Blocking late stages of splicing quickly limits pre-spliceosome assembly in vivo

Autor: Gonzalo I. Mendoza-Ochoa, J. David Barrass, Isabella E. Maudlin, Jean D. Beggs

Publikováno v: RNA Biology, Vol 16, Iss 12, Pp 1775-1784 (2024)

Pre-messenger RNA splicing involves multi-step assembly of the large spliceosome complexes that catalyse the two consecutive trans-esterification reactions, resulting in intron removal. There is evidence that proof-reading mechanisms monitor the fide

Externí odkaz: https://doaj.org/article/fe41cc9df50f45d7853c0dfdf966cbe4

Conditional depletion of transcriptional kinases Ctk1 and Bur1 and effects on co-transcriptional spliceosome assembly and pre-mRNA splicing

Autor: Isabella E. Maudlin, Jean D. Beggs

Publikováno v: RNA Biology
article-version (VoR) Version of Record

From yeast to humans, pre-mRNA splicing occurs mainly co-transcriptionally, with splicing and transcription functionally coupled such that they influence one another. The recruitment model of co-transcriptional splicing proposes that core members of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b4dbc409db442bfa2ef63d422b6baea
https://doi.org/10.1080/15476286.2021.1991673

Blocking late stages of splicing quickly limits pre-spliceosome assembly in vivo

Autor: J. David Barrass, Isabella E. Maudlin, Jean D. Beggs, Gonzalo I Mendoza-Ochoa

Publikováno v: Mendoza ochoa, G, Barrass, J, Maudlin, I & Beggs, J 2019, ' Blocking late stages of splicing quickly limits pre-spliceosome assembly in vivo ', Rna biology . https://doi.org/10.1080/15476286.2019.1657788
RNA Biology

Pre-messenger RNA splicing involves multi-step assembly of the large spliceosome complexes that catalyse the two consecutive trans-esterification reactions, resulting in intron removal. There is evidence that proof-reading mechanisms monitor the fide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04713d11e26e323785483db0b2b76b7c
https://doi.org/10.1080/15476286.2019.1657788

Modelling reveals kinetic advantages of co-transcriptional splicing.

Autor: Stuart Aitken, Ross D Alexander, Jean D Beggs

Publikováno v: PLoS Computational Biology, Vol 7, Iss 10, p e1002215 (2011)

Messenger RNA splicing is an essential and complex process for the removal of intron sequences. Whereas the composition of the splicing machinery is mostly known, the kinetics of splicing, the catalytic activity of splicing factors and the interdepen

Externí odkaz: https://doaj.org/article/ab4f106c25b3492fb8cc3da805ade245

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

Autor: J David, Barrass, Jean D, Beggs

Publikováno v: Journal of visualized experiments : JoVE. (150)

The nucleotide analogue, 4-thiouracil (4tU), is readily taken up by cells and incorporated into RNA as it is transcribed in vivo, allowing isolation of the RNA produced during a brief period of labelling. This is done by attaching a biotin moiety to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e92f073525c793c191dfe3fe90146ac7
https://pubmed.ncbi.nlm.nih.gov/31498317