Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Jeff Wilkesman"'
Publikováno v:
Microplastics, Vol 3, Iss 1, Pp 46-66 (2024)
Given the broad and intense use of plastic, society is being increasingly affected by its degradation and by-products, particularly by microplastics (MPs), fragments smaller than 5 mm in size, and nanoplastics (NPs), with sizes less than 1 µm. MPs a
Externí odkaz:
https://doaj.org/article/c235190bced54e389bddd40ec501ad3a
Autor:
Gabriela Romero, Lellys M. Contreras, Carolina Aguirre Céspedes, Jeff Wilkesman, Josefa María Clemente-Jiménez, Felipe Rodríguez-Vico, Francisco Javier Las Heras-Vázquez
Publikováno v:
Polymers, Vol 15, Iss 15, p 3170 (2023)
The Y509E mutant of β-xylosidase from Geobacillus stearothermophilus (XynB2Y509E) (which also bears xylanase activity) has been immobilized in chitosan spheres through either entrapment or covalent bond formation methods. The maximum immobilization
Externí odkaz:
https://doaj.org/article/431a4fcc431d4bc395e0af6a1dc2200a
Autor:
Gabriela Romero, Lellys M. Contreras, Carolina Aguirre, Jeff Wilkesman, Josefa María Clemente-Jiménez, Felipe Rodríguez-Vico, Francisco Javier Las Heras-Vázquez
Publikováno v:
Molecules, Vol 26, Iss 2, p 451 (2021)
Cross-linked enzyme aggregates (CLEAs) of the Y509E mutant of glycoside hydrolase family 52 β-xylosidase from Geobacillus stearothermophilus with dual activity of β-xylosidase and xylanase (XynB2Y509E) were prepared. Ammonium sulfate was used as th
Externí odkaz:
https://doaj.org/article/5cd4b9a4223d4b9c8e0827b9802b2f4d
Publikováno v:
Environment, Development and Sustainability. 23:8252-8271
Manganese (Mn) contained in cellulose is partially responsible for an increased consumption of paper bleaching chemicals (like O2, H2O2), consequently diminishing the efficiency in pulp processing, darkening the pulp and deteriorating pulp quality. U
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f059e45aeccdc4c4f36af4284b5352e9
https://doi.org/10.1007/s10668-020-00962-0
https://doi.org/10.1007/s10668-020-00962-0
Autor:
Josefa María Clemente-Jiménez, Gabriela Romero, Felipe Rodríguez-Vico, Jeff Wilkesman, Lellys M. Contreras, Carolina Aguirre, Francisco Javier Las Heras-Vázquez
Publikováno v:
Molecules
Molecules, Vol 26, Iss 451, p 451 (2021)
Volume 26
Issue 2
Molecules, Vol 26, Iss 451, p 451 (2021)
Volume 26
Issue 2
Cross-linked enzyme aggregates (CLEAs) of the Y509E mutant of glycoside hydrolase family 52 &beta
xylosidase from Geobacillus stearothermophilus with dual activity of &beta
xylosidase and xylanase (XynB2Y509E) were prepared. Ammonium sulfat
xylosidase from Geobacillus stearothermophilus with dual activity of &beta
xylosidase and xylanase (XynB2Y509E) were prepared. Ammonium sulfat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08e0ba1ea6beb5371e765d2eef1b7260
http://europepmc.org/articles/PMC7830863
http://europepmc.org/articles/PMC7830863
Autor:
María, Tapizquent, Marleny, Fernández, Georgina, Barreto, Zully, Hernández, Lellys M, Contreras, Liliana, Kurz, Jeff, Wilkesman
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1626
Lipases are esterases that occur widely in nature, yet those with commercial relevance are exclusively from microbial origin. Glycerol and long-chain fatty acids are the products after hydrolysis of esters bonds in saponifiable lipids catalyzed by li
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7aa787db8f58485a579cbb2ac7692c61
https://pubmed.ncbi.nlm.nih.gov/28608222
https://pubmed.ncbi.nlm.nih.gov/28608222
Autor:
Oscar, González, Jeff, Wilkesman
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1626
Electrophoresis under denaturing conditions in the presence of SDS is a standard method for the protein and enzyme scientist. Nevertheless, there are special situations where this method may originate nonoptimal results. SDS may cause protein aggrega
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::988484f4b7f010fdf86aba0638bc2405
https://pubmed.ncbi.nlm.nih.gov/28608221
https://pubmed.ncbi.nlm.nih.gov/28608221
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1626
It is possible to analyze peroxidase (POD) from different vegetable sources by electrophoresis. Zymography, i.e., a SDS-PAGE method to detect enzyme activity, is used to specifically detect POD activity and to visualize the total protein profile. For
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::38dc28dde2a2a38b66791e5700040a3f
https://pubmed.ncbi.nlm.nih.gov/28608212
https://pubmed.ncbi.nlm.nih.gov/28608212
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1626
Lipase and protease present in cell-free fractions of thermophilic Bacillus sp. cultures were analyzed by polyacrylamide gel (PAG) electrophoresis. After run, the gel is electrotransferred to another PAG copolymerized with glycerol tributyrate, olive
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f0d340738368f59d3580fcc0b862bf04
https://pubmed.ncbi.nlm.nih.gov/28608219
https://pubmed.ncbi.nlm.nih.gov/28608219
Autor:
Jeff, Wilkesman, Liliana, Kurz
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1626
Zymography, the detection, identification, and even quantification of enzyme activity fractionated by gel electrophoresis, has received increasing attention in the last years, as revealed by the number of articles published. A number of enzymes are r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2eb646344c216f5ef41723bd8bb1780c
https://pubmed.ncbi.nlm.nih.gov/28608195
https://pubmed.ncbi.nlm.nih.gov/28608195