Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Javier Emperador Melero"'
Autor:
Javier Emperador Melero, Aishwarya G. Nadadhur, Desiree Schut, Jan V. Weering, Vivi M. Heine, Ruud F. Toonen, Matthijs Verhage
Publikováno v:
Stem Cell Reports, Vol 8, Iss 3, Pp 659-672 (2017)
Neurons communicate by regulated secretion of chemical signals from synaptic vesicles (SVs) and dense-core vesicles (DCVs). Here, we investigated the maturation of these two secretory pathways in micro-networks of human iPSC-derived neurons. These mi
Externí odkaz:
https://doaj.org/article/3475e3bbeafa4545a487360fa1ee6b53
Autor:
Aishwarya G Nadadhur, Javier Emperador Melero, Marieke Meijer, Desiree Schut, Gerbren Jacobs, Ka Wan Li, J J Johannes Hjorth, Rhiannon M Meredith, Ruud F Toonen, Ronald E Van Kesteren, August B Smit, Matthijs Verhage, Vivi M Heine
Publikováno v:
PLoS ONE, Vol 12, Iss 6, p e0178533 (2017)
Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory-inhibitory activities, which are suitable for single cell analysis. We
Externí odkaz:
https://doaj.org/article/f7a60a49989a499583822389b705e4bf
Publikováno v:
Curr Opin Neurobiol
In a presynaptic nerve terminal, the active zone is composed of sophisticated protein machinery that enables secretion on a sub-millisecond time scale and precisely targets it towards postsynaptic receptors. The past two decades have provided deep in
Publikováno v:
Neuroscience, 420, 32-40. Elsevier Limited
Emperador-Melero, J, Toonen, R F & Verhage, M 2019, ' Vti Proteins : Beyond Endolysosomal Trafficking ', Neuroscience, vol. 420, pp. 32-40 . https://doi.org/10.1016/j.neuroscience.2018.11.014
Emperador-Melero, J, Toonen, R F & Verhage, M 2019, ' Vti Proteins : Beyond Endolysosomal Trafficking ', Neuroscience, vol. 420, pp. 32-40 . https://doi.org/10.1016/j.neuroscience.2018.11.014
Vti proteins are conserved from yeast to humans and regulate intracellular membrane trafficking by providing one specific SNARE domain, the Qb SNARE, to the four helical SNARE bundle that drives membrane fusion. Two mammalian Vti genes, Vti1a and Vti
Autor:
Javier Emperador-Melero, Giovanni de Nola, Tom Kirchhausen, Man Yan Wong, Shan-Shan Wang, Pascal S. Kaeser, Hajnalka Nyitrai
Publikováno v:
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
The active zone of a presynaptic nerve terminal defines sites for neurotransmitter release. Its protein machinery may be organized through liquid–liquid phase separation, a mechanism for the formation of membrane-less subcellular compartments. Here
Publikováno v:
eLife
eLife, Vol 10 (2021)
eLife, Vol 10 (2021)
It has long been proposed that leukocyte common antigen-related receptor protein tyrosine phosphatases (LAR-RPTPs) are cell-adhesion proteins that control synapse assembly. Their synaptic nanoscale localization, however, is not established, and synap
Autor:
Shan-Shan Wang, Tom Kirchhausen, Pascal S. Kaeser, Giovanni de Nola, Man Yan Wong, Javier Emperador-Melero
Liquid-liquid phase separation enables the assembly of membrane-less subcellular compartments, but testing its biological functions has been difficult. The presynaptic active zone, protein machinery in nerve terminals that defines sites for neurotran
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::01036c5389007dadd68b59c4ce3da5e5
https://doi.org/10.1101/2020.10.28.357574
https://doi.org/10.1101/2020.10.28.357574
Autor:
Javier Emperador-Melero, Ruud F. Toonen, Gabriele Fischer von Mollard, Matthijs Verhage, Jan R.T. van Weering, Christian Bollmann, Vincent Huson
Publikováno v:
