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Autor:
Kenneth Dale Brady, Jeffrey P. Welch, Robert V. Talanian, John A. Mankovich, Luan C. Dang, Winnie W. Wong, Maria Hackett, Catherine R. Ferenz
Publikováno v:
Journal of Biological Chemistry. 271:21853-21858
We report the preparation and characterization of interleukin-1beta converting enzyme (ICE) refolded from its p20 and p10 protein fragments. Refolded ICE heterodimer (p20p10) was catalytically active but unstable, and in size exclusion chromatography