Zobrazeno 1 - 10
of 57
pro vyhledávání: '"Jeffrey R. Fishpaugh"'
Autor:
Khandaker Siddiquee, Cheng Zhao, Michelle A. Stemler, Bill Zeck, Jeffrey R. Fishpaugh, Steven P. Allen
Publikováno v:
Bioresources and Bioprocessing, Vol 6, Iss 1, Pp 1-15 (2019)
Abstract Use of Quality-by-Design (QbD) tools is becoming an important part of the bioprocessing industry when developing a process for manufacturing operations to ensure the robustness and reproducibility of the biologic product. In the present stud
Externí odkaz:
https://doaj.org/article/04b8915d6d5c4bfeb5c82bcdf63fda7c
Autor:
Troy McSherry, Jeffrey R. Fishpaugh, Panfilo Ozaeta, Steven P. Allen, Kenton L. Longenecker, Jennifer McSherry, Carol Ramsay
Publikováno v:
mAbs. 8:718-725
Post-translational modifications can have a signification effect on antibody stability. A comprehensive approach is often required to best understand the underlying reasons the modification affects the antibody's potency or aggregation state. Monoclo
Autor:
Susan Madison-Antenucci, Kevin Y. Cheng, Kelly E. Coller, Susan J. Wong, Mark Pope, Thomas P. Leary, George J. Dawson, Christopher C. Marohnic, Alfredo R. Narvaez, Jeffrey R. Fishpaugh, M. Marcinkus, Phillip W. Schultz, Zachary A. Pfeiffer, Alak K. Kar, Janet M. Bergsma, Kathy Otis, Orlando H. Gumbs, James R. Fino, Randee R. Elsing
Publikováno v:
Journal of Clinical Microbiology
The tick-borne protozoan Babesia microti is responsible for more than 200 cases of transfusion-transmitted babesiosis (TTB) infection in the United States that have occurred over the last 30 years. Measures to mitigate the risk of TTB include nucleic
Publikováno v:
PROTEOMICS. 14:1311-1321
The absolute quantitation of the targeted protein using MS provides a promising method to evaluate/verify biomarkers used in clinical diagnostics. In this study, a cardiac biomarker, troponin I (TnI), was used as a model protein for method developmen
Publikováno v:
ELECTROPHORESIS. 34:825-832
Several method parameters have been refined for application of CIEF methods to provide optimal capillary robustness and performance longevity while maintaining desired analytical output for the ever increasing characterization scrutiny of protein rea
Autor:
Yon-Yih Chen, Rad K. Nair, Sushil D. Rege, Larissa Coombs Harwick, Gangamani S. Beligere, Jonathan Grote, Kevin R. Rupprecht, Jeffrey R. Fishpaugh, Zhen Lin
Publikováno v:
Analytical Biochemistry. 407:160-164
Dot-blot is a versatile and simple analysis to perform. We adapted this method as a simple identity test for monoclonal antibodies to a number of small compounds: three transplant drugs, an anticonvulsant, a steroid, an anticancer drug, and an antibi
Autor:
Tracey D. Rae, Jeffrey R. Fishpaugh, Ewa Z. Lang, Kevin R. Rupprecht, Svetoslava D. Gregory, Janet M. Bergsma
Publikováno v:
Analytical biochemistry. 486
Sodium dodecyl sulfate (SDS) is used to denature and solubilize proteins, especially membrane and other hydrophobic proteins. A quantitative method to determine the concentration of SDS using the dye Stains-All is known. However, this method lacks th
Publikováno v:
Organic Preparations and Procedures International. 34:326-331
Publikováno v:
Journal of Labelled Compounds and Radiopharmaceuticals. 43:909-915
We describe the synthesis of 3,3,4,4,3′,3′,4′,4′-homocystine-d82 in four steps. Compound 2 has been utilized as an internal standard for the LC-MS quantification of L-homocysteine 1, a marker for cardiac disease. 1,1,2,2-Tetradeutero-2-bromoe
Publikováno v:
Tetrahedron: Asymmetry. 10:4151-4156
Asymmetric syntheses of l -homocysteine 1 and l -homocystine 2 are described. Alkylation of the carbanion derived from Schollkopf reagent 3 and ensuing hydrolyses gave S -triphenylmethyl- l -homocysteine 6 . Removal of the triphenylmethyl group gave