Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Jeffrey M. Spencer"'
Autor:
Gregory W. Goldberg, Jeffrey M. Spencer, David O. Giganti, Brendan R. Camellato, Neta Agmon, David M. Ichikawa, Jef D. Boeke, Marcus B. Noyes
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
The PAM specificity of SpCas9 can be altered with positive selection during directed evolution. Here the authors use simultaneous positive and negative selection to improve activity on NAG PAMs while reducing activity on NGG PAMs.
Externí odkaz:
https://doaj.org/article/3e675de09c284972a7413a9b4147d955
Autor:
David M. Ichikawa, Osama Abdin, Nader Alerasool, Manjunatha Kogenaru, April L. Mueller, Han Wen, David O. Giganti, Gregory W. Goldberg, Samantha Adams, Jeffrey M. Spencer, Rozita Razavi, Satra Nim, Hong Zheng, Courtney Gionco, Finnegan T. Clark, Alexey Strokach, Timothy R. Hughes, Timothee Lionnet, Mikko Taipale, Philip M. Kim, Marcus B. Noyes
Publikováno v:
Nature Biotechnology.
Cys2His2 zinc finger (ZF) domains engineered to bind specific target sequences in the genome provide an effective strategy for programmable regulation of gene expression, with many potential therapeutic applications. However, the structurally intrica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::078da30df1b51cdd076e7daf23ffe8ec
https://doi.org/10.1038/s41587-022-01624-4
https://doi.org/10.1038/s41587-022-01624-4
Autor:
Jef D. Boeke, Marcus B. Noyes, Brendan Camellato, David M. Ichikawa, Gregory W. Goldberg, Neta Agmon, Jeffrey M. Spencer, David Giganti
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Nature Communications
Nature Communications
The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacent motif (PAM) sequences, in addition to base-pairing interactions between its guide RNA and target D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25762a6571ede1ec0d9d9c2b6abd77b8
https://doaj.org/article/3e675de09c284972a7413a9b4147d955
https://doaj.org/article/3e675de09c284972a7413a9b4147d955
Autor:
Benny Abraham Kaipparettu, Xiaoliu Zhang, Sandi R. Wilkenfeld, Jeffrey M. Spencer, Kimal Rajapakshe, Efrosini Tsouko, Thomas L. Pulliam, Daniel E. Frigo, Fotis Nikolos, Chenchu Lin, Edwin Cheung, Dominik Awad, Sheiva S Vakili, Rajasekhara Reddy Katreddy, Cristian Coarfa, Mark A. White, Arun Sreekumar
Despite altered metabolism being an accepted hallmark of cancer, it is still not completely understood which signaling pathways regulate these processes. Given the central role of androgen receptor (AR) signaling in prostate cancer, we hypothesized t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e53f3210a4303e29cf56de684690d47f
https://europepmc.org/articles/PMC5831527/
https://europepmc.org/articles/PMC5831527/
Autor:
Jeffrey M. Spencer, Xiaoliu Zhang
Publikováno v:
Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
RNA-guided endonucleases (RGENs) have invigorated the field of site-specific nucleases. The success of Streptococcus pyogenes Cas9 (SpCas9) has led to the discovery of several other CRISPR-associated RGENs. As more RGENs become available, it will be
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09b181addcde084e4973152a67a3269d
https://pubmed.ncbi.nlm.nih.gov/29203891
https://pubmed.ncbi.nlm.nih.gov/29203891
575. Complete Removal of E-Cadherin from 4T-1 Murine Breast Cancer Cells Has No Effect on Metastasis
Publikováno v:
Molecular Therapy. 23
Currently accepted models for metastatic progression involve several steps. The first of these, local invasion of the primary tumor, requires detachment from the tumor mass and increased mobility. Epithelial to Mesenchymal Transition (EMT) is believe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e91f0c510d0c80dc80e6044012987a7d
Autor:
Jeffrey M. Spencer, Xiaoliu Zhang
Publikováno v:
Molecular Therapy. 23
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) bacterial immunity targets invading nucleic acid sequences using an RNA guide (gRNA). The well-defined Cas9 from Streptococcus pyogenes’ type II CRISPR system has been utilized as a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11ade3eb20fd437ca8b37878c924eb69
Autor:
Jeffrey M. Spencer, Xiaoliu Zhang
Publikováno v:
Molecular Therapy. 24:S57
CRISPR-Cas systems, RNA-guided endonucleases (RGENs), have been utilized for gene editing in a multitude of organisms. The robust editing ability of these nucleases promises to deliver novel therapeutics. CRISPR-derived therapeutics will benefit from
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c51f4cd8dcb96a21f166f69085bdb025
https://doi.org/10.1016/s1525-0016(16)32950-1
https://doi.org/10.1016/s1525-0016(16)32950-1
Autor:
Jeffrey M. Spencer
Every Christian doctrine has its day to be attacked – and defended. The Christian Church, from its conception, has believed in such magnificent teachings as the existence of a theistic God, the possibility of miracles, the uniqueness of Christ, the