Rotavirus-Induced Lipid Droplet Biogenesis Is Critical for Virus Replication

Autor: Jeanette M. Criglar, Mary K. Estes, Sue E. Crawford

Publikováno v: Frontiers in Physiology, Vol 13 (2022)

A variety of pathogens, including viruses, bacteria and parasites, target cellular lipid droplets for their replication. Rotaviruses (RVs) infect the villous epithelium of the small intestine and are a major cause of acute gastroenteritis in infants

Externí odkaz: https://doaj.org/article/512267c6aa7647a09a1f6428d2991ca0

A Genetically Engineered Rotavirus NSP2 Phosphorylation Mutant Impaired in Viroplasm Formation and Replication Shows an Early Interaction between vNSP2 and Cellular Lipid Droplets

Autor: Jeanette M. Criglar, Hunter G. Smith, Boyang Zhao, Mary K. Estes, Fabio Stossi, Sue E. Crawford

Publikováno v: J Virol

Many RNA viruses replicate in cytoplasmic compartments (virus factories or viroplasms) composed of viral and cellular proteins, but the mechanisms required for their formation remain largely unknown. Rotavirus (RV) replication in viroplasms requires

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f47146dba42d2291dd543ddf1bd4c58b
https://doi.org/10.1128/jvi.00972-20

Rotavirus Calcium Dysregulation Manifests as Dynamic Calcium Signaling in the Cytoplasm and Endoplasmic Reticulum

Autor: Jeanette M. Criglar, Joseph M. Hyser, Alexandra L. Chang-Graham, Mary K. Estes, Alicia C. Strtak, Nina K. Ramachandran, John T. Patton, Jacob L. Perry, Asha A. Philip

Publikováno v: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-20 (2019)

Like many viruses, rotavirus (RV) dysregulates calcium homeostasis by elevating cytosolic calcium ([Ca2+]cyt) and decreasing endoplasmic reticulum (ER) stores. While an overall, monophasic increase in [Ca2+]cyt during RV infection has been shown, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e277b6ca9fb187ce9c2164abcebdf03
http://europepmc.org/articles/PMC6658527

Phosphorylation cascade regulates the formation and maturation of rotaviral replication factories

Autor: B. V. V. Prasad, Jeanette M. Criglar, Ramakrishnan Anish, Mary K. Estes, Banumathi Sankaran, Sue E. Crawford, Liya Hu

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, vol 115, iss 51
Proceedings of the National Academy of Sciences

The rotavirus (RV) genome is replicated and packaged into virus progeny in cytoplasmic inclusions called viroplasms, which require interactions between RV nonstructural proteins NSP2 and NSP5. How viroplasms form remains unknown. We previously found

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::581c75e76e6136c9c63534a3554845c0
https://escholarship.org/uc/item/3cc030fd

Reconciliation of Rotavirus Temperature-Sensitive Mutant Collections and Assignment of Reassortment Groups D, J, and K to Genome Segments

Autor: Harry B. Greenberg, Robert F. Ramig, Mary K. Estes, Jeanette M. Criglar

Publikováno v: Journal of Virology. 85:5048-5060

Four rotavirus SA11 temperature-sensitive ( ts ) mutants and seven rotavirus RRV ts mutants, isolated at the National Institutes of Health (NIH) and not genetically characterized, were assigned to reassortment groups by pairwise crosses with the SA11

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1158bc01865566d980e40dca6215d111
https://doi.org/10.1128/jvi.02691-10