Mechanisms and Regulation of Nonsense-Mediated mRNA Decay and Nonsense-Associated Altered Splicing in Lymphocytes

Autor: Jean-Marie Lambert, Mohamad Omar Ashi, Nivine Srour, Laurent Delpy, Jérôme Saulière

Publikováno v: International Journal of Molecular Sciences, Vol 21, Iss 4, p 1335 (2020)

The presence of premature termination codons (PTCs) in transcripts is dangerous for the cell as they encode potentially deleterious truncated proteins that can act with dominant-negative or gain-of-function effects. To avoid the synthesis of these sh

Externí odkaz: https://doaj.org/article/9d7b18d9b8c24e2e90af8370fa10331a

Splice switching oligonucleotide mediated gene knockdown in B cells and plasma cells

Autor: Nathalie Faumont, Anne Marchalot, Jean-Marie Lambert, François Boyer, Laurent Delpy, Jean Feuillard, Justine Pollet, Jeanne Cook-Moreau

The need to identify new therapeutic approaches to the treatment of cancers of the B lymphoid lineage is crucial. Unlike CRISPR/Cas technology, antisense strategies result in transient modifications of gene expression and lack mutagenic effects at th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7fcbccd09f7ac75b7184a19a389ed0de
https://doi.org/10.1101/2020.09.18.302984

Uncoupling Splicing From Transcription Using Antisense Oligonucleotides Reveals a Dual Role for I Exon Donor Splice Sites in Antibody Class Switching

Autor: Jean-Marie Lambert, Mohamad Omar Ashi, Laurent Delpy, Anne Marchalot, Sandrine Lecardeur, Nivine Srour, Soazig Le Pennec, Claire Carrion

Publikováno v: Frontiers in Immunology
Frontiers in Immunology, Vol 11 (2020)

Class switch recombination (CSR) changes antibody isotype by replacing Cμ constant exons with different constant exons located downstream on the immunoglobulin heavy (IgH) locus. During CSR, transcription through specific switch (S) regions and proc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2360456d660dd79f22594b84563ed307

Uncoupling splicing from transcription using antisense oligonucleotides reveals a dual role for I exon donor splice sites in antibody class switching

Autor: Anne Marchalot, Soazig Le Pennec, Jean-Marie Lambert, Mohamad Omar Ashi, Nivine Srour, Laurent Delpy

Class switch recombination (CSR) changes antibody isotype by replacing Cμ constant exons with different constant exons located downstream on the immunoglobulin heavy (IgH) locus. During CSR, transcription through specific switch (S) regions and proc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f18126dbf0620dbc90e114e74e87c00d
https://doi.org/10.1101/850867

Physiological and druggable skipping of immunoglobulin variable exons in plasma cells

Autor: Michel Cogné, Nivine Srour, Mohamad Omar Ashi, Eric Pinaud, Sandrine Le Noir, Ophélie Martin, Christophe Sirac, Jérôme Saulière, Anne Marchalot, Maria Victoria Ayala, Jérôme Moreaux, Jean-Marie Lambert, Laurent Delpy

Publikováno v: Cellular and molecular immunology
Cellular and molecular immunology, Nature Publishing Group/Chinese Society of Immunology, 2018, ⟨10.1038/s41423-018-0160-6⟩
Cellular and molecular immunology, Nature Publishing Group/Chinese Society of Immunology, 2019, 16 (10), pp.810-819. ⟨10.1038/s41423-018-0160-6⟩
Cell Mol Immunol

International audience; The error-prone V(D)J recombination process generates considerable amounts of nonproductive immunoglobulin (Ig) pre-mRNAs. We recently demonstrated that aberrant Ig chains lacking variable (V) domains can be produced after non

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b17d7f7cec226ba8dcc7c62c1e6681e9
https://hal.archives-ouvertes.fr/hal-02048156