Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Jason E. Kreutz"'
Autor:
Yuling Qin, Jason E. Kreutz, Thomas Schneider, Gloria S. Yen, Eleanor S. Shah, Li Wu, Daniel T. Chiu
Publikováno v:
Lab on a Chip. 22:4729-4734
Hot embossing is a cost-effective and flexible fabrication technology with high replication accuracy for feature sizes as small as 50 nm. Here we develop a reinforced polydimethylsiloxane (PDMS) mold for hot embossing of cyclic olefin polymer (COP) s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a7b6dfecb8b720bc606696e639f7ab2
https://doi.org/10.1039/d2lc00857b
https://doi.org/10.1039/d2lc00857b
Autor:
Alison M Thompson, Jordan L Smith, Luke D Monroe, Jason E Kreutz, Thomas Schneider, Bryant S Fujimoto, Daniel T Chiu, Jerald P Radich, Amy L Paguirigan
Publikováno v:
PLoS ONE, Vol 13, Iss 5, p e0196801 (2018)
Cancer is a heterogeneous disease, and patient-level genetic assessments can guide therapy choice and impact prognosis. However, little is known about the impact of genetic variability within a tumor, intratumoral heterogeneity (ITH), on disease prog
Externí odkaz:
https://doaj.org/article/ff31e625eb914d559a7bbb87f0ea1654
Publikováno v:
Methods in Molecular Biology ISBN: 9781071618028
Digital nucleic acid quantitation methods show excellent sensitivity and specificity for pathogen detection. Droplet digital PCR (ddPCR) is the most advanced digital nucleic acid quantitation method and has been commercialized, but is not suitable fo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::58bd3c9b9fc28ebade8799391cd2aa6f
https://doi.org/10.1007/978-1-0716-1803-5_15
https://doi.org/10.1007/978-1-0716-1803-5_15
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2393
Digital nucleic acid quantitation methods show excellent sensitivity and specificity for pathogen detection. Droplet digital PCR (ddPCR) is the most advanced digital nucleic acid quantitation method and has been commercialized, but is not suitable fo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b6747138c4f32b878a19fa8ffe0dda03
https://pubmed.ncbi.nlm.nih.gov/34837185
https://pubmed.ncbi.nlm.nih.gov/34837185
Autor:
Jason E. Kreutz, Alison M. Thompson, Jeannette P. Staheli, Qinghua Feng, Michael R. Dyen, Allison M. Sheen, Jiasi Wang, Daniel T. Chiu
Publikováno v:
Lab on a Chip. 19:1035-1040
Digital nucleic acid amplification and detection methods provide excellent sensitivity and specificity and allow absolute quantification of target nucleic acids. Isothermal methods such as digital loop-mediated isothermal amplification (digital LAMP)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df62055486dfc1ffcf813bbf59a20518
https://doi.org/10.1039/c8lc01223g
https://doi.org/10.1039/c8lc01223g
Autor:
Daniel T. Chiu, Halia Haynes, Jeannette P. Staheli, Jason E. Kreutz, Kara Shibley, Yuling Qin, Thomas Schneider, Bryant S. Fujimoto, Qiongzheng Hu, Gloria S. Yen, Jingang Wang, Andrew Wu, Bob Weng, Qinghua Feng, Jiasi Wang, Rachel L. Winer
Publikováno v:
Anal Chem
Cervical cancer is the fourth-leading cause of cancer deaths among women worldwide and most cases occur in developing countries. Detection of high-risk (HR) HPV, the etiologic agent of cervical cancer, is a primary screening method for cervical cance
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4b6abb4a97b049d5f71355f9cfa15e4
https://europepmc.org/articles/PMC8020467/
https://europepmc.org/articles/PMC8020467/
Autor:
Robert A. Smith, Li Wu, Jason E. Kreutz, Geoffrey S. Gottlieb, Jingang Wang, Ming Chang, Dana N. Raugi, Jiasi Wang, Yuling Qin, Alison M. Thompson, Daniel T. Chiu, Allison M. Sheen, Shihan Xu
Publikováno v:
Lab on a Chip. 18:3501-3506
Quantitative detection of RNA is important in molecular biology and clinical diagnostics. Nucleic acid sequence-based amplification (NASBA), a single-step method to amplify single-stranded RNA, is attractive for use in point-of-care (POC) diagnostics
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73d40009ac3ef18b1522d5647936cc90
https://doi.org/10.1039/c8lc00956b
https://doi.org/10.1039/c8lc00956b
We present a method to determine the concentration of nucleic acids in a sample by partitioning it into droplets with a nonuniform volume distribution. This digital PCR method requires no special equipment for partitioning, unlike other methods that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2b4146ecc79a7a70f5fbb9ae1d84ae7
https://europepmc.org/articles/PMC6383768/
https://europepmc.org/articles/PMC6383768/
Publikováno v:
Analytical Chemistry. 85:3476-3482
Droplet microfluidics, which involves micrometer-sized emulsion droplets on a microfabricated platform, is an active research endeavor that evolved out of the larger field of microfluidics. Recently, this subfield of microfluidics has started to attr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fa14d54e489851d04d335d479420fb2
https://doi.org/10.1021/ac400257c
https://doi.org/10.1021/ac400257c
Autor:
Stephanie E. McCalla, Mikhail A. Karymov, Rustem F. Ismagilov, Bing Sun, Jason E. Kreutz, Feng Shen
Publikováno v:
Analytical Chemistry. 85:1540-1546
Here we used a SlipChip microfluidic device to evaluate the performance of digital reverse transcription-loop-mediated isothermal amplification (dRT-LAMP) for quantification of HIV viral RNA. Tests are needed for monitoring HIV viral load to control
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e2ce6f6593e45f96dd540695c69a6ef
https://doi.org/10.1021/ac3037206
https://doi.org/10.1021/ac3037206