Synthesis and Structure–Activity Relationship Studies of Pyrido [1,2-e]Purine-2,4(1H,3H)-Dione Derivatives Targeting Flavin-Dependent Thymidylate Synthase in Mycobacterium tuberculosis

Autor: Nicolas G. Biteau, Vincent Roy, Cyril Nicolas, Hubert F. Becker, Jean-Christophe Lambry, Hannu Myllykallio, Luigi A. Agrofoglio

Publikováno v: Molecules, Vol 27, Iss 19, p 6216 (2022)

In 2002, a new class of thymidylate synthase (TS) involved in the de novo synthesis of dTMP named Flavin-Dependent Thymidylate Synthase (FDTS) encoded by the thyX gene was discovered; FDTS is present only in 30% of prokaryote pathogens and not in hum

Externí odkaz: https://doaj.org/article/fa166c10908c426bb800a1ee50e5ea63

Bacterial origin of thymidylate and folate metabolism in Asgard Archaea

Autor: Jonathan Filée, Hubert F. Becker, Lucille Mellottee, Zhihui Li, Jean-Christophe Lambry, Ursula Liebl, Hannu Myllykallio

Little is known about the evolution and biosynthetic function of DNA precursor and the folate metabolism in the Asgard group of archaea. As Asgard occupy a key position in the archaeal and eukaryotic phylogenetic trees, we have exploited very recentl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62e09d6a2ed9411219ff3fc872ee7886
https://doi.org/10.1101/2021.12.15.472764

Photochemical processes in flavo-enzymes as a probe for active site dynamics: TrmFO of Thermus thermophilus

Autor: Pierre Sournia, Bo Zhuang, Rivo Ramodiharilafy, Marten H. Vos, Anastasia Croitoru, Ursula Liebl, Hannu Myllykallio, Alexey Aleksandrov, Lipsa Nag, Jean-Christophe Lambry

Publikováno v: Photochemical & Photobiological Sciences
Photochemical & Photobiological Sciences, 2021, 20 (5), pp.663-670. ⟨10.1007/s43630-021-00052-8⟩
Photochemical & Photobiological Sciences, Royal Society of Chemistry, 2021, 20 (5), pp.663-670. ⟨10.1007/s43630-021-00052-8⟩

International audience; Quenching of flavin fluorescence by electron transfer from neighboring aromatic residues is ubiquitous in flavoproteins. Apart from constituting a functional process in specific light-active systems, time-resolved spectral cha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d61395b4b65c1c206a3392fa37ea488
https://pubmed.ncbi.nlm.nih.gov/33977512

Synthesis of acyclic nucleoside phosphonates targeting Flavin-Dependent Thymidylate Synthase in Mycobacterium tuberculosis

Autor: Hubert F. Becker, Nicolas G. Biteau, Jean-Christophe Lambry, Vincent Roy, Luigi A. Agrofoglio, Hannu Myllykallio

Publikováno v: Bioorganic & Medicinal Chemistry Letters
Bioorganic & Medicinal Chemistry Letters, 2021, 46, pp.116351. ⟨10.1016/j.bmc.2021.116351⟩

International audience; Flavin-Dependent Thymidylate Synthase (FDTS) encoded by ThyX gene was discovered as a new class of thymidylate synthase involved in the de novo synthesis of dTMP named only in 30 % of human pathogenic bacteria. This target was

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c55da1dda58480e595cbb7ee928de022
https://doi.org/10.1016/j.bmc.2021.116351

Activation of the mismatch-specific endonuclease EndoMS/NucS by the replication clamp is required for high fidelity DNA replication

Autor: Yoshizumi Ishino, Hanae Kudo, Asmae Es-Sadik, Caroline L'Hermitte-Stead, Sonoko Ishino, Jean Christophe Lambry, Stéphane Skouloubris, Hannu Myllykallio

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2018, ⟨10.1093/nar/gky460⟩

International audience; The mismatch repair (MMR) system, exemplified by the MutS/MutL proteins, is widespread in Bacteria and Eukarya. However, molecular mechanisms how numerous archaea and bacteria lacking the mutS/mutL genes maintain high replicat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::702e013dd7a9cb60801ba57aac779da2
https://hal.archives-ouvertes.fr/hal-01873297