Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Javier Fernandez-Martinez"'
Autor:
Zhanna Hakhverdyan, Kelly R. Molloy, Roman I. Subbotin, Javier Fernandez-Martinez, Brian T. Chait, Michael P. Rout
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100800- (2021)
Summary: We present a comprehensive and robust protocol to track the dynamics of all proteins in a complex in yeast cells. A single member of the protein assembly is tagged and conditionally expressed, minimizing the perturbations to the protein comp
Externí odkaz:
https://doaj.org/article/8f14c84eb3e346c589f3e578b975f3cc
Autor:
Jonathan Markert, Karolin Luger, Hayun Lee, Haidai Hu, Nicholas M.I. Taylor, Javier Fernandez-Martinez, Michael Rout, Nathan Alder
Publikováno v:
Trends in Biochemical Sciences. 48:307-310
Autor:
Toshiya Kozai, Javier Fernandez-Martinez, Trevor van Eeuwen, Paola Gallardo, Larisa E. Kapinos, Adam Mazur, Wenzhu Zhang, Jeremy Tempkin, Radhakrishnan Panatala, Maria Delgado-Izquierdo, Barak Raveh, Andrej Sali, Brian T. Chait, Liesbeth M. Veenhoff, Michael P. Rout, Roderick Y. H. Lim
Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport of specific macromolecules while impeding the exchange of unsolicited material. However, key aspects of this gating mechanism remain controversial. To address this issue, we determined
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c479bc774ff96fe6fb7c8fe212cc3b23
https://doi.org/10.1101/2023.03.31.535055
https://doi.org/10.1101/2023.03.31.535055
Autor:
Digvijay Singh, Neelesh Soni, Ignacia Echeverria, Farhaz Shaikh, Sergey Suslov, Javier Fernandez-Martinez, Paula Upla, Steven Ludtke, Christopher W. Akey, Andrej Sali, Michael P. Rout, Elizabeth Villa
Publikováno v:
Biophysical Journal. 122:55a
Publikováno v:
Methods in Molecular Biology ISBN: 9781071623367
Methods Mol Biol
Methods Mol Biol
Studying protein complexes in vitro requires the production of a relatively pure sample that maintains the full complement, native organization, and function of that complex. This can be particularly challenging to achieve for large, multi-component,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c1e181b188300369539c5572383a09f
https://doi.org/10.1007/978-1-0716-2337-4_1
https://doi.org/10.1007/978-1-0716-2337-4_1
Autor:
Charles M. Rice, Margaret R. MacDonald, Inês M. B. Veiga, Javier Fernandez-Martinez, Bettina Salome Trüeb, Avery Peace, Joseph M. Luna, Eleftherios Michailidis, Tran Thi Nhu Thao, Paul D. Bieniasz, Francisco J. Sánchez-Rivera, Theodora Hatziioannou, Volker Thiel, H.-Heinrich Hoffmann, Fabian Schmidt, Scott W. Lowe, Yingpu Yu, Brandon S. Razooky, Inna Ricardo-Lax, Michael P. Rout, William M. Schneider, Nadine Ebert, Yiska Weisblum, Jérémie Le Pen, Kimberly Schmied, John T. Poirier
Publikováno v:
Science
Ricardo-Lax, Inna; Luna, Joseph M; Tran, Thi Nhu Thao; Le Pen, Jérémie; Yu, Yingpu; Hoffmann, H-Heinrich; Schneider, William M; Razooky, Brandon S; Fernandez-Martinez, Javier; Schmidt, Fabian; Weisblum, Yiska; Trüeb, Bettina Salome; Berenguer Veiga, Inês; Schmied, Kimberly; Ebert, Nadine; Michailidis, Eleftherios; Peace, Avery; Sánchez-Rivera, Francisco J; Lowe, Scott W; Rout, Michael P; ... (2021). Replication and single-cycle delivery of SARS-CoV-2 replicons. Science, 374(6571), pp. 1099-1106. American Association for the Advancement of Science 10.1126/science.abj8430
Ricardo-Lax, Inna; Luna, Joseph M; Tran, Thi Nhu Thao; Le Pen, Jérémie; Yu, Yingpu; Hoffmann, H-Heinrich; Schneider, William M; Razooky, Brandon S; Fernandez-Martinez, Javier; Schmidt, Fabian; Weisblum, Yiska; Trüeb, Bettina Salome; Berenguer Veiga, Inês; Schmied, Kimberly; Ebert, Nadine; Michailidis, Eleftherios; Peace, Avery; Sánchez-Rivera, Francisco J; Lowe, Scott W; Rout, Michael P; ... (2021). Replication and single-cycle delivery of SARS-CoV-2 replicons. Science, 374(6571), pp. 1099-1106. American Association for the Advancement of Science 10.1126/science.abj8430
A tool to study SARS-CoV-2 Work with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires high-level biocontainment facilities, making it important to develop safer molecular tools that can potentially be used under less s
Publikováno v:
Trends Biochem Sci
The nuclear pore complex (NPC) is the massive protein assembly that regulates the transport of macromolecules between the nucleus and the cytoplasm. Recent breakthroughs have provided major insights into the structure of the NPC in different eukaryot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d948d2449d5c01f6476ab49e8380295
https://europepmc.org/articles/PMC8195821/
https://europepmc.org/articles/PMC8195821/
Autor:
Digvijay Singh, Christopher W. Akey, Christna Ouch, Ignacia Echeverria, Ilona Nudelman, Joseph M. Varberg, Zulin Yu, James C. Gumbart, Sue L. Jaspersen, Brian T. Chait, Andrej Sali, Javier Fernandez-Martinez, Steven Ludtke, Michael P. Rout, Elizabeth Villa
Publikováno v:
Biophysical Journal. 121:169a
Autor:
Sarah Keegan, Javier Fernandez-Martinez, Brian T. Chait, John D. Aitchison, David Fenyö, Michael P. Rout, Kelly R. Molloy, Dante M. Lepore, Thurston Herricks, Mary Munson, Zhanna Hakhverdyan, Roman I. Subbotin
Publikováno v:
Mol Cell
Cellular processes are largely carried out by macromolecular assemblies, most of which are dynamic, having components that are in constant flux. One such assembly is the nuclear pore complex (NPC), an ∼50 MDa assembly comprised of ∼30 different p
Autor:
Brian T. Chait, Dante M. Lepore, Mary Munson, Roman I. Subbotin, Kelly R. Molloy, Javier Fernandez-Martinez, Zhanna Hakhverdyan, Michael P. Rout, Sarah Keegan, David Fenyö
Publikováno v:
SSRN Electronic Journal.
Cellular processes are largely carried out by macromolecular assemblies, most of which are dynamic, having components that are in constant flux. One such assembly is the nuclear pore complex (NPC), a ~50 MDa assembly comprised of ~30 different protei