daniel N Wilson, Roland Beckmann

Publikováno v: PLoS Biology, Vol 9, Iss 1, p e1000581 (2011)

As nascent polypeptide chains are synthesized, they pass through a tunnel in the large ribosomal subunit. Interaction between specific nascent chains and the ribosomal tunnel is used to induce translational stalling for the regulation of gene express

Externí odkaz: https://doaj.org/article/7da6875e5218435da7769ad4cdeb4228

DirectMX – One-Step Reconstitution of Membrane Proteins From Crude Cell Membranes Into Salipro Nanoparticles

Autor: Stefan Klinter, Michael J. Skynner, Pilar Lloris-Garcerá, Jens Frauenfeld, Liuhong Chen, Robin Löving

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Frontiers in Bioengineering and Biotechnology

Integral membrane proteins (IMPs) are central to many physiological processes and represent ∼60% of current drug targets. An intricate interplay with the lipid molecules in the cell membrane is known to influence the stability, structure and functi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::898865f53824758034f1fe65ce434222
https://doi.org/10.3389/fbioe.2020.00215

Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters

Autor: Jens Frauenfeld, Joseph A. Lyons, Poul Nissen, Andreas Bøggild

Publikováno v: Lyons, J A, Bøggild, A, Nissen, P & Frauenfeld, J 2017, Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters . in C Ziegler (ed.), Methods in Enzymology : A Structure-Function Toolbox for Membrane Transporter and Channels . vol. 594, Academic Press, Methods in Enzymology, vol. 594, pp. 85-99 . https://doi.org/10.1016/bs.mie.2017.06.035
Methods in Enzymology
Methods in Enzymology-A Structure-Function Toolbox for Membrane Transporter and Channels

Membrane proteins depend on their natural lipid environment for function, which makes them more difficult to study in isolation. A number of approaches that mimic the lipid bilayer of biological membranes have been described (nanodiscs, SMALPs), enab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7463b77c7025a272bb5c410b937e3ec3
https://pure.au.dk/portal/da/publications/saposinlipoprotein-scaffolds-for-structure-determination-of-membrane-transporters(198c168b-5c8c-4ce4-9a0f-407924a6f39a).html

High-throughput analytical gel filtration screening of integral membrane proteins for structural studies

Autor: Jens Frauenfeld, Fatma Guettou, Esben M. Quistgaard, Pär Nordlund, Lars Haneskog, Per Moberg, Marie Hedrén, Christian Löw

Publikováno v: Biochimica et biophysica acta. 1830(6)

Background Structural studies of integral membrane proteins (IMPs) are often hampered by difficulties in producing stable homogenous samples for crystallization. To overcome this hurdle it has become common practice to screen large numbers of target

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8595a029215093ae9d4d0d5d8b7d6f55
https://pubmed.ncbi.nlm.nih.gov/23403133