Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Jennifer N. Hennigan"'
Publikováno v:
BioTechniques, Vol 71, Iss 5, Pp 566-572 (2021)
Autoinducible, two-stage protein expression leveraging phosphate-inducible promoters has been recently shown to enable not only high protein titers but also consistent performance across scales from screening systems (microtiter plates) to instrument
Externí odkaz:
https://doaj.org/article/8849a228d09c4174b6c003cd9dbe450a
Publikováno v:
BioTechniques. 71:566-572
Autoinducible, two-stage protein expression leveraging phosphate-inducible promoters has been recently shown to enable not only high protein titers but also consistent performance across scales from screening systems (microtiter plates) to instrument
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b2b6a37de9ab6fe3c886785e0b508b09
https://doi.org/10.2144/btn-2021-0055
https://doi.org/10.2144/btn-2021-0055
Publikováno v:
J Control Release
The use of biologics (peptide and protein based drugs) has increased significantly over the past few decades. However, their development has been limited by their short half-life, immunogenicity and low membrane permeability, restricting most therapi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46f9738551d70c81a420d5738e8f7e01
https://doi.org/10.1016/j.jconrel.2018.12.032
https://doi.org/10.1016/j.jconrel.2018.12.032
Autoinducible, 2-stage protein expression leveraging phosphate inducible promoters has been recently shown to enable not only high protein titers but also consistent performance across scales from screening systems (microtiter plates) to instrumented
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3172f3fc547301fc6c32d79b785faac1
https://doi.org/10.1101/2021.06.08.447502
https://doi.org/10.1101/2021.06.08.447502
Autor:
Burk C, Wagner P, Jennifer N. Hennigan, Ye Z, John P. Efromson, Michael D. Lynch, M.L. Lipscomb
Fermentation based bioprocesses are routinely developed for higher value products; however, the development of bioprocesses for larger volume chemicals has lagged. While electrification of transportation may enable significant reductions in greenhous
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::02851e8343a88b5d24a5193926c4ed75
https://doi.org/10.26434/chemrxiv.13238996.v1
https://doi.org/10.26434/chemrxiv.13238996.v1
Autor:
Shuai Li, Tian Yang, Jennifer N. Hennigan, Eirik A. Moreb, Michael D. Lynch, Zhixia Ye, Daniel Baez Castellanos
Publikováno v:
Metabolic engineering. 64
We report improved NADPH flux and xylitol biosynthesis in engineered E. coli. Xylitol is produced from xylose via an NADPH dependent reductase. We utilize 2-stage dynamic metabolic control to compare two approaches to optimize xylitol biosynthesis, a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a513035a6548db2fd68b7ff06cd84de5
https://pubmed.ncbi.nlm.nih.gov/33460820
https://pubmed.ncbi.nlm.nih.gov/33460820
Autor:
Shuzi Li, Jennifer N. Hennigan, Eirik A. Moreb, Daniel Baez Castellanos, Tian Yang, Michael D. Lynch, Zhixia Ye
We report improved NADPH flux and xylitol biosynthesis in engineered E. coli. Xylitol is produced from xylose via an NADPH dependent reductase. We utilize two-stage dynamic metabolic control to compare two approaches to optimize xylitol biosynthesis,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::df08fcf92e97b0d543b8d5075a144749
https://doi.org/10.1101/2020.07.27.222588
https://doi.org/10.1101/2020.07.27.222588
Autor:
Eirik A. Moreb, Michael D. Lynch, Jennifer N. Hennigan, Romel Menacho-Melgar, John P. Efromson, Zhixia Ye
Publikováno v:
ACS synthetic biology. 9(6)
A key challenge in synthetic biology is the successful utilization of characterized parts, such as promoters, in different biological contexts. We report the evaluation of the media robustness of a small library of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6fa488b41ffee0c667d074df756c200
https://pubmed.ncbi.nlm.nih.gov/32353228
https://pubmed.ncbi.nlm.nih.gov/32353228
Autor:
Eirik A. Moreb, Romel Menacho-Melgar, Michael D. Lynch, John P. Efromson, Zhixia Ye, Jennifer N. Hennigan
A key challenge in synthetic biology is the successful utilization of characterized parts, such as promoters, in different biological contexts. We report the robustness testing of a small library of E. coli PhoB regulated promoters that enable hetero
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6889ce03d9f6daec47ce0fec6f5d6ca6
https://doi.org/10.1101/2020.01.26.920280
https://doi.org/10.1101/2020.01.26.920280
Autor:
Jennifer N. Hennigan, Michael D. Lynch, Tian Yang, Romel Menacho-Melgar, Eirik A. Moreb, John P. Efromson, Ruixin Wang
Publikováno v:
Biotechnology and bioengineeringREFERENCES. 117(9)
We report improved release of recombinant proteins in Escherichia coli, which relies on combined cellular autolysis and DNA/RNA autohydrolysis, conferred by the tightly controlled autoinduction of both phage lysozyme and the nonspecific DNA/RNA endon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84ef73dab8f82a220b196bcbfc8bf06b
https://pubmed.ncbi.nlm.nih.gov/32462658
https://pubmed.ncbi.nlm.nih.gov/32462658