Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Jeff H. Robinson"'
Publikováno v:
Thrombosis Research. 59:687-692
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f71c8b00fecd4ed37d97f4b133488c8
https://doi.org/10.1016/0049-3848(90)90429-g
https://doi.org/10.1016/0049-3848(90)90429-g
Publikováno v:
Thrombosis and Haemostasis. 63:459-463
SummaryA hybrid plasminogen activator consisting of the “A” chain of plasmin linked to the “B” chain of rt-PA was inhibited in vitro in human and guinea pig plasmas 4 to 5-fold more rapidly than its parent activator, two-chain t-PA. Using zym
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7037b891c19b9a3528c1d1bcd9207615
https://doi.org/10.1055/s-0038-1645066
https://doi.org/10.1055/s-0038-1645066
Autor:
Martin C. Coldwell, Jeff H. Robinson, Stuart Ball, Ted Cook, Izzy Boyfield, Mark McHale, Israel S. Gloger, Nicole Herrity, Fiona M. Winn
Publikováno v:
FEBS letters. 345(2-3)
A synthetic version of the human D4 (hD4) dopamine receptor was prepared. The G/C content of the natural gene was reduced by 14% without altering the amino acid composition of the corresponding protein sequence. HEK293 cells were transfected with the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b3b0773b6eb720d1b036cb370179132
https://pubmed.ncbi.nlm.nih.gov/8200447
https://pubmed.ncbi.nlm.nih.gov/8200447
Autor:
Ian B. Dodd, David Cronk, Conrad Gerald Chapman, Shelagh Wilson, Jeff H. Robinson, Michael J. Browne, Chris Hastwell
Publikováno v:
Biologicals : journal of the International Association of Biological Standardization. 21(3)
Abstract. Plasmids encoding tissue-type plasminogen activators lacking residues K296-G302 were constructed and were expressed in a Hela cell transient expression system. Conditioned media from the cultures were tested in a number of systems designed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4e35359fd33885fb8d4fd33a1340ab3
https://pubmed.ncbi.nlm.nih.gov/8117440
https://pubmed.ncbi.nlm.nih.gov/8117440
Autor:
Jeff H. Robinson, Michael J. Browne
Publikováno v:
Trends in biotechnology. 9(3)
Attempts are being made to redesign the structure of tissue-type plasminogen activator (t-PA) in order to increase its plasma half-life, increase its fibrin affinity or decrease its rate of interaction with plasma inhibitors. The principal strategies
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ce4a2b18c5c850f8d4356a549562716
https://pubmed.ncbi.nlm.nih.gov/1367504
https://pubmed.ncbi.nlm.nih.gov/1367504
Publikováno v:
Thrombosis Research. 40:693-702
Human embryonic lung fibroblast (MRC5) cells secreted small amounts of plasminogen activators into normal, serum-free harvest medium. Stimulation with calcium led to markedly enhanced levels of activator. The major species of plasminogen activator in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c26f459b0f5951669d4338ebacd07b2
https://doi.org/10.1016/0049-3848(85)90306-8
https://doi.org/10.1016/0049-3848(85)90306-8
Publikováno v:
Thrombosis and Haemostasis. 59:523-528
SummaryPurified preparations of recombinant tissue-type plasminogen activator (t-PA) from the recombinant Bowes melanoma cell line TRBM6 were shown to contain multiple species of plasminogen activator. Using a combination of chromatography on Sephade
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a4769c6c14197d0f4d7cfa03b22a26d
https://doi.org/10.1055/s-0038-1647527
https://doi.org/10.1055/s-0038-1647527
Publikováno v:
Thrombosis and Haemostasis. 54:422-424
SummaryExtra copies of the human tissue-type plasminogen activator (t-PA) gene were introduced into the Bowes melanoma cell line. We obtained a recombinant cell line (TRBM6) which secretes approximately ten-fold more t-PA than the parent cell line. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3b4e1ee6f297729f2ae2c1ea5257f884
https://doi.org/10.1055/s-0038-1657864
https://doi.org/10.1055/s-0038-1657864
Autor:
Jeff H. Robinson
Publikováno v:
Thrombosis research. 33(2)
The human diploid fibroblast cell line, MRC-5, derived from embryo lung tissue produced only small quantities of plasminogen activator (PA) when harvested using a standard nutrient medium (Eagle's Minimal Essential Medium, MEM). Use of a schedule des
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9463001d21b7cc14f44d88d704c9dbf
https://pubmed.ncbi.nlm.nih.gov/6538347
https://pubmed.ncbi.nlm.nih.gov/6538347