Cationic Oligospermine-Oligonucleotide Conjugates Provide Carrier-free Splice Switching in Monolayer Cells and Spheroids

Autor: Marc Nothisen, Phanélie Perche-Létuvée, Jean-Paul Behr, Jean-Serge Remy, Mitsuharu Kotera

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 13, Iss , Pp 483-492 (2018)

We report the evaluation of 18-mer 2′-O-methyl-modified ribose oligonucleotides with a full-length phosphorothioate backbone chemically conjugated at the 5′ end to the oligospermine units (Sn-: n = 5, 15, 20, 25, and 30 [number of spermine units]

Externí odkaz: https://doaj.org/article/c3f230b50ab44ad0bb3ef0b0af083011

Cationic Oligospermine-Oligonucleotide Conjugates Provide Carrier-free Splice Switching in Monolayer Cells and Spheroids

Autor: Jean-Paul Behr, Mitsuharu Kotera, Jean-Serge Remy, Phanélie Perche-Létuvée, Marc Nothisen

Publikováno v: Molecular Therapy: Nucleic Acids, Vol 13, Iss, Pp 483-492 (2018)
Molecular Therapy-Nucleic Acids
Molecular Therapy-Nucleic Acids, Elsevier, 2018, 13, pp.483-492. ⟨10.1016/j.omtn.2018.09.027⟩
Molecular Therapy. Nucleic Acids

We report the evaluation of 18-mer 2′-O-methyl-modified ribose oligonucleotides with a full-length phosphorothioate backbone chemically conjugated at the 5′ end to the oligospermine units (Sn-: n = 5, 15, 20, 25, and 30 [number of spermine units]

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fd72c1b5dca8ee1fe4ae3da4f73c27a
http://www.sciencedirect.com/science/article/pii/S2162253118302725

Structure Tuning of Cationic Oligospermine–siRNA Conjugates for Carrier-Free Gene Silencing

Autor: Jean-Paul Behr, Jean-Serge Remy, Mitsuharu Kotera, Marc Nothisen, Jérémy Bagilet

Publikováno v: Molecular Pharmaceutics
Molecular Pharmaceutics, American Chemical Society, 2016, 13 (8), pp.2718-2728. ⟨10.1021/acs.molpharmaceut.6b00309⟩

Oligospermine-siRNA conjugates are able to induce efficient luciferase gene silencing upon carrier-free transfection. These conjugates are readily accessible by a versatile automated chemistry that we developed using a DMT-spermine phosphoramidite re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b5f2f4dde5df46e35a0c2be93aa6079
https://doi.org/10.1021/acs.molpharmaceut.6b00309

Cell-penetrating cationic siRNA and lipophilic derivatives efficient at nanomolar concentrations in the presence of serum and albumin

Autor: Jean-Paul Behr, Marc Nothisen, Jean-Serge Remy, Mitsuharu Kotera, Jérémy Bagilet, Phanélie Perche

Publikováno v: Journal of Controlled Release. 170:92-98

Despite its considerable interest in human therapy, in vivo siRNA delivery is still suffering from hurdles of vectorization. We have shown recently efficient gene silencing by non-vectorized cationic siRNA. Here, we describe the synthesis and in vitr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a023a67c3d862aadb3c98f665f84cfd
https://doi.org/10.1016/j.jconrel.2013.04.013

Sticky overhangs enhance siRNA-mediated gene silencing

Autor: Patrick Erbacher, Anne-Laure Bolcato-Bellemin, Marie-Elise Bonnet, Jean-Paul Behr, Gaëlle Creusat

Publikováno v: Proceedings of the National Academy of Sciences. 104:16050-16055

siRNA delivery to cells offers a convenient and powerful means of gene silencing that bypasses several barriers met by gene delivery. However, nonviral vectors, and especially polymers, form looser complexes with siRNA than with plasmid DNA. As a con

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd6c2f6922ac1fdfc42c2663e5661ad9
https://doi.org/10.1073/pnas.0707831104