Engineered repeat proteins as scaffolds to assemble multi-enzyme systems for efficient cell-free biosynthesis

Autor: Alba Ledesma-Fernandez, Susana Velasco-Lozano, Javier Santiago-Arcos, Fernando López-Gallego, Aitziber L. Cortajarena

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)

Abstract Multi-enzymatic cascades with enzymes arranged in close-proximity through a protein scaffold can trigger a substrate channeling effect, allowing for efficient cofactor reuse with industrial potential. However, precise nanometric organization

Externí odkaz: https://doaj.org/article/f401d3e1623045a58ce4caa078b29717

YY2 in Mouse Preimplantation Embryos and in Embryonic Stem Cells

Autor: Raquel Pérez-Palacios, María Climent, Javier Santiago-Arcos, Sofía Macías-Redondo, Martin Klar, Pedro Muniesa, Jon Schoorlemmer

Publikováno v: Cells, Vol 10, Iss 5, p 1123 (2021)

Yin Yang 2 encodes a mammalian-specific transcription factor (YY2) that shares high homology in the zinc finger region with both YY1 and REX1/ZFP42, encoded by the Yin Yang 1 and Reduced Expression Protein 1/Zinc Finger Protein 42 gene, respectively.

Externí odkaz: https://doaj.org/article/35a41f0687244a7ca35e913da37977a3

Intraparticle Kinetics Unveil Crowding and Enzyme Distribution Effects on the Performance of Cofactor-Dependent Heterogeneous Biocatalysts

Autor: Natalia Comino, Javier Santiago-Arcos, Fernando López-Gallego, Nicolette Czarnievicz, Desiré Di Silvio, Eleftheria Diamanti, Daniel A. Grajales-Hernández, Aitziber L. Cortajarena, Irantzu Llarena

Publikováno v: ACS Catalysis

Multidimensional kinetic analysis of immobilized enzymes is essential to understand the enzyme functionality at the interface with solid materials. However, spatiotemporal kinetic characterization of heterogeneous biocatalysts on a microscopic level

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f215d005d80578885c55a9f1bd85da29
http://europepmc.org/articles/PMC8689653

Immobilization Screening and Characterization of an Alcohol Dehydrogenase and its Application to the Multi-Enzymatic Selective Oxidation of 1,-Omega-Diols

Autor: Eleftheria Diamanti, Aitziber L. Cortajarena, Javier Santiago-Arcos, Susana Velasco-Lozano, Fernando López-Gallego

Alcohol dehydrogenase from Bacillus (Geobacillus) stearothermophilus (BsADH) is a NADH-dependent enzyme catalyzing the oxidation of alcohols, however its thermal and operational stabilities are too low for its long-term use under non-physiological co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::524cd4fcf2f341134643d5ab8f4ea217
https://zenodo.org/record/5508859

Co-immobilization and Colocalization of Multi-Enzyme Systems for the Cell-Free Biosynthesis of Aminoalcohols

Autor: Fernando López-Gallego, Susana Velasco-Lozano, Javier Santiago-Arcos, José A. Mayoral

Publikováno v: Zaguán: Repositorio Digital de la Universidad de Zaragoza
Universidad de Zaragoza
Digital.CSIC. Repositorio Institucional del CSIC
instname
Zaguán. Repositorio Digital de la Universidad de Zaragoza

The manufacturing of aminoalcohols is an appealing transformation for industrial biocatalysis due to their high value as building blocks in synthetic chemistry. By co‐immobilizing dehydrogenases, ω‐transaminases and oxidases on porous carriers,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3460b670932361aec74197c3f2da5e3d
http://zaguan.unizar.es/record/101536