Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Jekisiel Szulmajster"'
Publikováno v:
European journal of biochemistry. 194(1)
The two genes of Bacillus sphaericus 1953M coding for the 51.4-kDa and 41.9-kDa proteins are both required for the expression of the active larvicidal toxin in Escherichia coli. The minimal size of the active peptide of the 41.9-kDa toxin was defined
Autor:
Jekisiel Szulmajster, Pierre Kerjan
Publikováno v:
Biochimie. 58:533-541
Summary A ribonuclease was isolated and completely purified from sporulating cells of Bacillus subtilis . This RNase has a M.W. of about 150,000 daltons. It hydrolyzes single stranded RNA and single stranded synthetic polynucleotides yielding nucleos
Autor:
Jekisiel Szulmajster, Céline Bonamy
Publikováno v:
Molecular and General Genetics MGG. 188:202-210
Two spore genes, spoOB and spoIIG have been cloned from the B. subtilis genome library, constructed by ligating Sau3A partially digested DNA to the dephosphorylated pHV33 plasmid vector at its BamH1 site.
Autor:
Jekisiel Szulmajster
Publikováno v:
Trends in Biochemical Sciences. 4:18-22
Bacterial sporulation is generally considered to be a simple model of cellular differentiation. However, the extensive genetic and biochemical studies of the last few years have revealed an unexpected complexity in this system.
Publikováno v:
Biochemical and Biophysical Research Communications. 143:901-907
We present here a procedure for purifying the larvicidal toxin from sporulating cells of Bacillus sphaericus 1593M and describe some of the biochemical and biophysical properties of this toxin. The procedure involves solubilization of the cell-wall/m
Publikováno v:
FEMS Microbiology Letters. 10:273-276
Publikováno v:
Proceedings of the National Academy of Sciences. 82:4189-4192
Evidence is presented that the sporulation-essential locus spoIIG codes for both sigma 29 and a structurally related protein, P31. This demonstrates that at least one specific Bacillus subtilis RNA polymerase binding protein provides a critical funct
Publikováno v:
Molecular and General Genetics MGG. 185:448-453
cDNA probes synthesized on poly(A)RNAs isolated from sporulating cells of Bacillus subtilis were used for hybridization studies with RNAs derived from cells at different stages of growth and sporulation. It was shown that these cDNAs hybridized only
Publikováno v:
European Journal of Biochemistry. 98:353-362
A thermosensitive sporulation mutant (ts-15) of Bacillus subtilis has been isolated. This mutant when grown at the restrictive temperature (42 degrees C) is unable to sporulate, shows no intracellular protease activity and no protein turnover. These
Autor:
Jekisiel Szulmajster
Publikováno v:
Biochimica et Biophysica Acta. 30:154-163
The degradation of creatinine to NH3 and N-methylhydantoin by crude cell-free extracts of Cl. paraputrificum is coupled with the formation of ATP and orthophosphate. However, an enzyme which has been purified mre than 4000 fold is able to carry out a