Biochemical characterization of mutants in the active site residues of the β-galactosidase enzyme of Bacillus circulans ATCC 31382

Autor: Jelle B. Bultema, Bas J.H. Kuipers, Lubbert Dijkhuizen

Publikováno v: FEBS Open Bio, Vol 4, Iss C, Pp 1015-1020 (2014)

The Bacillus circulans ATCC 31382 β-galactosidase (BgaD) is a retaining-type glycosidase of glycoside hydrolase family 2 (GH2). Its commercial enzyme preparation, Biolacta N5, is used for commercial-scale production of galacto-oligosaccharides (GOS)

Externí odkaz: https://doaj.org/article/88f68fd6b94d4e2a9890a76f0e48f621

Light-Responsive Capture and Release of DNA in a Ternary Supramolecular Complex

Autor: Siva Krishna Mohan Nalluri, Bart Jan Ravoo, Jelle B. Bultema, Jens Voskuhl, Egbert J. Boekema

Publikováno v: Angewandte Chemie-International Edition, 50(41), 9747-9751. WILEY-V C H VERLAG GMBH

The wavelength determines whether DNA is captured in a light-responsive ternary supramolecular complex or released (see scheme). The reversible binding of DNA is triggered by a photoisomerization, which switches the complex from a multivalent to a mo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb0e92480846df6130a56745c93e0819
https://doi.org/10.1002/anie.201103707

Structural basis for CRISPR RNA-guided DNA recognition by Cascade

Autor: Marieke R Beijer, Mark J. Dickman, Edze R. Westra, Kaihong Zhou, Sakharam P Waghmare, Rolf Wagner, Reinhild Wurm, Jennifer A. Doudna, Egbert J. Boekema, Stan J. J. Brouns, Magnus Lundgren, Blake Wiedenheft, Ümit Pul, Matthijs M. Jore, Esther van Duijn, Albert J. R. Heck, Arjan Barendregt, Ambrosius P. Snijders, Jelle B. Bultema, John van der Oost

Publikováno v: Nature Structural and Molecular Biology 18 (2011) 5
Nature Structural and Molecular Biology, 18(5), 529-536
Nature Structural & Molecular Biology, 18(5), 529-U141. Nature Publishing Group

The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids. In Escherichia coli, immunity depends on a ribonucleoprotein complex called Cascade

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8abbd22b45b02680b6fa2a57df925d77
https://research.rug.nl/en/publications/5d890c0e-4998-47cd-a8b4-4b4c4c0b0178

Imaging of organelles by electron microscopy reveals protein-protein interactions in mitochondria and chloroplasts

Autor: Egbert J. Boekema, Jelle B. Bultema, Roman Kouřil, Natalya V. Dudkina

Publikováno v: FEBS Letters. 584:2510-2515

Ongoing progress in electron microscopy (EM) offers now an opening to visualize cells at the nanoscale by cryo-electron tomography (ET). Large protein complexes can be resolved at near-atomic resolution by single particle averaging. Some examples fro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ca4e4c969a29fe8b70792b77cb57f2e
https://doi.org/10.1016/j.febslet.2010.03.027

Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-amylase-like transglycosylase

Autor: Jelle B. Bultema, Thomas R. M. Barends, Marc J. E. C. van der Maarel, Lubbert Dijkhuizen, Thijs Kaper, Bauke W. Dijkstra

Publikováno v: The Journal of Biological Chemistry, 282(23), 17242-17249. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Amylomaltases are glycosyl hydrolases belonging to glycoside hydrolase family 77 that are capable of the synthesis of large cyclic glucans and the disproportionation of oligosaccharides. Using protein crystallography, we have generated a flip book mo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6be9ccc0f4c85d6d5d75c4f9d7f76cd
https://hdl.handle.net/11370/0f40cb4c-291b-41fd-ac72-cdd25f4cc858