Emperador-Melero, J, Huson, V, van Weering, J, Bollmann, C, Fischer von Mollard, G, Toonen, R F & Verhage, M 2018, ' Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi ', Nature Communications, vol. 9, no. 1, 3421 . https://doi.org/10.1038/s41467-018-05699-z
Nature Communications, Vol 9, Iss 1, Pp 1-17 (2018)
Nature Communications
Nature Communications, 9(1):3421. Nature Publishing Group
Emperador-Melero, J, Huson, V, van Weering, J, Bollmann, C, Fischer von Mollard, G, Toonen, R F & Verhage, M 2018, ' Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi ', Nature Communications, vol. 9, 3421, pp. 1-17 . https://doi.org/10.1038/s41467-018-05699-z
Nature Communications, 9:3421, 1-17. Nature Publishing Group
Nature Communications, Vol 9, Iss 1, Pp 1-17 (2018)
Nature Communications
Nature Communications, 9(1):3421. Nature Publishing Group
Emperador-Melero, J, Huson, V, van Weering, J, Bollmann, C, Fischer von Mollard, G, Toonen, R F & Verhage, M 2018, ' Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi ', Nature Communications, vol. 9, 3421, pp. 1-17 . https://doi.org/10.1038/s41467-018-05699-z
Nature Communications, 9:3421, 1-17. Nature Publishing Group
The SNAREs Vti1a/1b are implicated in regulated secretion, but their role relative to canonical exocytic SNAREs remains elusive. Here, we show that synaptic vesicle and dense-core vesicle (DCV) secretion is indeed severely impaired in Vti1a/b-deficie
Autor:
Jan R.T. van Weering, Javier Emperador Melero, Matthijs Verhage, Desiree Schut, Ruud F. Toonen, Aishwarya G. Nadadhur, Vivi M. Heine
Publikováno v:
Stem Cell Reports, Vol 8, Iss 3, Pp 659-672 (2017)
Emperador Melero, J, Nadadhur, A G, Schut, D, Weering, J V, Heine, V M, Toonen, R F & Verhage, M 2017, ' Differential Maturation of the Two Regulated Secretory Pathways in Human iPSC-Derived Neurons ', Stem cell reports, vol. 8, no. 3, pp. 659-672 . https://doi.org/10.1016/j.stemcr.2017.01.019
Emperador Melero, J, Nadadhur, A G, Schut, D, Weering, J V, Heine, V M, Toonen, R F & Verhage, M 2017, ' Differential Maturation of the Two Regulated Secretory Pathways in Human iPSC-Derived Neurons ', Stem Cell Reports, vol. 8, no. 3, pp. 659-672 . https://doi.org/10.1016/j.stemcr.2017.01.019
Stem Cell Reports
Stem Cell Reports, 8(3), 659-672. Cell Press
Stem cell reports, 8(3), 659-672. Cell Press
Emperador Melero, J, Nadadhur, A G, Schut, D, Weering, J V, Heine, V M, Toonen, R F & Verhage, M 2017, ' Differential Maturation of the Two Regulated Secretory Pathways in Human iPSC-Derived Neurons ', Stem cell reports, vol. 8, no. 3, pp. 659-672 . https://doi.org/10.1016/j.stemcr.2017.01.019
Emperador Melero, J, Nadadhur, A G, Schut, D, Weering, J V, Heine, V M, Toonen, R F & Verhage, M 2017, ' Differential Maturation of the Two Regulated Secretory Pathways in Human iPSC-Derived Neurons ', Stem Cell Reports, vol. 8, no. 3, pp. 659-672 . https://doi.org/10.1016/j.stemcr.2017.01.019
Stem Cell Reports
Stem Cell Reports, 8(3), 659-672. Cell Press
Stem cell reports, 8(3), 659-672. Cell Press
Summary Neurons communicate by regulated secretion of chemical signals from synaptic vesicles (SVs) and dense-core vesicles (DCVs). Here, we investigated the maturation of these two secretory pathways in micro-networks of human iPSC-derived neurons.
Publikováno v:
Molecular Cell. 73:859-860
Wu et al. (2019) establish that the active zone proteins RIM and RIM-BP undergo liquid-liquid phase separation to tether Ca2+ channels. This important finding sets a new framework to study assembly and function of the presynaptic nerve terminal.