Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Jen I. Mao"'
Autor:
Jen-I Mao, Donald T. Moir
Secretion is an attractive production mode for proteins that require posttranslational modifications carried out in the secretory pathway. For example, amino acid chains fold properly, disulfide bonds form correctly, and glycosylation occurs accurate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::37f65dcb3994af5e49443720a7e30135
https://doi.org/10.1201/9781003066392-5
https://doi.org/10.1201/9781003066392-5
Autor:
David H. Lloyd, Kevin Corcoran, George Stefan Golda, Michael F. Foy, James W. Kirchner, Rithy Roth, Sydney Brenner, Sarah N. McCurdy, Dave George, Robert B. Dubridge, Sam Eletr, Mark Ewan, Steven Williams, Maria Johnson, Eric Vermaas, John Bridgham, Michael C. Pallas, Timothy Burcham, Karen L. Fearon, Keith Moon, Davida Johnson, Glenn Albrecht, Shujun Luo, Jen-I Mao
Publikováno v:
Nature Biotechnology. 18:630-634
We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro clon
Autor:
Simone Schuffenhauer, Lynne Hobson, Grant R. Sutherland, Jen-i Mao, Elizabeth Baker, Robert I. Richards, Marie Mangelsdorf, Oliva Handt, Helen J. Eyre, Duncan R. Hewett
Publikováno v:
Molecular Cell. 1:773-781
A common mechanism for chromosomal fragile site genesis is not yet apparent. Folate-sensitive fragile sites are expanded p(CCG)n repeats that arise from longer normal alleles. Distamycin A or bromodeoxyuridine-inducible fragile site FRA16B is an expa
Autor:
Deepika Madan, James H. Smith, Marcy Engelstein, Jen-i Mao, Tyler J. Aldredge, Douglas R. Smith, Phil W. Rice
Publikováno v:
Microbial & Comparative Genomics. 3:237-241
We have developed a 96-well format for DNA template isolation that can be readily automatable. The template isolation protocol involves simple alkaline lysis chemistry and reversible capture on a silica solid phase. After the cells are lysed, no cent
Publikováno v:
American Journal of Medical Genetics. 61:16-20
We describe a family in which two siblings exhibited developmental delay, reduced muscle tone and mild muscle weakness. Cytogenetic evaluation demonstrated that both children had a tandem duplication of a small portion of the long arm of chromosome 1
Autor:
Mitchell S. Rein, Jonathan A. Fletcher, Sung-Joo Yoon, Cynthia C. Morton, Jen-I Mao, Marlena S. Fejzo, Donald T. Moir, Raju Kucherlapati, Kate T. Montgomery, Kenneth S. Krauter, Stanislawa Weremowicz, Thomas E. Dorman
Publikováno v:
Genomics. 26:265-271
Uterine leiomyomata are the most common tumors in women and can cause abnormal uterine bleeding, pelvic pain, and infertility. Approximately 200,000 hysterectomies are performed annually in the U.S. to relieve patients of the medical sequelae of thes
Publikováno v:
Genetic Epidemiology. 12:303-308
The human dopamine transporter gene, DAT1, acts to transport released dopamine into presynaptic terminals of the brain. The possibility that the DAT1 gene plays a role in genetic diseases of the brain has led to studies of DAT1 in several psychiatric
Publikováno v:
Genomics. 22:1-12
One hundred eighty-three YACs carrying human chromosome 10 sequences were isolated from multigenome equivalent libraries by PCR-based screening for the presence of 80 different chromosome 10-specific STSs. Ninety-four of the isolated YACs, representi
Autor:
Tim Keith, Karen Braunschweiger, Mei-Tai Wang, Nancy Shui-Fong Ma, Jen-I Mao, Melissa K. Schuster, Donald T. Moir, Donald W. Bowden, Cynthia B. Rothschild, Dana Torrey, Thomas E. Dorman, Chuang-jie Zheng, Lorena Soares
Publikováno v:
Genomics. 22:55-67
A total of 124 new chromosome 10-specific sequenced-tagged sites (STSs) were derived from two sources: (1) DNA sequences obtained from anonymous clones in new libraries enriched for human chromosome 10 inserts, and (2) published sequences of genes an
Autor:
Kathy Hsiao, Jen-i Mao, Denis Drapeau, Jeff S. Socolow, George C. Avgerinos, Robert J. Broeze
Publikováno v:
Nature Biotechnology. 8:54-58
We have used a 20 liter stirred tank fermentor, equipped with a 127 mesh ethylene-tetrafluoroethylene rotating screen for cell recycle, for the continuous production of recombinant single chain urokinase-type plasminogen activator (rscu-PA) from Chin