The transport activity of the multidrug ABC transporter BmrA does not require a wide separation of the nucleotide-binding domains.

Autor: Di Cesare M; Bacterial Nucleotide-Binding Proteins Team, Molecular Microbiology and Structural Biochemistry (MMSB), UMR 5086 CNRS/University of Lyon, Lyon, France., Kaplan E; Bacterial Nucleotide-Binding Proteins Team, Molecular Microbiology and Structural Biochemistry (MMSB), UMR 5086 CNRS/University of Lyon, Lyon, France., Rendon J; CNRS, Aix-Marseille Université, BIP, IMM, Marseille, France., Gerbaud G; CNRS, Aix-Marseille Université, BIP, IMM, Marseille, France., Valimehr S; Ian Holmes Imaging Center and Department of Biochemistry and Pharmacology and ARC Centre for Cryo-Electron Microscopy of Membrane Proteins, Bio21 Institute, University of Melbourne, Parkville, VIC, Australia., Gobet A; Drug Resistance and Membrane Proteins Team, Molecular Microbiology and Structural Biochemistry (MMSB), UMR 5086 CNRS/University of Lyon, Lyon, France., Ngo TT; Bacterial Nucleotide-Binding Proteins Team, Molecular Microbiology and Structural Biochemistry (MMSB), UMR 5086 CNRS/University of Lyon, Lyon, France., Chaptal V; Drug Resistance and Membrane Proteins Team, Molecular Microbiology and Structural Biochemistry (MMSB), UMR 5086 CNRS/University of Lyon, Lyon, France., Falson P; Drug Resistance and Membrane Proteins Team, Molecular Microbiology and Structural Biochemistry (MMSB), UMR 5086 CNRS/University of Lyon, Lyon, France., Martinho M; CNRS, Aix-Marseille Université, BIP, IMM, Marseille, France., Dorlet P; CNRS, Aix-Marseille Université, BIP, IMM, Marseille, France., Hanssen E; Ian Holmes Imaging Center and Department of Biochemistry and Pharmacology and ARC Centre for Cryo-Electron Microscopy of Membrane Proteins, Bio21 Institute, University of Melbourne, Parkville, VIC, Australia., Jault JM; Bacterial Nucleotide-Binding Proteins Team, Molecular Microbiology and Structural Biochemistry (MMSB), UMR 5086 CNRS/University of Lyon, Lyon, France. Electronic address: jean-michel.jault@ibcp.fr., Orelle C; Bacterial Nucleotide-Binding Proteins Team, Molecular Microbiology and Structural Biochemistry (MMSB), UMR 5086 CNRS/University of Lyon, Lyon, France. Electronic address: cedric.orelle@ibcp.fr.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2024 Jan; Vol. 300 (1), pp. 105546. Date of Electronic Publication: 2023 Dec 09.

Waste or die: The price to pay to stay alive.

Autor: Orelle C; University of Lyon, CNRS, UMR5086 'Molecular Microbiology and Structural Biochemistry', IBCP, 7 Passage du Vercors, F-69367, Lyon, France. Electronic address: cedric.orelle@ibcp.fr., Schmitt L; Institute of Biochemistry, Heinrich Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany. Electronic address: lutz.schmitt@hhu.de., Jault JM; University of Lyon, CNRS, UMR5086 'Molecular Microbiology and Structural Biochemistry', IBCP, 7 Passage du Vercors, F-69367, Lyon, France. Electronic address: jean-michel.jault@ibcp.fr.

Publikováno v: Trends in microbiology [Trends Microbiol] 2023 Mar; Vol. 31 (3), pp. 233-241. Date of Electronic Publication: 2022 Sep 30.

Structural Insights into the Catalytic Cycle of a Bacterial Multidrug ABC Efflux Pump.

Autor: Javed W; Molecular Microbiology & Structural Biochemistry (MMSB) UMR 5086, CNRS/University of Lyon, Lyon 69367, France; IBS, Univ. Grenoble Alpes, CNRS, CEA, Grenoble 38000, France; Institut Max Von Laue Paul Langevin (ILL), Grenoble 38000, France., Vallet S; Molecular Microbiology & Structural Biochemistry (MMSB) UMR 5086, CNRS/University of Lyon, Lyon 69367, France., Clement MP; Molecular Microbiology & Structural Biochemistry (MMSB) UMR 5086, CNRS/University of Lyon, Lyon 69367, France., Le Roy A; IBS, Univ. Grenoble Alpes, CNRS, CEA, Grenoble 38000, France., Moulin M; Institut Max Von Laue Paul Langevin (ILL), Grenoble 38000, France., Härtlein M; Institut Max Von Laue Paul Langevin (ILL), Grenoble 38000, France., Breyton C; IBS, Univ. Grenoble Alpes, CNRS, CEA, Grenoble 38000, France., Burlet-Schiltz O; Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, Toulouse, 31000, France., Marcoux J; Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, Toulouse, 31000, France. Electronic address: julien.marcoux@ipbs.fr., Orelle C; Molecular Microbiology & Structural Biochemistry (MMSB) UMR 5086, CNRS/University of Lyon, Lyon 69367, France. Electronic address: cedric.orelle@ibcp.fr., Ebel C; IBS, Univ. Grenoble Alpes, CNRS, CEA, Grenoble 38000, France. Electronic address: christine.ebel@ibs.fr., Martel A; Institut Max Von Laue Paul Langevin (ILL), Grenoble 38000, France. Electronic address: martela@ill.fr., Jault JM; Molecular Microbiology & Structural Biochemistry (MMSB) UMR 5086, CNRS/University of Lyon, Lyon 69367, France. Electronic address: jean-michel.jault@ibcp.fr.

