Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Jennifer M. Zupancic"'
Autor:
Jennifer M. Zupancic, Matthew D. Smith, Hanna Trzeciakiewicz, Mary E. Skinner, Sean P. Ferris, Emily K. Makowski, Michael J. Lucas, Nikki McArthur, Ravi S. Kane, Henry L. Paulson, Peter M. Tessier
Publikováno v:
Frontiers in Immunology, Vol 14 (2023)
Single-domain antibodies, also known as nanobodies, are broadly important for studying the structure and conformational states of several classes of proteins, including membrane proteins, enzymes, and amyloidogenic proteins. Conformational nanobodies
Externí odkaz:
https://doaj.org/article/744b44e777364c28a6fe9024584e8b23
Autor:
Emily K. Makowski, Patrick C. Kinnunen, Jie Huang, Lina Wu, Matthew D. Smith, Tiexin Wang, Alec A. Desai, Craig N. Streu, Yulei Zhang, Jennifer M. Zupancic, John S. Schardt, Jennifer J. Linderman, Peter M. Tessier
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Optimising antibody properties such as affinity can be detrimental to other key properties. Here the authors use machine learning to simplify the identification of antibodies with co-optimal levels of affinity and specificity for a clinical-stage ant
Externí odkaz:
https://doaj.org/article/0014043dced04499aebddc31d0764ef0
Autor:
John S. Schardt, Ghasidit Pornnoppadol, Alec A. Desai, Kyung Soo Park, Jennifer M. Zupancic, Emily K. Makowski, Matthew D. Smith, Hongwei Chen, Mayara Garcia de Mattos Barbosa, Marilia Cascalho, Thomas M. Lanigan, James J. Moon, Peter M. Tessier
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Abstract Monoclonal antibodies that target SARS-CoV-2 with high affinity are valuable for a wide range of biomedical applications involving novel coronavirus disease (COVID-19) diagnosis, treatment, and prophylactic intervention. Strategies for the r
Externí odkaz:
https://doaj.org/article/75354dc4a1d54b489cf8d0e0fd26f7ef
Autor:
Emily K. Makowski, Hongwei Chen, Matthew Lambert, Eric M. Bennett, Nicole S. Eschmann, Yulei Zhang, Jennifer M. Zupancic, Alec A. Desai, Matthew D. Smith, Wenjia Lou, Amendra Fernando, Timothy Tully, Christopher J. Gallo, Laura Lin, Peter M. Tessier
Publikováno v:
mAbs, Vol 14, Iss 1 (2022)
Self-association governs the viscosity and solubility of therapeutic antibodies in high-concentration formulations used for subcutaneous delivery, yet it is difficult to reliably identify candidates with low self-association during antibody discovery
Externí odkaz:
https://doaj.org/article/b7a6b619288444029f50f9d427dbd60c
Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis
Publikováno v:
STAR Protocols, Vol 3, Iss 1, Pp 101101- (2022)
Summary: The generation of high-affinity nanobodies for diverse biomedical applications typically requires immunization or affinity maturation. Here, we report a simple protocol using complementarity-determining region (CDR)-swapping mutagenesis to i
Externí odkaz:
https://doaj.org/article/35b4cd6716784b719aaf0326f1a20c56
Autor:
Ghasidit Pornnoppadol, Layne G. Bond, Michael J. Lucas, Jennifer M. Zupancic, Yun-Huai Kuo, Boya Zhang, Colin F. Greineder, Peter M. Tessier
Publikováno v:
bioRxiv
SUMMARYThe inability of antibodies and other biologics to penetrate the blood-brain barrier (BBB) is a key limitation to their use in diagnostic, imaging, and therapeutic applications. One promising strategy is to deliver IgGs using a bispecific BBB
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9cb0ba3d46b29b735f7c5131a8b1d26
https://europepmc.org/articles/PMC10168297/
https://europepmc.org/articles/PMC10168297/
Publikováno v:
Methods in Molecular Biology ISBN: 9781071622841
Methods Mol Biol
Methods Mol Biol
Conformational antibodies specific for amyloid-forming peptides and proteins are important for a range of biomedical applications, including detecting, inhibiting, and potentially treating protein aggregation disorders ranging from Alzheimer's to Par
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63dee8dc620e4a18f26c56bb7f2d799a
https://doi.org/10.1007/978-1-0716-2285-8_22
https://doi.org/10.1007/978-1-0716-2285-8_22
Autor:
Jennifer M. Zupancic, John S. Schardt, Alec A. Desai, Emily K. Makowski, Matthew D. Smith, Ghasidit Pornnoppadol, Mayara Garcia de Mattos Barbosa, Marilia Cascalho, Thomas M. Lanigan, Peter M. Tessier
Publikováno v:
Advanced Therapeutics
The COVID‐19 pandemic continues to be a severe threat to human health, especially due to current and emerging SARS‐CoV‐2 variants with potential to escape humoral immunity developed after vaccination or infection. The development of broadly neu
Autor:
Jennifer M. Zupancic, Shannon J. Moore, Henry L. Paulson, Alexandra B. Sutter, Edward Ionescu, Julia E. Gerson, Magdalena I. Ivanova, Yulei Zhang, Geoffrey G. Murphy, Peter M. Tessier, Matthew D. Smith, Alec A. Desai, Charles G. Starr, Emily K. Makowski
Publikováno v:
The Journal of Biological Chemistry
The aggregation of amyloidogenic polypeptides is strongly linked to several neurodegenerative disorders, including Alzheimer's and Parkinson's diseases. Conformational antibodies that selectively recognize protein aggregates are leading therapeutic a
Autor:
Andrew W. Tai, Andrew A Kennedy, Mayara Garcia de Mattos Barbosa, John S. Schardt, Alec A. Desai, Emily K. Makowski, Ghasidit Pornnoppadol, Peter M. Tessier, Marilia Cascalho, Jennifer M. Zupancic, Matthew D. Smith, Thomas M. Lanigan
Publikováno v:
Cell Chemical Biology
There is widespread interest in facile methods for generating potent neutralizing antibodies, nanobodies, and other affinity proteins against SARS-CoV-2 and related viruses to address current and future pandemics. While isolating antibodies from anim