Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Jehan Lee"'
Publikováno v:
BioTechniques, Vol 36, Iss 3, Pp 398-400 (2004)
Externí odkaz:
https://doaj.org/article/71800b8ee4de4a0297ca6ea972a558c2
Publikováno v:
Journal of Virology. 78:6252-6262
Hepadnaviruses replicate through reverse transcription of an RNA pregenome, resulting in a relaxed circular DNA genome. The first 3 or 4 nucleotides (nt) of minus-strand DNA are synthesized by the use of a bulge in a stem-loop structure near the 5′
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::541ad8d66efe09068af4e83bc49a341b
https://doi.org/10.1128/jvi.78.12.6252-6262.2004
https://doi.org/10.1128/jvi.78.12.6252-6262.2004
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 634
Mutagenesis by the overlap extension PCR has become a standard method of creating mutations including substitutions, insertions, and deletions. Nonetheless, the established overlap PCR mutagenesis is limited in many respects. In particular, it has be
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::9579ba01c58d80d0b8c8cedb7440d9bf
https://pubmed.ncbi.nlm.nih.gov/20676981
https://pubmed.ncbi.nlm.nih.gov/20676981
Publikováno v:
Methods in Molecular Biology ISBN: 9781607616511
Mutagenesis by the overlap extension PCR has become a standard method of creating mutations including substitutions, insertions, and deletions. Nonetheless, the established overlap PCR mutagenesis is limited in many respects. In particular, it has be
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b57726e4b889b98a7467519b7315f85e
https://doi.org/10.1007/978-1-60761-652-8_10
https://doi.org/10.1007/978-1-60761-652-8_10
Publikováno v:
Molecules and cells. 25(4)
A hepadnaviruses replicates its DNA genome via reverse transcription of an RNA template (pregenomic RNA or pgRNA), which has a cap structure at the 5' end and a poly(A) tail at the 3' end. We have previously shown that the 5' cap is indispensable for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d0765b3d31f4a66a60da4273820351ba
https://pubmed.ncbi.nlm.nih.gov/18460902
https://pubmed.ncbi.nlm.nih.gov/18460902
Synthesis of the relaxed-circular (RC) DNA genomes of hepadnaviruses by reverse transcriptase involves two template switches during plus-strand DNA synthesis. These template switches require repeat sequences (so-called donor and acceptor sites) betwe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::481bc366a9548b5a51bf7c548b16c659
https://europepmc.org/articles/PMC434075/
https://europepmc.org/articles/PMC434075/
Publikováno v:
Scopus-Elsevier
based procedure that can be used to generate precisely located mutants with any length of insertion or deletion. The strategy for insertion mutagenesis employs an established overlap extension PCR protocol, but involves three templates including an i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc22ede8405e2f35d6c2377bb19405fe
https://pubmed.ncbi.nlm.nih.gov/15038153
https://pubmed.ncbi.nlm.nih.gov/15038153
Publikováno v:
Journal of Virology. Aug2009, Vol. 83 Issue 16, p8032-8040. 9p.