Výsledky vyhledávání - "Jenny A. Lounsbury"

Isolation of a Low Number of Sperm Cells from Female DNA in a Glass-PDMS-Glass Microchip via Bead-Assisted Acoustic Differential Extraction

Autor: Johan Nilsson, Charles P. Clark, Thomas Laurell, Kerui Xu, Brian L. Poe, James P. Landers, Jenny A. Lounsbury

Publikováno v: Analytical chemistry. 91(3)

We report an improved separation method for the isolation of sperm cells from dilute, "large volume" samples containing female DNA using bead-assisted acoustic trapping. In an enclosed glass-PDMS-glass (GPG) resonator, we exploit a three-layer microf

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb29774510a6283614e799c6093bdb6e
https://pubmed.ncbi.nlm.nih.gov/30652864

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Enhanced recovery of spermatozoa and comprehensive lysis of epithelial cells from sexual assault samples having a low cell counts or aged up to one year

Autor: Helina Cunniffe, Shanti M. Nambiar, Jessica V. Norris, Jerome P. Ferrance, Anne Karlsson, Jenny A. Lounsbury, James P. Landers

Publikováno v: Forensic Science International: Genetics. 8:84-89

Differential extraction (DE) is the most common method for processing sexual assault samples, allowing for the simultaneous recovery of sperm and epithelial cells from the swab with the separation of sperm cells from epithelial cell DNA by exploiting

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b67262246ce1d4aad0246a4ac6392af
https://doi.org/10.1016/j.fsigen.2013.06.015

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Ultrafast Amplification of DNA on Plastic Microdevices for Forensic Short Tandem Repeat Analysis

Autor: Jenny A. Lounsbury, James P. Landers

Publikováno v: Journal of Forensic Sciences. 58:866-874

The majority of microfluidic devices used as a platform for low-cost, rapid DNA analysis are glass devices; however, microchip fabrication in glass is costly and laborious, enhancing the interest in polymeric substrates, such as poly (methyl methacry

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d9a31264bc293c3bd63ef74c27e2ca7
https://doi.org/10.1111/1556-4029.12162

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Sample-to-Result STR Genotyping Systems: Potential and Status

Autor: Joan M. Bienvenue, Jenny A. Lounsbury, James P. Landers

Publikováno v: Forensic DNA Analysis ISBN: 9780429254338

Forensic DNA analysis using short tandem repeats (STRs) has become the cornerstone for human identification, kinship analysis, paternity testing, and other applications. However, it is a lengthy, laborious process that requires specialized training a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ce6257b7fe762345ef7b5d4bead56893
https://doi.org/10.1201/b15361-19

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From sample to PCR product in under 45 minutes: a polymeric integrated microdevice for clinical and forensic DNA analysis

Autor: Paul Kinnon, Stephen M. Cronk, Doris M. Haverstick, James P. Landers, David J. Saul, Anne Karlsson, Jenny A. Lounsbury, Daniel C. Miranian, Jingyi Li, Daniel Nelson

Publikováno v: Lab on a chip. 13(7)

The extraction and amplification of DNA from biological samples is laborious and time-consuming, requiring numerous instruments and sample handling steps. An integrated, single-use, poly(methyl methacrylate) (PMMA) microdevice for DNA extraction and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bcdc342678048fa4204d7f390d6efa8
https://pubmed.ncbi.nlm.nih.gov/23389252

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Label-free DNA quantification via a 'pipette, aggregate and blot' (PAB) approach with magnetic silica particles on filter paper

Autor: Doris M. Haversitick, Jenny A. Lounsbury, Jingyi Li, Hussein Alsamarri, Qian Liu, James P. Landers

Publikováno v: Lab on a chip. 13(5)

Reliable measurement of DNA concentration is essential for a broad range of applications in biology and molecular biology, and for many of these, quantifying the nucleic acid content is inextricably linked to obtaining optimal results. In its most si

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::968288c92097b7500955bf0511c082c9
https://pubmed.ncbi.nlm.nih.gov/23338039

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An enzyme-based DNA preparation method for application to forensic biological samples and degraded stains

Autor: Natalie Coult, Doris M. Haverstick, David J. Saul, Daniel C. Miranian, Stephen M. Cronk, Paul Kinnon, James P. Landers, Jenny A. Lounsbury

Publikováno v: Forensic science international. Genetics. 6(5)

Extraction of DNA from forensic samples typically uses either an organic extraction protocol or solid phase extraction (SPE) and these methods generally involve numerous sample transfer, wash and centrifugation steps. Although SPE has been successful

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78af058efda87ea5fe5be5936470d410
https://pubmed.ncbi.nlm.nih.gov/22459950

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Laser-ablated poly(methyl methacrylate) microdevices for sub-microliter DNA amplification suitable for micro-total analysis systems

Autor: Michael Do, Jenny A. Lounsbury, Brian L. Poe, James P. Landers

Publikováno v: Journal of Micromechanics and Microengineering. 22:085006

Biochemical techniques, such as the polymerase chain reaction (PCR), can take up to 3.5?h for completion using a commercial bench-top instrument, creating a bottleneck in sample preparation processes. PCR has been successfully adapted to microfluidic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::36c42115d50ea46441f2a87bcd8b806f
https://doi.org/10.1088/0960-1317/22/8/085006

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