Short locked nucleic acid antisense oligonucleotides potently reduce apolipoprotein B mRNA and serum cholesterol in mice and non-human primates

Autor: Niels Fisker, Ellen Marie Straarup, Henrik Ørum, Henrik Frydenlund Hansen, Christoph Rosenbohm, Jens Bo Hansen, Vibeke Aarup, Troels Koch, Marie Lindholm, Maj Hedtjärn

Publikováno v: Nucleic Acids Research

The potency and specificity of locked nucleic acid (LNA) antisense oligonucleotides was investigated as a function of length and affinity. The oligonucleotides were designed to target apolipoprotein B (apoB) and were investigated both in vitro and in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87022396d3372bd00e7c4e8fde7e622d
https://doi.org/10.1093/nar/gkq457

Synthesis and Biological Evaluation of LNA Phosphoramidates

Autor: Jacob Jensen, Gitte Sjøgren, Christoph Rosenbohm, Jens Bo Hansen, Troels Koch

Publikováno v: Nucleosides, Nucleotides and Nucleic Acids. 27:37-42

The synthesis of LNA phosphoramidates is presented. The LNA phosphoramidates were evaluated for their ability to inhibit cell proliferation of the human prostate cancer cell line 15PC3. A number of the LNA phosphoramidates showed cell proliferation i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a0b7a54dff4b50eed69e8ae80fd1a5a
https://doi.org/10.1080/15257770701571834

Aptamers Targeted to an RNA Hairpin Show Improved Specificity Compared to that of Complementary Oligonucleotides

Autor: Sandrine Reigadas, Jens Bo Hansen, Carmelo Di Primo, Jean-Jacques Toulmé, Henrik Ørum, Fabien Darfeuille

Publikováno v: Biochemistry. 45:12076-12082

Aptamers interacting with RNA hairpins through loop-loop (so-called kissing) interactions have been described as an alternative to antisense oligomers for the recognition of RNA hairpins. R06, an RNA aptamer, was previously shown to form a kissing co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e65b7c8d831b801463b22e3e7bde317
https://doi.org/10.1021/bi0606344

Locked nucleic acid antisense oligonucleotides targeting apolipoprotein B: the effect of short sequences and α-L-LNA insertion

Autor: Niels Fisker, Ellen Marie Straarup, Michael Meldgaard, Jens Bo Hansen, Christoph Rosenbohm, Joachim Elmén, Maj Hedtjärn, Marie W. Lindblom, Henrik Hansen, Jacob Ravn

Publikováno v: Collection Symposium Series.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2acb971c7d0e14c5ef0f1eaba8edea3e
https://doi.org/10.1135/css201112444

Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver

Autor: Keith Mccullagh, Mette Christensen, Jens Bo Hansen, Sakari Kauppinen, Ellen Marie Straarup, Phil Kearney, Maj Hedtjärn, Allan Lind-Thomsen, Joacim Elmén, Mads Bak, Henrik Hansen, Asli Silahtaroglu, Morten Lindow

Publikováno v: Elmen, J, Lindow, M, Silahtaroglu, A, Bak, M, Christensen, M, Lind-Thomsen, A, Hedtjarn, M, hansen, J B, Hansen, H F, Straarup, E M, McCullagh, K, Keraney, P & Kauppinen, M S 2008, ' Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver ', Nucleic Acids Research, vol. 36, no. 4, pp. 1153-1162 . https://doi.org/PMID: 1815830410.1093/nar/gkm1113
Nucleic Acids Research

Udgivelsesdato: 2008 Mar MicroRNA-122 (miR-122) is an abundant liver-specific miRNA, implicated in fatty acid and cholesterol metabolism as well as hepatitis C viral replication. Here, we report that a systemically administered 16-nt, unconjugated LN

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::737501d926db11e92f89c35ab23bf06a
https://curis.ku.dk/ws/files/10189260/1153.pdf