Zobrazeno 1 - 10
of 42
pro vyhledávání: '"Jeffrey A, Pleiss"'
Autor:
Zachary W. Dwyer, Jeffrey A. Pleiss
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-5 (2023)
In this comment, the authors discuss the potentially widespread problem of selection bias in drawing biological conclusions from RNA sequencing data.
Externí odkaz:
https://doaj.org/article/770abe4034c34a1b9ca1e7144d0001c0
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 6, Iss 6, Pp 1513-1523 (2016)
Pre-mRNA splicing is an essential component of eukaryotic gene expression and is highly conserved from unicellular yeasts to humans. Here, we present the development and implementation of a sequencing-based reverse genetic screen designed to identify
Externí odkaz:
https://doaj.org/article/8b9f215065b940a9a61700f9a53b56c5
Autor:
Catalina Alfonso-Parra, Yasir H Ahmed-Braimah, Ethan C Degner, Frank W Avila, Susan M Villarreal, Jeffrey A Pleiss, Mariana F Wolfner, Laura C Harrington
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 10, Iss 2, p e0004451 (2016)
The Aedes aegypti mosquito is a significant public health threat, as it is the main vector of dengue and chikungunya viruses. Disease control efforts could be enhanced through reproductive manipulation of these vectors. Previous work has revealed a r
Externí odkaz:
https://doaj.org/article/7b17511d35414d1d89aa0a8ccee4a789
Publikováno v:
Molecular Cell. 82:2967-2981.e6
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e5a36b2be0053685473a5aa36ed47b69
https://doi.org/10.1016/j.molcel.2022.06.020
https://doi.org/10.1016/j.molcel.2022.06.020
Publikováno v:
Nature methods
Targeted RNA sequencing (RNA-seq) aims to focus coverage on areas of interest that are inadequately sampled in standard RNA-seq experiments. Here we present multiplexed primer extension sequencing (MPE-seq), an approach for targeted RNA-seq that uses
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e48b0e56484eb2631debaa45613d2dbf
https://doi.org/10.1038/s41592-018-0258-x
https://doi.org/10.1038/s41592-018-0258-x
SummaryUnderstanding how the spliceosome processes its composite of substrates through the two chemical steps required for mRNA production will be essential to deciphering splicing regulation and mis-regulation. Here we measure the in vivo rates of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::99446ee1651092bd89d87881256b089c
https://doi.org/10.1101/2020.10.13.338152
https://doi.org/10.1101/2020.10.13.338152
Publikováno v:
Molecular cell. 82(16)
We generate high-precision measurements of the in vivo rates of both chemical steps of pre-mRNA splicing across the genome-wide complement of substrates in yeast by coupling metabolic labeling, multiplexed primer-extension sequencing, and kinetic mod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4b7c68917a2b568d615d2a6270ba10c6
https://pubmed.ncbi.nlm.nih.gov/35830855
https://pubmed.ncbi.nlm.nih.gov/35830855
Autor:
Assaf Weiner, Hsiuyi V Chen, Chih Long Liu, Ayelet Rahat, Avital Klien, Luis Soares, Mohanram Gudipati, Jenna Pfeffner, Aviv Regev, Stephen Buratowski, Jeffrey A Pleiss, Nir Friedman, Oliver J Rando
Publikováno v:
PLoS Biology, Vol 10, Iss 7, p e1001369 (2012)
Packaging of eukaryotic genomes into chromatin has wide-ranging effects on gene transcription. Curiously, it is commonly observed that deletion of a global chromatin regulator affects expression of only a limited subset of genes bound to or modified
Externí odkaz:
https://doaj.org/article/7c4b159e3dc94410b76d427d20f40dac
Autor:
Laura-Oana Albulescu, Nevin Sabet, Mohanram Gudipati, Nicholas Stepankiw, Zane J Bergman, Tim C Huffaker, Jeffrey A Pleiss
Publikováno v:
PLoS Genetics, Vol 8, Iss 3, p e1002530 (2012)
Here we present the development and implementation of a genome-wide reverse genetic screen in the budding yeast, Saccharomyces cerevisiae, that couples high-throughput strain growth, robotic RNA isolation and cDNA synthesis, and quantitative PCR to a
Externí odkaz:
https://doaj.org/article/93b1f5a477324f42b8fcf6f0f117ea9c
Publikováno v:
PLoS Biology, Vol 5, Iss 4, p e90 (2007)
Appropriate expression of most eukaryotic genes requires the removal of introns from their pre-messenger RNAs (pre-mRNAs), a process catalyzed by the spliceosome. In higher eukaryotes a large family of auxiliary factors known as SR proteins can impro
Externí odkaz:
https://doaj.org/article/0f51f0ae2a3049649402aa71abc01c30