Zobrazeno 1 - 10
of 53
pro vyhledávání: '"Jean Mauchamp"'
Publikováno v:
Biology of the Cell. 90:369-380
The thyroid follicle, the morphofunctional unit of thyroid gland, is a spheroidal structure formed by a monolayer of polarized cells surrounding a closed cavity in which thyroglobulin accumulates. Newly isolated porcine thyroid cells reorganize into
Publikováno v:
Journal of Endocrinology. 128:51-61
Thyroid cells cultured as monolayers on the porous bottom of culture chambers have been shown to express some specific functions of thyroid follicles. This system, which allows independent access to apical and basal media, is suitable for the long-te
Publikováno v:
Journal of Cellular Physiology. 144:354-364
Epithelial thyroid cells in primary cultures loaded with BCECF/AM rapidly released the impermeant fluorescent dye BCECF (bis(carboxyethyl)carboxyfluorescein) in the incubation medium. Cells organized into follicles rapidly cleared BCECF (80% within 1
Publikováno v:
Biology of the Cell
Biology of the Cell, Wiley, 1999, 91 (2), pp.143-155. ⟨10.1016/s0248-4900(99)80038-0⟩
Biology of the Cell, Wiley, 1999, 91 (2), pp.143-155. ⟨10.1016/s0248-4900(99)80038-0⟩
When cultured in high glucose containing medium, the human colon carcinoma cell line HT29-D4 and a clone derived by transfection with the MDR1 cDNA (MDR31) form multilayers of unorganized cells which are not polarized and are linked by desmosomes. Wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb0d9ae7b4b55121a7123b65d1c2a57b
https://hal-amu.archives-ouvertes.fr/hal-03128143
https://hal-amu.archives-ouvertes.fr/hal-03128143
Autor:
Jean Mauchamp, Olivier Delézay, Bernard Verrier, Jacques Fantini, Jurphaas Van Rietschoten, Kamel Mabrouk, Corinne Gerard
Publikováno v:
Journal of cellular physiology. 163(1)
In this study, we have characterized the Na/glucose transporter in polarized monolayers formed by the clonal human colon carcinoma cell line HT-29-D4. Isotopic tracer flux measurements show that differentiated HT-29-D4 cells possess a sodium-dependen
Publikováno v:
Molecular and cellular endocrinology. 106(1-2)
This report describes chloride and iodide effluxes across the basolateral membrane of porcine thyroid follicles reconstituted in culture. Basolateral chloride efflux is activated by thyrotropin (TSH). TSH (10 mU/ml) induces a twofold increase in the
Publikováno v:
Experimental cell research. 200(2)
When cultured in suspension, epithelial thyroid cells organized into inside-out follicles. We studied the behavior of these structures after seeding on polystyrene, type I collagen, and reconstituted basement membrane (RBM) gel. When seeded on plasti
Publikováno v:
Molecular and cellular endocrinology. 81(1-3)
Phenol red, commonly used as a pH indicator in tissue culture media, is known to possess estrogenic properties. We investigated the effect of phenol red on the process of thyroglobulin iodination which occurs only at the apical surface of porcine thy
Autor:
Jean Mauchamp, Jacques Pichon, Pierre Pic, Bernard Verrier, Jacques Marvaldi, Cécile Robert, Jacques Fantini
Publikováno v:
Experimental cell research. 189(1)
The clonal cell line HT29-D4 was able to grow in a completely defined medium containing EGF, selenous acid, and transferrin in the presence of the anti-helminthic drug suramin. In the absence of suramin, the kinetics of cell growth and the cell densi