Zobrazeno 1 - 4
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pro vyhledávání: '"Jean-Bernard Guillaume"'
Autor:
J. Courtois, J.-C. Joncquiert, Max Béchet, Y. Tierny, H.-C. Dubourguier, Jean-Bernard Guillaume
Publikováno v:
FEMS Microbiology Letters. 84:273-278
A genomic bank was constructed in Escherichia coli HB101, consisting of DNA fragments from Bacteroides thetaiotaomicron strain 489 inserted within the vector pBR322. By screening on complex medium containing blue dextran, 10 stable dextranase-positiv
Publikováno v:
FEMS Microbiology Letters. 55:15-22
An Escherichia coli-Bacteroides shuttle vehicle (pKBF367-1) was constructed by combining the pBR322 derivative pKC7 (5.9 kb) with [1] a 4.6 kb cryptic plasmid from Bacteroides fragilis; and [2] the 4.2 kb EcoRI-B fragment of the B. fragilis plasmid p
Publikováno v:
Canadian Journal of Microbiology. 23:1178-1181
The beta-galactosidase (EC 3.2.1.23) activities of wild-type Rhizobium meliloti and its lactose slow-hydrolyzing mutant have been compared. The properties of the enzyme are very different in each strain. These differences allow us to prove that two e
Publikováno v:
Canadian Journal of Microbiology. 30:507-511
Using a suicide plasmid as a vector, the kanamycin–neomycin resistance conferring transposon Tn5 was introduced into the genome of the two Rhizobium meliloti strains 444 and M28str. Among clones carrying random Tn5 insertions, about 0.3% auxotrophi