Light-driven biological actuators to probe the rheology of 3D microtissues

Autor: Adrien Méry, Artur Ruppel, Jean Revilloud, Martial Balland, Giovanni Cappello, Thomas Boudou

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)

The mechanical properties of biological tissues are key to their integrity and function. Here, the authors engineer 3D microtissues from optogenetically modified fibroblasts and use light to quantify tissue elasticity and strain propagation using the

Externí odkaz: https://doaj.org/article/28de9939b8d8471fbcbb768acff51484

Viscoelastic Properties in Cancer: From Cells to Spheroids

Autor: Yara Abidine, Arianna Giannetti, Jean Revilloud, Valérie M. Laurent, Claude Verdier

Publikováno v: Cells, Vol 10, Iss 7, p 1704 (2021)

AFM-based rheology methods enable the investigation of the viscoelastic properties of cancer cells. Such properties are known to be essential for cell functions, especially for malignant cells. Here, the relevance of the force modulation method was i

Externí odkaz: https://doaj.org/article/f1db735c29f84dbdac7d343412243c26

Mechanical properties of 3D tumor spheroids measured by AFM

Autor: A. Giannetti, Jean Revilloud, Claude Verdier

Publikováno v: Computer Methods in Biomechanics and Biomedical Engineering
Congrès de la société de bioméanique (SB2020)
Computer Methods in Biomechanics and Biomedical Engineering, Taylor & Francis, 2020, ⟨10.1080/10255842.2020.1816297⟩

Cancer has remained one of the most indomitable enigmas for scientists over centuries and despite decades of research, it remains a colossal global threat (Chowdhury et al. 2016). Tumor cells have ...

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92e6c4a4ceb983524f397a8b45faa59c
https://doi.org/10.1080/10255842.2020.1816297

C-terminal engineering of CXCL12 and CCL5 chemokines: functional characterization by electrophysiological recordings.

Autor: Antoine Picciocchi, Lina Siaučiūnaiteė-Gaubard, Isabelle Petit-Hartlein, Rabia Sadir, Jean Revilloud, Lydia Caro, Michel Vivaudou, Franck Fieschi, Christophe Moreau, Corinne Vivès

Publikováno v: PLoS ONE, Vol 9, Iss 1, p e87394 (2014)

Chemokines are chemotactic cytokines comprised of 70-100 amino acids. The chemokines CXCL12 and CCL5 are the endogenous ligands of the CXCR4 and CCR5 G protein-coupled receptors that are also HIV co-receptors. Biochemical, structural and functional s

Externí odkaz: https://doaj.org/article/35593aa1e1b64bee9b6ae6067ce66ddf

β2-Adrenergic ion-channel coupled receptors as conformational motion detectors.

Autor: Lydia N Caro, Christophe J Moreau, Jean Revilloud, Michel Vivaudou

Publikováno v: PLoS ONE, Vol 6, Iss 3, p e18226 (2011)

Ion Channel-Coupled Receptors (ICCRs) are artificial proteins comprised of a G protein-coupled receptor and a fused ion channel, engineered to couple channel gating to ligand binding. These novel biological objects have potential use in drug screenin

Externí odkaz: https://doaj.org/article/29bc49e328c94fd691ad1071f53c93b9