Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex

Autor: Marie Glavier, Dhenesh Puvanendran, Dimitri Salvador, Marion Decossas, Gilles Phan, Cyril Garnier, Elisa Frezza, Quentin Cece, Guy Schoehn, Martin Picard, Jean-Christophe Taveau, Laetitia Daury, Isabelle Broutin, Olivier Lambert

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)

The tripartite multidrug efflux system MexAB-OprM is a major actor in Pseudomonas aeruginosa antibiotic resistance by exporting a large variety of antimicrobial compounds. Here authors present cryo-EM structures of MexB at various stages of the assem

Externí odkaz: https://doaj.org/article/45b848677d664bb2a4cbada3e29d4ee0

Tripartite assembly of RND multidrug efflux pumps

Autor: Laetitia Daury, François Orange, Jean-Christophe Taveau, Alice Verchère, Laura Monlezun, Céline Gounou, Ravi K. R. Marreddy, Martin Picard, Isabelle Broutin, Klaas M. Pos, Olivier Lambert

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-8 (2016)

Tripartite efflux systems consist of inner membrane, outer membrane and periplasmic components. Here, Daury et al. reconstitute native versions of RND transporters in nanodiscs and present projection structures emphasizing the role of the periplasmic

Externí odkaz: https://doaj.org/article/eba793de8123457e92c40e3142a2ad4f

Minimal nanodisc without exogenous lipids for stabilizing membrane proteins in detergent-free buffer

Autor: Sébastien Mongrand, Cyril Garnier, Marion Decossas, Olivier Lambert, Sophie Lecomte, Jean Christophe Taveau, Gilles Phan, Guy Schoehn, Marie Glavier, Isabelle Broutin, Dimitri Salvador, Laetitia Daury

Publikováno v: Biochimica et Biophysica Acta:Biomembranes
Biochimica et Biophysica Acta:Biomembranes, Elsevier, In press, 1861 (4), pp.852-860. ⟨10.1016/j.bbamem.2019.01.013⟩
Biochimica et Biophysica Acta:Biomembranes, Elsevier, 2019, 1861 (4), pp.852-860. ⟨10.1016/j.bbamem.2019.01.013⟩
Biochimica et Biophysica Acta:Biomembranes, Elsevier, 2019, 1861 (4), pp.852-860
Biochimica et Biophysica Acta:Biomembranes, 2019, 1861 (4), pp.852-860. ⟨10.1016/j.bbamem.2019.01.013⟩

International audience; Membrane protein stabilization after detergent solubilization presents drawbacks for structural and biophysical studies, in particular that of a reduced stability in detergent micelles. Therefore, alternative methods are requi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::814f87554b369ed2209121988e152732
http://hdl.handle.net/20.500.12278/109291

Single lipoaminoglycoside promotes efficient intracellular antibody delivery: A comprehensive insight into the mechanism of action

Autor: Sophie Lecomte, Bruno Pitard, Jean-Christophe Taveau, Olivier Lambert, Sylvie Poussard, Laurence Dallet, Marion Decossas

Publikováno v: Nanomedicine: Nanotechnology, Biology and Medicine
Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2018, 14 (1), pp.141-151
Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2018, 14 (1), pp.141-151. ⟨10.1016/j.nano.2017.09.005⟩
Nanomedicine: Nanotechnology, Biology and Medicine, 2018, 14 (1), pp.141-151. ⟨10.1016/j.nano.2017.09.005⟩

Delivery of biologically active proteins into cells is emerging as important strategy for many applications. Previous experiments have shown that lipoaminoglycosides were capable of delivery of the anti-cytokeratin8 antibody (anti-K8) but only when f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23d4fabf10e4e049ef909fb2396f494c
https://hal.archives-ouvertes.fr/hal-03184449

Nitroxide-Mediated Polymerization-Induced Self-Assembly of Block Copolymers at the Surface of Silica Particles: Toward New Hybrid Morphologies

Autor: Muriel Lansalot, X. G. Qiao, Pierre-Yves Dugas, Elodie Bourgeat-Lami, B. Charleux, Jean-Christophe Taveau, Olivier Lambert

Publikováno v: Macromolecules
Macromolecules, American Chemical Society, 2017, 50 (10), pp.3796-3806. ⟨10.1021/acs.macromol.7b00033⟩

Silica/polymer latexes with concentric core–shell–corona, half-capped, snowman-like vesicular, tadpole- and centipede-like morphologies have been produced by nitroxide-mediated polymerization-induced self-assembly (PISA) performed in the presence

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a8af8bd9d54abfc1f3258cf76ca94fd
https://hal.archives-ouvertes.fr/hal-01716039/document