Publikováno v: Journal of molecular biology [J Mol Biol] 2022 May 15; Vol. 434 (9), pp. 167541. Date of Electronic Publication: 2022 Mar 12.

Substrate-bound and substrate-free outward-facing structures of a multidrug ABC exporter.

Autor: Chaptal V; Drug Resistance and Membrane Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Zampieri V; Drug Resistance and Membrane Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Wiseman B; Drug Resistance and Membrane Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France.; Department of Biochemistry and Biophysics, Arrhenius Laboratories for Natural Sciences, Stockholm University, Stockholm, Sweden., Orelle C; Bacterial Nucleotide-Binding Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Martin J; Modeling Biological Macromolecules Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Nguyen KA; University of Grenoble Alpes, INSERM, LRB, 38000 Grenoble, France., Gobet A; Drug Resistance and Membrane Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Di Cesare M; Bacterial Nucleotide-Binding Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Magnard S; Drug Resistance and Membrane Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Javed W; Bacterial Nucleotide-Binding Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Eid J; Drug Resistance and Membrane Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Kilburg A; Drug Resistance and Membrane Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Peuchmaur M; University of Grenoble Alpes, CNRS, DPM UMR 5063, 38041 Grenoble, France., Marcoux J; Institut de Pharmacologie et de Biologie Structurale (IPBS), UMR 5089, Université de Toulouse, CNRS, UPS, 31000 Toulouse, France., Monticelli L; Modeling Biological Macromolecules Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Hogbom M; Department of Biochemistry and Biophysics, Arrhenius Laboratories for Natural Sciences, Stockholm University, Stockholm, Sweden., Schoehn G; University of Grenoble Alpes, CEA, CNRS, IBS, F-38000 Grenoble, France., Jault JM; Bacterial Nucleotide-Binding Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France., Boumendjel A; University of Grenoble Alpes, INSERM, LRB, 38000 Grenoble, France., Falson P; Drug Resistance and Membrane Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS UMR 5086, University of Lyon, IBCP, 7, passage du Vercors, 69367 Lyon, France.

Publikováno v: Science advances [Sci Adv] 2022 Jan 28; Vol. 8 (4), pp. eabg9215. Date of Electronic Publication: 2022 Jan 26.

Identification of novel inhibitors of the ABC transporter BmrA.

Autor: Serçinoğlu O; Department of Bioengineering, Recep Tayyip Erdogan University, Fener 53100, Rize, Turkey., Senturk D; Department of Bioengineering, Marmara University, Kadikoy 34722, Istanbul, Turkey., Altinisik Kaya FE; Department of Bioengineering, Marmara University, Kadikoy 34722, Istanbul, Turkey., Avci FG; Department of Bioengineering, Uskudar University, Uskudar 34662, Istanbul, Turkey., Frlan R; University of Ljubljana, Faculty of Pharmacy, Aškerčeva 7, 1000 Ljubljana, Slovenia., Tomašič T; University of Ljubljana, Faculty of Pharmacy, Aškerčeva 7, 1000 Ljubljana, Slovenia., Ozbek P; Department of Bioengineering, Marmara University, Kadikoy 34722, Istanbul, Turkey., Orelle C; University of Lyon, CNRS, UMR5086 'Molecular Microbiology and Structural Biochemistry', 7 passage du Vercors, 69367 Lyon Cedex 7, France., Jault JM; University of Lyon, CNRS, UMR5086 'Molecular Microbiology and Structural Biochemistry', 7 passage du Vercors, 69367 Lyon Cedex 7, France., Sariyar Akbulut B; Department of Bioengineering, Marmara University, Kadikoy 34722, Istanbul, Turkey. Electronic address: berna.akbulut@marmara.edu.tr.

Publikováno v: Bioorganic chemistry [Bioorg Chem] 2020 Dec; Vol. 105, pp. 104452. Date of Electronic Publication: 2020 Nov 04.