Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Jean N. Buskin"'
Publikováno v:
BioTechniques, Vol 20, Iss 1, Pp 92-100 (1996)
Many aspects of physiology and gene regulation can be studied by examining the levels of enzymes harvested from cultured cells. We found that the yield from cultured cells of two different cytosolic enzymes, creatine kinase and the common reporter ge
Externí odkaz:
https://doaj.org/article/d769cc5ec1074be2888dc4b524c5109b
Publikováno v:
Developmental Dynamics. 235:2122-2133
Mouse P19 embryonal carcinoma cells undergo cardiogenesis in response to high density and DMSO. We have derived a clonal subline which undergoes cardiogenesis in response to high density, but without requiring exposure to DMSO. The new subline retain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::570d3adf30b6ec8488f0a1a9b6cb1bf6
https://doi.org/10.1002/dvdy.20863
https://doi.org/10.1002/dvdy.20863
Publikováno v:
Transgenic Research. 12:337-349
The muscle creatine kinase (MCK) gene is expressed at high levels only in differentiated skeletal and cardiac muscle. The activity of the cloned enhancer-promoter has previously been shown to be dependent on the Trex element which is specifically bou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::46e6106ff1584bad301a71fc96ca35df
https://doi.org/10.1023/a:1023369225799
https://doi.org/10.1023/a:1023369225799
Publikováno v:
The Journal of Nutritional Biochemistry. 7:670-676
Previous studies have indicated that a lack of available zinc inhibited myoblast differentiation as shown by a failure of the cells to fuse and low expression of creatine kinase mRNA and activity. However, the nature of the requirement for zinc and i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a9b679b6f0ec5e7e9fe5d5e99a410f04
https://doi.org/10.1016/s0955-2863(96)00129-5
https://doi.org/10.1016/s0955-2863(96)00129-5
Autor:
Margaret A. Shield, Harald S. Haugen, Christopher H. Clegg, Stephen D. Hauschka, Jean N. Buskin, Dorit B. Donoviel
Publikováno v:
Molecular and Cellular Biology. 16:1649-1658
Regulatory regions of the mouse muscle creatine kinase (MCK) gene, previously discovered by analysis in cultured muscle cells, were analyzed in transgenic mice. The 206-bp MCK enhancer at nt-1256 was required for high-level expression of MCK-chloramp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2987d8f98eecd4d339818e7658b68aa6
https://doi.org/10.1128/mcb.16.4.1649
https://doi.org/10.1128/mcb.16.4.1649
Publikováno v:
BioTechniques. 20:92-100
Many aspects of physiology and gene regulation can be studied by examining the levels of enzymes harvested from cultured cells. We found that the yield from cultured cells of two different cytosolic enzymes, creatine kinase and the common reporter ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bef490e86d8078ff175d4e4776ab1e99
https://doi.org/10.2144/96201st04
https://doi.org/10.2144/96201st04
Publikováno v:
Molecular and Cellular Biology. 13:2753-2764
We have used transient transfections in MM14 skeletal muscle cells, newborn rat primary ventricular myocardiocytes, and nonmuscle cells to characterize regulatory elements of the mouse muscle creatine kinase (MCK) gene. Deletion analysis of MCK 5'-fl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6464809cfabc046f82a1ace3ca010a38
https://doi.org/10.1128/mcb.13.5.2753
https://doi.org/10.1128/mcb.13.5.2753
Publikováno v:
The Journal of biological chemistry. 278(47)
The 1256-base pair enhancer-promoter of the mouse muscle creatine kinase gene includes three CAnnTG E-boxes that are conserved among mammals and have flanking and middle sequences conforming to consensus muscle regulatory factor binding sites. This s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6a103d68b392a7875bc9c6e0bd54d5f
https://pubmed.ncbi.nlm.nih.gov/12968024
https://pubmed.ncbi.nlm.nih.gov/12968024
Autor:
Quynh-Giao V, Nguyen, Jean N, Buskin, Charis L, Himeda, Christine, Fabre-Suver, Stephen D, Hauschka
Publikováno v:
Transgenic research. 12(3)
The muscle creatine kinase (MCK) gene is expressed at high levels only in differentiated skeletal and cardiac muscle. The activity of the cloned enhancer-promoter has previously been shown to be dependent on the Trex element which is specifically bou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::edeb8728aa7b2637e55d4e0c5e3f9b4f
https://pubmed.ncbi.nlm.nih.gov/12779122
https://pubmed.ncbi.nlm.nih.gov/12779122
Autor:
Dennis J. Hartigan-O'Connor, Steve Apone, Jean N. Buskin, Stephen D. Hauschka, Jeffrey S. Chamberlain, Ann S. Robinson, Dee Ann Williams-Gregory, Michael A. Hauser, Christopher J. Kirk, Stephen F. Hardy
Publikováno v:
Molecular therapy : the journal of the American Society of Gene Therapy. 2(1)
Adenoviral gene transfer holds promise for gene therapy, but effective transduction of a large and distributed tissue such as muscle will almost certainly require systemic delivery. In this context, the use of muscle-specific regulatory elements such
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56f11281f19010184559299aa27ecb69
https://pubmed.ncbi.nlm.nih.gov/10899824
https://pubmed.ncbi.nlm.nih.gov/10899824